Cladosporium herbarum

Doyama, Kohei, Haruma, Toshikatsu, Hishiyama, Shojiro, Kato, Atsushi, Masuya, Hayato & Yamaji, Keiko, 2023, Isoavenaciol and 7 - hydroxy-isoavenaciol: Zn-chelating metallophores produced by root-endophytic Pezicula ericae in a Zn-accumulating plant, Aucuba japonica, Phytochemistry (113547) 206, pp. 1-7 : 6

4.8. Evaluation of antifungal activity using Cladosporium herbarum

The antifungal activity against Cladosporium herbarum (AHU9262) was tested using the paper disk method. A water-suspended spore solution of C. herbarum (500 μL) was aseptically added to potato dextrose agar medium at approximately 35 ◦ C (PDA; 2% agar in 10 mL) in a plate. Five different dosages (6.25, 12.5, 25, 50, and 100 μg per disk) in ethyl acetate and the control (ethyl acetate) were aseptically loaded onto paper disks (8 mm i. d.), which were then dried to remove the solvent and placed on the PDA plate. The plate was incubated at 23 ◦ C for 36 h in the dark and the growth inhibition zones at right angles were measured. The results were obtained in triplicate and reported as their average value, and the standard errors were calculated.