Pseudoaerumnosa obovata, Vilkamaa & Halenius & Ševčík, 2019

Vilkamaa, Pekka, Halenius, Pentti & Ševčík, Jan, 2019, Review of Pseudoaerumnosa Rudzinski (Diptera, Sciaridae), with the description of twenty-four new species, Zootaxa 4656 (1), pp. 1-42 : 29-34

Pseudoaerumnosa obovata sp. n.

Figs 1 A , 15 B , 16 , 17 , 21 A

Material studied. Holotype male. BRUNEI, Tutong, Ladan Hills Forest Reserve , 4.58°N, 114.50°E, logged forest, Malaise trap, XII.1992 – II.1993, M.J. Sahat (in MZH) . Paratypes. BRUNEI, same data as holotype, 7 males (5 MZH, 2 in SDEI) ; same data as previous but primary forest, 1 male (in MZH) ; Bandar Seri Bedawan UBD Campus , secondary forest, Malaise trap, 19–22.I.2014, Ševčík & Kaspřák, 5 males (2 in MZH, 3 in UBDC) ; MA- LAYSIA, Sarawak, Gunung Mulu N.P., alluvial forest near base camp, Malaise trap, 13.II–1.III.1979, N.M. Collins, 1 male (B.M. 1977-543, in BMNH) ; Selangor, Ulu Gombak Field Studies Centre , 3.31°N, 101.75°E, forest, Malaise trap, 1. VI.2012, J. Wilson, 1 male (Bold system GMMGK092-14 , in CBG), same data but 10.VIII.2012, 1 male ( GMMGP265-14 , in CBG) ; same data but 21.IX.2012, 1 male ( GMMGJ412-14 , in CBG) ; same data but 28.IX.2012, 2 males ( GMMGT1047-14 and GMMGT1131-14 , in CBG) ; THAILAND, Khao Yai N.P., 780 m, moist semi-evergreen forest, Malaise trap, 16–20.IV.1990, B.V. Brown, 4 males (in AMNH) ; same data as previous but 20–24.IV.1990, 1 male ( MZH) ; Kanchanaburi, Khuean Srinagarinda N.P., Chong Kraborg , 14°29.972’N, 98°53.036’E, 210 m, Malaise trap, Boonkam & Phumarin, 6 males (in QSBG) ; same data but 25.IX–2.X.2008, 3 males (in MZH) ; THAILAND, Ban Mae Lao , slope near brook, Malaise trap 2, 19–26.v.2017, M. Mantič & T. Sikora, 2 males (in MZH and JSL-OUC) ; Ban Mae Lao , Malaise trap 4, 19–26.v.2017, M. Mantič & T. Sikora, 2 males (in MZH and JSL-OUC) ; Surat Thani, Khao sok, N.P., Bang Huaraed , 8°54.555’N, 98°30.622’E, 122 m, Malaise trap, 14–21.IV.2009, Pongphan, 6 males (T4571, in QSBG) ; same data but 16–23.XII.2008, 1 male (T3889, in MZH) ; Phetchabun, Thung Salaeng Luang N.P., Kaeng Wang Nam Yen , 16°37.531’N, 100°53.745’E, Malaise trap, 29.XI–6.XII.2006, Tiger Project, 1 male (T1165, in MZH) ; Nakhon Si Thammarat, Namtok Yong N.P., 8°10.434’N, 99°44.508’E, 80 m, behind campground lavatory, Malaise trap, 22–29.X.2008, K. U-prai, 1 male (T4235, in MZH) ; PAPUA NEW GUINEA, Madang, Wanang CTFS plot, 5.2254°S, 145.081°E, forest, Malaise trap, 2.VIII.2014, P. Toko, 1 male ( GMNGD292-16 , in CBG) ; same data but 27.IX.2014, 4 males ( GMNGH909-16 , GMNGH922-16 , GMNGH924-16 and GMNGH982-16 , in CBG) .

DNA sequences. The DNA sequences of COI barcode region taken from four paratypes are deposited in Gen- Bank. The Accession Numbers are provided in Table 1 . This species (a paratype) was included in the phylogenetic analysis by Vilkamaa et al. (2018) as Pseudoaerumnosa sp. 1.

