T. neofibulifera Kobayasi, Scientific Report, Tokyo Bunrika Daigaku, Section 4: 15 (1939)

Fan, Long-Fei, Alvarenga, Renato Lucio Mendes, Gibertoni, Tatiana Baptista, Wu, Fang & Dai, Yu-Cheng, 2021, Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota), MycoKeys 82, pp. 33-56 : 33

T. neofibulifera Kobayasi, Scientific Report, Tokyo Bunrika Daigaku, Section 4: 15 (1939)

Figs 3D , 8

Basidioma.

Sessile, when fresh soft gelatinous, creamy-white to pale yellowish, irregularly cerebriform or slightly foliose, with undulate lobes, up to 4.5 cm long, 2.0 cm broad and 2.5 cm high from base, becoming firmly gelatinous and invisible yellowish when dry, broadly attached to substratum.

Internal features.

Hyphae hyaline, smooth thin- to slightly thick-walled, 2.0-6.0 µm in diameter, branched, interwoven, with abundant clamp connections, clamp complexes and anastomoses, slightly thick-walled hyphae usually present near to base of basidioma, sometimes swollen up to 8.5 μm; hyphidia hyaline, smooth, thin-walled, arranged in cluster, usually parallel; vesicles infrequent, thick-walled; swollen cells and haustoria absent; mature basidia thin-walled, ovoid to subglobose, with a basal clamp connection, 14.0-16.0 × 13.0-17.0 μm, L = 14.9 µm, W = 14.8 µm, Q = 1.01 (n = 30/1), sometimes their width greater than length, usually longitudinally septate, rarely obliquely septate, 2-4-celled, with obvious oil drops; sterigmata up to 70 μm long, 1.5-2.0 in diameter, slightly protuberant at apex; probasidia thin-walled, ellipsoid to subglobose, usually proliferating from terminal hyphae; basidiospores hyaline, thin-walled, ellipsoid to broadly ellipsoid, apiculate, with oil drops, 8.0-10.0 × 6.0-8.0 μm, L = 8.9 µm, W = 6.5 µm, Q = 1.37 (n = 30/1), germination by germ tubes or secondary spores; conidia absent.

Specimens examined.

China Jilin, Helong, Quanshuidong Forest Farm, on stump of Quercus , 15 July 2017, F. Wu 243 (BJFC031046); F. Wu 244 (BJFC031047); F. Wu 248 (BJFC031051) .

Notes.

Three specimens listed above from Northeast China together with LE303445 from Far East of Russia formed a distinct lineage closely related to T. guangxiensis in our phylogenies (Figs 1 , 2 ). T. neofibulifera was originally described from Japan ( Kobayasi 1939), and our studied East Asian samples have similar morphology to T. neofibulifera except bigger basidiospores (Table 3 ). We fail to loan the type of T. neofibulifera , and for the time being we treat our studied East Asia samples as T. " Tremella neofibulifera ". The current T. " Tremella neofibulifera " differs from other similar species of the Tremella fibulifera complex by the parallel hyphidia and the presence of vesicles. In addition, T. " Tremella neofibulifera " are different from T. guangxiensis , T. australe , T. subfibulifera and T. fibulifera s.s. by 3.15%, 5.25%, 7.14%, and 8.19% sequence differences in the ITS sequences and 2.04%, 1.32%, 3.18%, and 2.41% in the partial nLSU sequences respectively.

Figures

Figure 1. The Maximum likelihood tree showing phylogenetic relationship of species in Tremella s. s. based on the ITS + partial nLSU dataset. Bootstrap support values for MP and ML greater than 50 % and BI greater than 0.95 are given at each node respectively. The samples used in this study are in bold.

Figure 2. The Maximum likelihood tree showing phylogenetic relationship of species in Tremella s. s. based on the combined ITS + partial nLSU + mtSSU + TEF 1 + RPB 1 + RPB 2 dataset. Bootstrap support values for MP and ML greater than 50 % and BI greater than 0.95 are given at each node respectively. The samples used in this study are in bold.

Figure 3. Basidioma A Tremella fibulifera (Alvarenga 471) B T. australe (Wu 154) C T. guangxiensis (Wu 3) D T. latispora (Dai 17568) E T. " Tremella neofibulifera " (Wu 244) F T. subfibulifera (Alvarenga 334). Scale bars: 1 cm (A-F).

Figure 8. Microscopic structures of Tremella " Tremella neofibulifera " (Wu 248) A basidiospores B germination tubes of basidiospores and secondary spores C-E basidia at different stages F probasidia G hyphae with clamp connections and clamp complexes H vesicles I parallel hyphidia in hymenium. Scale bars: 10 μm (A-I).