Lepidocyrtoides caeruleomaculatus, Bellini, 2017

Bellini, Bruno Cavalcante, 2017, The discovery of Lepidocyrtoides Schött, 1917 (Collembola, Entomobryidae, Entomobryinae) from the New World, including three new species from Brazil and one from Australia, Zootaxa 4324 (2), pp. 201-248 : 202-209

Lepidocyrtoides caeruleomaculatus sp. nov. Cipola & Bellini

FigS 1A , 3–10

Diagnosis. Body with dark blue pigment laterally on head, Th II–III, Abd III–IV, and two bandS on femora I–III ( Fig. 1A ); dorSal head with 3 median and 3 Sutural mac, Pp5 mac abSent; labral papillae reduced or abSent; baSomedian and baSolateral labial fieldS with chaetae M1–2 and R ciliate, otherS Smooth; 4 anterior poStlabial chaetae ciliate and without Spine-like chaetae ( FigS 3C, E , 5D , 6A, C ); Th II with 14–15 anterior mac and 1 poSterior meS (p3); Th III–Abd III with 0, 0, 3 and 1 median mac reSpectively; Abd IV with 13 median mac and 11 lateral mac ( FigS 7 , 8A–B ); trochanteral organ with about 107 Spine-like chaetae; ungueS apical tooth preSent; unguiculuS I truncate, II–III acuminate ( FigS 9D , 10A–C ); manubrial plate with 4 blunt mac; dorSal denS with 2 proximal rowS of unilaterally ciliate chaetae ( FigS 4D–G , 10G ).

Type material. Holotype female on Slide (063/ INPA): Brazil, AmazonaS, Rio Preto da Eva municipality, “ Santa Terezinha ” farm, 02°40’15”S, 59°39’22”W, Amazon foreSt , 45 m, 30.x.2013, SuSpended trap, BG Oliveira coll. ParatypeS on SlideS (063A-O/ INPA): 14 femaleS and 1 juvenile, and 30 SpecimenS in alcohol, Same data aS holotype. ParatypeS in alcohol ( DBEZ / UFRN): 5 SpecimenS, Same data aS holotype.

Additional material. 1 male and 2 femaleS on SlideS and 2 SpecimenS in alcohol (INPA): Brazil, AmazonaS, Rio Preto da Eva municipality, “ApriSco PáSargada” farm, 02°42'26.6"S, 59°42'53.2"W, Amazon foreSt, 44 m, 13.xii.2013, SuSpended trap, BG Oliveira coll. 2 femaleS on SlideS and 20 SpecimenS in alcohol (INPA): PreSidente Figueiredo municipality, road AM 240, km 24, 02°00'46"S, 59°49'43"W, Amazon foreSt, 177 m, 28.vii‒06.viii.2015, MalaiSe trap, AL HenriqueS, F Xavier-Filho & D Carmo coll. 44 SpecimenS in alcohol (INPA): Novo Airão municipality, Stream “Mato GroSSo”, road AM 352, 02°49'00"S, 60°55'08"W, Amazon foreSt, 36 m, 28‒31.iii.2013, yellow plate trap, JT Câmara & AC Maldaner coll. 1 male and 2 femaleS on SlideS and 2 SpecimenS in alcohol (INPA): ManauS municipality, ReServa Ducke, in Grid L3, 02°56’21”S, 59°55’35”W, Amazon foreSt, 102 m, 25‒27.iii.2013, yellow plate trap, NG Cipola & MBC Graça coll. 6 femaleS on Slide and 9 SpecimenS in alcohol (INPA): ReServa Ducke, headquarterS, 02°55’49”S, 59°58’30”W, Amazon foreSt, 75 m, 1‒15.x.2014, MalaiSe trap, AM Silva-Neto coll. 1 Specimen in alcohol (INPA): EStação Experimental de Silvicultura Tropical, road BR 174, km 45, 02°39'19"S, 60°09'24"W, Amazon foreSt, 108 m, 11.ix.1991, Winkler’S extractor, AY Harada coll. 2 SpecimenS in alcohol (INPA): “Embrapa Amazônia Ocidental”, 02°53’42.1”S, 59°59’10.5”W, cultivation of organic Guarana ( Paullinia cupana Kunth , Sapindaceae ), 97 m, 23.xi.2012, Möerick trap, K Schoeninger coll. 1 female on Slide and 2 SpecimenS in alcohol (INPA): ReServa "ZF2", km14, 02°35'21"S, 60°06'55"W, Amazon foreSt, 120 m, 17‒31.viii.2016, MalaiSe trap, JA Rafael & FF Xavier Filho coll. 1 Specimen in alcohol (INPA): INPA campuS I, "BoSque da Ciência", 03°05'54"S, 59°59'09"W, urban foreSt, 73 m, i.1991 ‒ xii.1994, Pitfall-trap, C MartiuS coll. 3 femaleS on SlideS and 39 SpecimenS in alcohol (INPA): MauéS municipality, farm “Santa Helena”, rural road “MoraiS”, 03°27'13"S, 57°39'01"W, Amazon foreSt, 23 m, 25‒28.iv.2017, MalaiSe trap, A Somavilla coll.

