Apiospora arundinis (Corda) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 205 (2021)

Li, Shuji, Peng, Cheng, Yuan, Rong & Tian, Chengming, 2023, Morphological and phylogenetic analyses reveal three new species of Apiospora in China, MycoKeys 99, pp. 297-317 : 297

Apiospora arundinis (Corda) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 205 (2021)

Fig. 2

Description.

Asexual morph: Mycelium consisting of smooth, hyaline, branched, septate, 1.1-5.9 µm diam. hyphae (n = 20). Conidiophores reduced to conidiogenous cells. Conidiogenous cells subglobose to ampulliform, erect, blastic, aggregated in clusters on hyphae, smooth, branched, 3.4-9.4 × 1.5-6.4 µm, mean ( ± SD): 6.8 ( ± 1.6) × 3.9 ( ± 1.3) µm (n = 50). Conidia globose, subglobose to lenticular, with a longitudinal germ slit, occasionally elongated to ellipsoidal, brown to dark brown, smooth to finely roughened, 6.4-10.4 × 5.2-8.3 µm, mean ( ± SD): 7.7 ( ± 0.6) × 6.8 ( ± 0.7) µm, L/W = 1.0-1.5 (n = 50). Sexual morph: Undetermined.

Culture characteristics.

On PDA, colonies thick and dense, margin undulate and irregular, pale yellow pigment diffused into medium, surface with patches of iron-grey aerial mycelia, reverse yellowish-brown, mycelia white to grey, sporulation on hyphae, reaching 9 cm in 7 days at 25 °C.

Specimens examined.

China, Yunnan Province: Xishuangbanna Botanical Garden , on diseased leaves of Brunfelsia brasiliensis , 6 June 2022, S.J. Li, BJFC-S1918; living cultures CFCC 58977, LS 107 ).

Notes.

In this study, two isolates clustered together with the culture of A. arundinis with high-support values (ML/BI = 100/0.99)in the multi-locus phylogenetic tree (Fig. 1 ). Thus, these isolates were identified as A. arundinis and Brunfelsia brasiliensis as a new host record for this species. Apiospora arundinis was introduced from Phyllostachys praecox , Castanea mollissima and Saccharum officinarum in China ( Chen et al. 2014; Jiang et al. 2021; Liao et al. 2022). Comparing with the description from Chen et al. (2014) (5-7 × 2-4 µm), Jiang et al. (2021) (3-4 µm) and Liao et al. (2022) (4.5-7.4 × 3.3-4.4 µm), the conidia in this study show larger sizes (6.4-10.4 × 5.2-8.3 µm). These differences may result from different host and habitat.

Figures

Figure 1. Phylogram of Apiospora, based on combined ITS, LSU, tef 1 and tub 2 genes. ML bootstrap support values (≥ 50 %) and Bayesian posterior probability (≥ 0.90) are shown as first and second position above nodes, respectively. Strains from this study are shown in blue boxes, ex-type or ex-epitype cultures are indicated in bold face. Some branches were shortened according to the indicated mulipliers.

Figure 2. Apiospora arundinis (CFCC 58977) A leaf of host plant B colony on PDA C conidiomata formed in culture D, E conidiogenous cells giving rise to conidia F conidia. Scale bars: 1000 µm (C); 10 µm (D-F).