Periglischrus torrealbai Machado-Allison, 1965

Gomes-AlmeidaK, Brenda Karolina, DiórioK, Gabriel Félix, CostaK, Samuel Geremias dos Santos & PepatoK, Almir Rogério, 2024, DNA barcoding, visual-guide resource, new localities and host associations of genus Periglischrus Oudemans, 1902 (Acari: Mesostigmata, Spinturnicidae) from Minas Gerais, Brazil, Acarologia 1902 (2), pp. 425-462 : 453-457

Periglischrus torrealbai Machado-Allison ( Figures 17–19 )

Periglischrus torrealbai Machado-Allison, 1965a:276–279 .

Periglischrus inflatiseta Furman, 1966: 134–135 .

Specimens examined — 2♂ (UFMG AC 220041, 221030) and 1 protonymph (UFMG

AC 220054) on Phyllostomus discolor (3 ex.): Brazil, Minas Gerais, Lagoa Santa, Lapinha cave, PE Sumidouro, - 19.5616° S, - 43.959° E, 11 Aug. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence[voucher code]: OP964439[UFMG AC 221030], OP964442[UFMG

AC 220041], OP964444[UFMG AC 220054]); 1♂ (UFMG AC 221129) on Tonatia bidens (1 ex.): Brazil, Minas Gerais, Pains, Mastodonte cave, - 20.4270° S, - 45.6322° E, 27 Jan. 2021, collected by A. Tahara et al. (COX1 sequence[voucher code]: OP964443[UFMG AC 221129]);

4♂ (UFMG AC 220137, 220138, 220823, 220139) on Phyllostomus hastatus (PESNT112): Brazil, Minas Gerais, Rio Pardo de Minas, Mosquito cave (unregistered), PE Serra Nova e Talhado, - 15.6545° S, - 42.7335° E, 16 Dec. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence[voucher code]: OP964440[UFMG AC 220139]); ♀ (UFMG AC 220043)

on Phyllostomus discolor (1 ex.): Brazil, Minas Gerais, Lagoa Santa, Lapinha cave, PE Sumidouro, - 19.5616° S, - 43.959° E, 11 Aug. 2021, collected by B. Gomes-Almeida et al. (COX1 sequence [voucher code]: OP964441[UFMG AC 220043]).

Barcode sequences — OP964441–44 ( Table 1).

Distribution — Brazil, Colombia, Costa Rica, Panama, Peru and Venezuela ( Gettinger 2018 ; Beron 2020).

Hosts and records from Brazil — Artibeus planirostri s (Spix, 1823): Ceará ( Almeida et al. 2016b); Lophostoma silviculu m: Mato Grosso do Sul ( Silva and Graciolli 2013 ; Silva et al. 2017); Phyllostomus discolor (Wagner, 1843) : Ceará ( Almeida et al. 2016b ; Almeida et al. 2018); Distrito Federal ( Gettinger and Gribel 1989); Mato Grosso ( Almeida et al. 2018); Mato Grosso do Sul ( Silva and Graciolli 2013 ; Silva et al. 2017), Minas Gerais (present study); Phyllostomus hastatus (Pallas, 1767) : Minas Gerais and São Paulo ( Confalonieri 1976); Mato Grosso ( Almeida et al. 2018); Mato Grosso do Sul ( Silva and Graciolli 2013 ; Silva et al. 2017); Rio de Janeiro (Almeida t e al. 2011; Almeida et al. 2018 ; Lourenço et al. 2016 ; Lourenço et –

in red arrow; E and F – Sternal plate with sternal setae St (1–St4); G – Proximal anterodorsal ad () seta on femur–tibia I; H – Distal posteroventral

(pv) seta on femur–tibia I (slender) and tarsus I (short, enlarged and peglike), indicated in red arrow; I – Proximal ad anteroand) (posterodorsal (pd) setae on tibia II; J – Proximal ad and pd setae on femur II; K – Distal pv setae on femur II (slender) and genu–tarsus II (short, enlarged and peglike), indicated in red arrow; L – Proximal ad and pd setae on femur IV; M and N – Posterolateral pl () setae on femur–tibia IV with details,

indicated in red arrow. Scale bars: A = 200 µm, C, F and H = 100 µm, B, D, G–N = 50 µm.

al. 2020); Tonatia bidens (Spix, 1823) : Ceará ( Almeida et al. 2016b ; Almeida et al. 2018),

Minas Gerais (present study).

