Parahelicomyces yunnanensis H.W. Shen, L.L. Li, H.Y. Su & Z.L. Luo, 2022

Li, Long-Li, Shen, Hong-Wei, Bao, Dan-Feng, Lu, Yong-Zhong, Su, Hong-Yan & Luo, Zong-Long, 2022, New species, Parahelicomyces yunnanensis sp. nov. and Tubeufia nigroseptum sp. nov. from freshwater habitats in Yunnan, China, Phytotaxa 530 (1), pp. 21-37 : 28-29

Parahelicomyces yunnanensis H.W. Shen, L.L. Li, H.Y. Su & Z.L. Luo , sp. nov. FIGURE 2

Index Fungorum number: IF 558685 ; Facesoffungi number: FOF 10259 ;

Etymology: —Referring to Yunnan province, China, where the fungus was collected.

Holotype: —KUN-HKAS 115525

Saprobic on decaying wood in a freshwater stream. Asexual morph: Hyphomycetous, helicosporous. Colonies on the substratum superficial, effuse, gregarious, white to pale brown. Mycelium composed of partly immersed, partly superficial, hyaline to pale brown, septate, branched hyphae, with masses of crowded, glistening conidia. Conidiophores macronematous, mononematous, cylindrical, multiple branched, septate, 107–236 µm long, 3–5 µm wide, hyaline to pale brown, many bulges on the nodule, smooth-walled. Conidiogenous cells holoblastic, mono-to polyblastic, integrated, sympodial, terminal or intercalary, cylindrical, has many obvious bulges on the nodule, with denticles, directly from conidiophores near each septum, some of them are arising laterally from the conidiophores as tiny bladder-like protrusions 2–9 µm long, 1–3.5 µm wide, hyaline to dark brown, smooth-walled. Conidia solitary, acropleurogenous, helicoid, rounded at tip, multi-septate, 28–55 µm diam, and conidial filaments 2–3.5 µm wide (x = 39 × 2.5 µm, n = 31), 104–156 µm long, coiled 1–3 times, becoming loosely coiled in water, guttulate, hyaline to pale brown, smooth-walled. Sexual morph: Undetermined.

Material examined:— CHINA, Yunnan province, Dali city, Binchuan county, Jizushan Mountain , on submerged decaying wood in freshwater stream, August 2017, Hong-Wei Shen , S-832 (KUN-HKAS 115525, holotype), ex-type living culture, CGMCC 3.20429 .

Notes: — Parahelicomyces yunnanensis resembles P. hyalosporus in having, branched, septate hyaline to pale brown conidiophores. However, they are different in size of conidia ( P. yunnanensis = 104–156 × 2–3.5 vs. P. hyalosporus =126.5–237 × 4–7 µm). The conidiophores of Parahelicomyces yunnanensis are longer (107–236 vs. 26–53 μm). In addition, our isolate conidia, guttulate, conidiogenous cells has many obvious bulges on the nodule and irregular, with denticulate, hyaline to dark brown. In the phylogenetic analysis, P. yunnanensis is close to P. hyalosporus , following Jeewon & Hyde (2016) for delimitation of new species, nucleotide comparison between P. yunnanensis and P. hyalosporus shows there are 14, 1, 14 and 11 differences in ITS, LSU, RPB 2, and TEF 1-α sequence data respectively, which supports them to be different species. Therefore, Parahelicomyces yunnanensis is introduced as a new species.

Figures

FIGURE 2. Parahelicomyces yunnanensis (KUN-HKAS 115525, holotype). a Colony on decaying wood. b–d Conidiophores with attached conidia. e–f Conidiogenous cells. g–m Conidia. n Germinating conidium. Scale bars: b = 90 μm, c–d =40 μm, e–f = 10 μm, g–m = 20 μm.