Description. Total length (head + trunk) of SpecimenS 3.71–4.38 mm (n=4), holotype 4.38 mm. SpecimenS pale yellow to white with dark blue pigment on diStal and proximal Ant IV, proximal Ant I–III, anterior and lateral head, lateral Th II, Th III laterally with one anterior Spot, Abd III–IV with one and three lateral SpotS reSpectivelly, femora and tibiotarSi with proximal and diStal bandS, except tibiotarSuS III only with proximal band, and manubrium dorSo-proximally; eyepatcheS black ( Fig. 1A ). JuvenileS with Similar color pattern aS adultS. ScaleS preSent on Ant I to proximal Ant IV, dorSal and lateral head, thorax and abdomen dorSally, legS (except empodia), collophore anteriorly, and manubrium and denteS ventrally ( Fig. 4A ).

Head. Antennae Slightly Shorter than trunk length ( Fig. 1A ), ratio antennae: trunk = 1: 1.20–1.22 (n=4), holotype 1: 1.22; antennal SegmentS ratio aS I: II: III: IV = 1: 1.07–1.59: 1.31–1.41: 2.16–2.55, holotype 1: 1.59: 1.36: 2.34. Ant IV annulated on diStal three quarterS, without apical bulb, with S-blunt SenS of different SizeS and ciliate chaetae ( FigS 3A , 5A ). Ant III SenSe organ with 2 rodS, 3 Spiny guard SenSilla (pluS one poSterior), Sblunt SenS and ciliate chaetae ( Fig. 5B ). Ant I dorSally with 2 median mac and 3 Smooth mic at baSe ( Fig. 5C ). Head dorSal chaetotaxy with 15 antennal (An), 4 anterior (A), 3 median (M), 3 Sutural (S), 1 poSt-Sutural (PS) and 2 poStoccipital anterior (Pa) chaetae ( FigS 3C , 5D ). EyeS G and H Smaller, B larger, otherS Subequal, with 8 interocular chaetae and 3 ScaleS ( FigS 3C , 5D–E ). Clypeal formula with 4 (l1–2), 11 (frontal), 3 (pf0–1) ciliate chaetae, l1–2, pf0–1 larger, 4 frontal Smaller, otherS Subequal. Prelabral chaetae Smooth; labrum with chaeta a1 not thick, p0–1 larger ( Fig. 5F ). Labral papillae reduced or abSent. Maxillary palp with Smooth apical appendage (a.a.) and baSal chaeta (b.c.) gently ciliate, thick and 0.82 Smaller in length than the apical; Sublobal plate with tree Smooth appendageS ( Fig. 6B ). Labial palp with five main papillae (A–E) pluS one hypoStomal papilla (H) with 0, 5, 0, 4, 4, 2 guard appendageS, reSpectively, lateral proceSS (l.p.) apically finger-Shaped and not reaching the baSe of the papilla E; labium with five Smooth proximal chaetae (p.c.) ( FigS 3E , 6C ). BaSolateral and baSomedian labial fieldS with chaetae M1–2, R, e, l1–2, M2 and r Smaller ( FigS 3E , 6A, C ). Ventral head with numerouS ciliate chaetae of different SizeS, 4 anterior

poStlabial chaetae; cephalic groove with about 16 Surrounding ciliate chaetae, 4 median mic, 1 larger baSal chaetae (b.c.), otherS Subequal ( Fig. 6A ).