Differential diagnosis — Female: small, idiosoma length up to 779 μm ( Figure 17A );

dorsal opisthosoma with 4-5 pairs of minute setae ( Figure 17D ); sternal plate broadly pear shaped; five pairs of ventral body setae (metasternal setae, genital setae, and three pairs of ventral opisthosomal setae posterior to the sternal plate) expanded basally but with acute tips

( Figure 17E–F ); certain ventral setae of legs I and II short and spinelike to peglike in both sexes.

Ventral setae on trochanters I–II, femora I–II, genua II, and one posteroventral seta of each tarsi

III short, enlarged and peglike. Setae pv on femur-genu IV inflated and bladelike rather than setiform and recurved. Male: sternogenital setae long, with first pair extending well beyond level of first pair of pores and the second through fourth sternogenital setae longer, extending beyond bases of adjacent posterior setae; some setae of ventral intercoxa IV area enlarged and expanded basally; many ventral setae of legs I and II distinctly enlarged, blunt and fusiform;

large dorsal setae of tarsi III–IV coarsely barbed or serrated; proximal anterodorsal ad) seta (

of femur–tibia I and genu IV always small. Nymphs: similar to males with regard to above features, except by ontogenetic differences ( Deunff et al. 2011) ( Figure 19A–N ).

Remarks — This is the first record of P. torrealbai on bats Ph. discolor and T. bidens to Minas Gerais state. This is a stenoxenous species, associated with the phyllostomine bats Phyllostomus and Tonatia , and the smallest species associated with genus Phyllostomus

Lacépède, 1799 (the other are P. acutisternus and P. grandisoma Herrin & Tipton, 1975 ) ( Herrin and Tipton 1975 ; Almeida et al. 2018). Morphological characters match original description and re-descriptions ( Machado-Allison 1965a ; Furman 1966 ; Herrin and Tipton 1975 ; Almeida et al. 2018).

In our bGMYC species delimitation ( Figure 2 ), Periglischrus torrealbai is represented by six haplotypes (1–4 and 6–7) out of six sequences and recovered as two putative species with

pp.>95%: P. torrealbai A (7) from Tonatia bidens and P. torrealbai B (1 to 4 and 6) from

Phyllostomus hastatus and Ph. discolor . This result is in part consistent with results of Almeida et al. (2018), who showed that P. torrealbai morphology varies on a morphometric basis among the host bat species P. discolor , P. hastatus and T. bidens with three morphologically distinct species with host specificity, and suggests that P. torrealbai includes at least two distinct species of Periglischrus , one with Phyllostomus and Tonatia genus.

Figures

Figure 2 Mitochondrial COI haplotype ultrametric tree inferred in BEAST using strict clock and species delimitation probabilities obtained in bGMYC.

Figure 17 Periglischrus torrealbai, female. A – General view; B – Mediodistal lobe of palpal tibia indicated in red arrow; C – Dorsal plate with proteronotal setae (Pn1–Pn5) and poststigmal seta (Pst) indicated; D – Dorsal opisthosoma with hysteronotal setae (Op3–Op6) indicated

Figure 18 Periglischrus torrealbai, male. A – General view; B – Dorsal view with proteronotal setae (Pn1–Pn5) and poststigmal seta (Pst) indicated; C – Ventral view with sternogenital setaeSt(1–St4) and genital seta (Sg) indicated; D – Ventral setae on legs I and II with details; E Details ventral setae on leg I; F – Details ventral setae on leg II; G – Coxa I with pv anddvsetae, indicated in red arrow; H – Femur–tibia I with proximal adsetae, indicated; I – Coxa II with posterolateral setapl () indicated; J – Femur II, proximaladandpdsetae, indicated; K – Proximal adandpdon tarsus III, indicated; L – Femur IV, proximaladandpdsetae, indicated; M – Genu IV, proximaladandpdsetae, indicated; N – Proximal adandpdon tarsus IV, indicated. Scale bars: A = 200 µm, B–D =100 µm, E–N = 50 µm.

Figure 19 Periglischrus torrealbai, protonymph. A – General view; B – Dorsal view; C – Ventral view; D – Ventral setae on legs I and II with details; E – Details ventral setae on leg I; F – Details ventral setae on leg II; G – Coxa I withpv anddvsetae, indicated; H – Femur–tibia I with proximal adsetae, indicated; I – Coxa II with posterolateral setapl () indicated; J – Femur II, proximaladandpdsetae, indicated; K – Proximal adandpdon tarsus III, indicated; L – Femur IV, proximaladandpdsetae, indicated; M – Genu IV, proximaladandpdsetae, indicated; N – Proximal adandpdon tarsus IV, indicated. Scale bars: A = 200 µm, B–D = 100 µm, E–N = 50 µm.