Nonomuraea diastatica, Saygin, Hayrettin, Nouioui, Imen, Ay, Hilal, Guven, Kiymet, Cetin, Demet, Klenk, Hans-Peter, Goodfellow, Michael & Sahin, Nevzat, 2020

Saygin, Hayrettin, Nouioui, Imen, Ay, Hilal, Guven, Kiymet, Cetin, Demet, Klenk, Hans-Peter, Goodfellow, Michael & Sahin, Nevzat, 2020, Polyphasic classification of Nonomuraea strains isolated from the Karakum Desert and description of Nonomuraea deserti sp. nov., Nonomuraea diastatica sp. nov., Nonomuraea longispora sp. nov. and Nonomuraea mesophila sp. nov., International Journal of Systematic and Evolutionary Microbiology 70 (1), pp. 636-647 : 644

DESCRIPTION OF NONOMURAEA DIASTATICA SP. NOV.

Nonomuraea diastatica (di.a.sta ′ ti.ca. N.L. fem. adj. diastatica from eng. n. diastase, a starch-degrading enzyme, derived from Gr. fem. n. diastasis a parting, a separation).

Aerobic, Gram-stain-positive, non-motile actinobacter’um that forms extensively branched substrate and aerial mycelia. Aerial hyphae bear clusters of oval spores. Good growth occurs on ISP 2, modified Bennett’s, Czapek’s, tryptic soy and nutrient agar, moderate growth on ISP 4, 5 and 7 agar and weak growth on ISP 6 agar substrate mycelia may be brown or cream, not produce diffusible or melanoid pigments. Grows from 28–37 °C (optimum, 28 °C), from pH 6.0–8.0 (optimum, pH 7.0) and in the presence of up to 4 % (w/v) NaCl. Hydrolyses aesculin and arbutin, reduces nitrate but does not produce H 2 S and does not hydrolyse allantoin or urea. Gelatin, hypoxanthine, starch and xylan are degraded, but not adenine, casein, chitin, guanine, Tweens 40 or 80 or xanthine. Cellobiose, dextran, D-glucose, D-mannitol, raffinose and sodium succinate are utilized as sole carbon and energy sources, but not adonitol, D-arabinose, L-arabinose, dextrin, D-fructose, D-galactose, L-glutamine, myo-inositol, inulin, lactose, maltose, D-mannose, melezitose, melibiose, L-rhamnose, D-sorbitol, L-sorbose, sucrose, xylitol or D-xylose. L-Alanine, L-arginine, L-asparagine, L-cysteine, glycine, L-hydroxyproline, α-iso-leucine, L-methionine, L-phenylalanine, L-proline, L-serine, L-tyrosine and L-valine are used as sole nitrogen sources, but not l-histidine. The predominant menaquinones are MK-9(H 4) and MK-9(H 6). The polar lipid profile includes diphosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, dihydroxy-phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified glycophospholipids, an unidentified phospholipid and three unidentified lipids. Whole-cell hydrolysates contain meso -A 2 pm, glucose, mannose, madurose, ribose and galactose. The major fatty acids are C 17: 0 10-methyl, iso-C 16:0 and C 16:0. The DNA G+C content of strain KC712 T is 70.3 mol% and the genome size 10.6 Mbp.

The type strain, KC712 T (=CGMCC 4.7334 T =DSM 102925 T =KCTC 39776 T), was isolated from desert soil sample collected from the Karakum Desert , Turkmenistan. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain KC712 T is MG770679 and draħ genome sequence accession number SMKP00000000 .