Description. Male. Head. Brown, antenna concolorous with face, maxillary palpus pale brown. Eye bridge 2–4 facets wide. Face with 4–11 dark long setae. Clypeus non-setose. Mouthparts strongly reduced. Maxillary palpus with one segment, with 2–6 pointed setae, with a large dorsal patch of sensilla.Antenna short, antennal flagellomeres laterally flattened, asymmetrical in relation to their necks, frontal part larger; densely setose, setae curved, body of 4th flagellomere 1.35–1.95x as long as wide, the longest setae much shorter than the width of the flagellomere; neck shorter than wide. Thorax. Unicolorous dark brown; setae dark. Anterior pronotum with 1–6 setae. Proepisternum with 1–7 setae. Scutellum with 4 moderately long and many short setae. Wing. Fumose. Length 1.8–2.2 mm. Width/ length 0.50–0.55. Anal lobe rather large. Hind margin with dorsal and ventral setae. Veins distinct. R1/R 1.25–1.65. c/w 0.60–0.90. bM longer or shorter than r-m, stM longer or shorter than M-fork. stCu longer, as long as or shorter than bM. R5 joining c at about base of M-fork. R1 with dorsal and ventral setae, bM, r-m and StCu non-setose. Haltere pale brown. Legs, Fig. 1 A . Yellow. Fore tibial organ unmodified, with some fine setae. Fore tibial spur as long as the tibial width. Abdomen. Pale brown; setae moderately long, dark, and robust. Hypopygium, Figs 15 B , 16 , 17 , 21 A . Brown, like the abdomen; setae dark. Gonocoxa as long as gonostylus. Gonocoxae fused, intergonocoxal area broad and short, microtrichose and non-setose medially, ventromedial margin roundish and distinct. Setosity of gonocoxa long and dense, slightly shorter at medial margin. Apicomedial seta poorly differentiated. Gonostylus elongated, impressed dorsally and ventrally, with a large medial lobe, with a large apical tooth with a small megaseta attached to its ventral side, with 2 megasetae subapically near the apical tooth, and 3 on the medial lobe, megasetae slightly curved, slender and hyalinous. Tegmen ovale apicolaterally, hyalinous, weakly sclerotized laterally, with tiny aedeagal teeth. Aedeagal apodeme broad and rather long, with distinct apical fork.

Female. Unknown.

Discussion. Pseudoaerumnosa obovata sp. n. has a wide distribution and shows slight variation between specimens from different localities ( Figs 15–17 ). The barcoded specimens from Selangor, Malaysia and those from Papua New Guinea are identical in their morphology and COI sequence with the Brunei specimens, whereas there is only 95 % congruence in the barcode COI sequence between the material from Khao Yai, Thailand and that from from Brunei. It may be that P. obovata in the present concept is a species complex which needs a more detailed study using molecular and advanced microscopy methods.

Pseudoaerumnosa obovata shares with many species a similar gonostylar structure with 2 sub-basal megasetae and a medial lobe with 3 to 6 megasetae. Pseudoaerumnosa obovata is very similar to P. collicola sp. n. and P. junciseta sp. n. in having an apically and laterally roundish tegmen and both dorsally and ventrally setose hind margin of wing. Pseudoarumnosa collicola differs from the two other species in having a broader gonostylus with a rather short apical tooth which is slightly constricted sub-basally and in having a strongly roundish tegmen, whereas P. junciseta is distinguished in having a very narrow apical tooth and megasetae of the gonostylus, in having an evenly narrowed apical tooth and in having its wing vein R1 joining c beyond the level of the base of the M-fork.

Etymology. The name is derived from the Latin words ob-, towards, and ovum, egg, referring to the subovale tegmen of the hypopygium.

Figures

FIGURE 1. A. Apical part of fore tibia, prolateral. B. 4 th antennal flagellomere, frontal. C. 4th antennal flagellomere, lateral. A: Pseudoaerumnosa obovata sp. n. (paratype from Ladan). B, C: P. clavidactyla sp. n. (paratypes from Danum Valley). Scale 0.10 mm.

FIGURE 15. Hypopygium, ventral. A: Pseudoaerumnosa junciseta sp. n. (holotype). B: P. obovata sp. n. (holotype). Scale 0.10 mm.

FIGURE 16. Hypopygium, ventral. A: Pseudoaerumnosa obovata sp. n. (paratype from Thailand, Ban Mae Lao). B: P. obovata sp. n. (paratype from Thailand, Khao Yai). Scale 0.10 mm.

FIGURE 17. Hypopygium, ventral. A: Pseudoaerumnosa obovata sp. n. (paratype from Thailand, Khao Sok). B: P. obovata sp. n. (paratype from Thailand, Kanchanaburi). Scale 0.10 mm.

FIGURE 21. Gonostylus, ventral. A: Pseudoaerumnosa obovata sp. n. (holotype). B: P. pilicaudata sp. n. (holotype). C: P. quadriquetra sp. n. (holotype). D: P. tenuidens sp. n. (holotype). Scale 0.10 mm.