Kerivoula minuta Miller, 1898

Voon-Ching Lim, Rosli Ramli, Subha Bhassu & John-James Wilson, 2017, A checklist of the bats of Peninsular Malaysia and progress towards a DNA barcode reference library, PLoS ONE 12 (7), pp. 1-65 : 38-39

Kerivoula minuta Miller, 1898

Kerivoula minuta Miller, 1898: 321 ; Lay Song Hong , Trang, south THAILAND (Dr. W. L. Abbott, collector; USNM 83547 ) [ 174].

Common English name: Least Woolly Bat

Barcode Index Number: BOLD: AAC 1298 (9 DNA barcodes from Peninsular Malaysia; Fig 6 )

Remarks: DNA barcodes recorded as K. minuta are associated with six BINs, BOLD: AAC1296, BOLD:AAC1297, BOLD:AAC1298, BOLD:AAC1299, BOLD:ACF4510, and BOLD:ACF451 ( Fig 6 ). Khan [ 175] reported 4.44% of divergence in cytochrome b mtDNA between K. minuta from Peninsular Malaysia and Malaysian Borneo (Sabah and Sarawak) with no shared haplotypes. Our NJ analysis also showed a divergence between K. minuta from Peninsular Malaysia and Borneo ( Fig 6 ). The taxon occurring in Peninsular Malaysia represents K. minuta based on the type locality.

IUCN status: Near Threatened

Recorded at: Johor: Endau Rompin National Park (BM422-04 and ABRSS347-06 [ 4]), Gunung Panti and Labis Forest Reserve [ 100]; Selangor: Ulu Gombak and Hulu Langat [ 32]; Pahang: Krau Wildlife Reserve and Lakum [ 32], Kuala Atok, National Park [ 32, 44], Bukit Ibam, Kenong and Gunung Aais [ 100]; Perak: Maxwell Hill [ 32], Temengor Forest Reserve [ 46, 47], Royal Belum State Park [ 66]; Kedah: Bukit Hijau and Ulu Muda Forest Reserve [ 32, 57, 100]; Kelantan: Gua Musang [ 61, 62]; Negeri Sembilan: Gunung Angsi Forest Reserve [ 100].

K. minuta has been recorded in the understory of lowland forests and disturbed areas [ 14].

Kerivoulapapillosa and K. lenis . K. lenis iscloselyassociatedwith K. papillosa [ 9] butthe former has a smaller skull and smaller teeth, shorter muzzle and narrower palate [ 169, 176]. The two species are 10.85% divergent in cytochrome b mtDNA and possess unique karyotypic characters: K. papillosa has a diploid number of chromosomes = 38 and fundamental number = 54 whereas K. lenis has a diploid number of chromosomes = 38 and fundamental number = 52 [ 32, 175]. Analyses of COI mtDNA by Francis et al. [ 4] suggested that there are at least four distinct clusters among specimens recorded as K. papillosa and K. lenis . Douangboubpha et al. [ 170] reported that specimens from Thailand referred as K. papillosa represent five morphological forms but only three distinct clusters based on COI mtDNA analyses.

Figures

Fig 6. Neighbour-joining tree showing all available DNA barcodes for species in family Vespertilionidae reported from Peninsular Malaysia. The percentage of pseudoreplicate trees (±70%) in which the DNA barcodes clustered together in the bootstrap test (500 pseudoreplicates) are shown above the branches. Abbreviation as follows: PM = Peninsular Malaysia,VN = Vietnam, BN = Borneo (including Sabah & Sarawak of East Malaysia, Brunei and Kalimantan Indonesia), TH = Thailand, LA = Laos, SM = Sumatera Indonesia, JV = Java Indonesia, CH = China, CM = Cambodia. https://doi.org/10.1371/journal.pone.0179555.g006