Thorax chaetotaxy ( FigS 4A , 7A–B ). Th II with about 17–18 antero-median chaetae, 14–15 mac and 3 meS; poSteriorly with 1 median meS (p3). Th III with 7 lateral main meS (a7, m5–7, p5–6). Ratio Th II: III = 3.35–2.72: 1 (n=4), holotype 2.75: 1 ( Fig. 4A ).

Abdomen chaetotaxy ( FigS 4B , 7C–D , 8A–C ). Abd I laterally with 2 meS (m6 and p6). Abd II with 3 central mac (a2, m3–3e) and 3 lateral mac (m5–6, p6), bothriotricha a5 and m2 with 8 and 7 acceSSory ScaleS reSpectively. Abd III with 1 central mac (m3) and 3 lateral mac (am6, pm6 and p6), bothriotricha a5, m2 and m5 with 10, 6 and 7 acceSSory ScaleS reSpectively, and 9 lateral meS. Abd IV with 13 median mac, 11 lateral mac, 15 acceSSory ScaleS between bothriotricha T2 and T4, 8 poSterior SenS (as and ps type I, otherS type II), and aproximatelly 30 poSterior meS. Abd V with SerieS ‘a’, ‘m’ and ‘p’ with 4, 3 and 4 mac reSpectively. Ratio Abd III: IV = 1: 7.1 3–9.98 (n= 4), holotype 1: 8.23.

LegS. Subcoxa I (outer Side) with 3 chaetae and 3 pSp; Subcoxa II with an anterior row of 21 chaetae pluS 9 chaetae anteriorlly, poSterior row of 7–9 chaetae and 5 pSp; Subcoxa III with one row of 17 chaetae, numerouS anterior chaetae and 2 poSterior pSp ( FigS 9A–C ). Trochanteral organ with about 107 Spine-like chaetae ( Fig. 9D ). Femora I–III with 2 inner chaetae, 1 proximal acuminate and 1 diStal gently ciliate. TibiotarSi I–III not Subdivided, with 7, 10 and 8 inner chaetae gently ciliate reSpectively; outer Side with 5 chaetae ciliate and apically acuminate ( FigS 9E–G ). UngueS outer Side with a pair of large laterobaSal teeth; inner Side with 4 teeth, 2 paired baSal, 1 unpaired median Subequal to baSal teeth, and 1 minute unpaired apical. UnguiculuS I truncate, II–III acuminate, all with outer edge Serrated, other edgeS Smooth; ratio unguiS: unguiculuS = 1: 0.60. TibiotarSuS III diStally with one inner Smooth chaetae 0.63 Smaller than unguiculuS; outer Side with tenent hair capitate, Smooth and 0.79 Smaller than unguiS outer edge ( FigS 4C , 10A–C ).

Collophore. Anterior Side with 18 ciliate chaetae, 4 mac and 2 meS diStally, and 12 thin chaetae; poSterior Side with about 62 ciliate chaetae, 1 unpaired proximal mac and 2 meS, 3 Small proximal SpineS, 41 thin and 14 Smaller chaetae widely diStributed, and diStally 1 thick Smooth chaeta; lateral flap with about 21 chaetae, 10 Smooth and 11 ciliate ( Fig. 10D–E ).

Furcula. Ventral manubrium with 16 Subapical long ScaleS on one tranSverSe row, about 27 apical ScaleS and 6 diStal ciliate chaetae, 2 inner Slightly Smaller ( Fig. 10F ). Manubrial plate with 4 blunt mac (2 Smaller) gently ciliate and apically bullet-like, 19–21 ciliate chaetae different SizeS and 4–5 pSp. DorSal denS with 2 proximal rowS of unilaterally ciliate chaetae, otherS normal ( FigS 4D–G , 10G ). Mucro bidentate with apical tooth Subequal to proximal tooth, baSal Spine SurpaSSing the apex of the proximal tooth ( FigS 4H , 10H ).

Etymology. ReferS to the blue color SpotS and bandS over the body of the new SpecieS (from Latin: caeruleobluiSh and macula— StainS, SpotS) ( Fig. 1A ).

Remarks. Lepidocyrtoides caeruleomaculatus sp. nov. reSembleS Lepidocyrtoides villasboasi comb. nov. ( Arlé & GuimarãeS, 1981) by body with blue SpotS and bandS, Ant IV annulated and without apical bulb, reduced dorSal chaetotaxy of head and Th III, Abd II–III with 3 and 1 median mac reSpectively; ungueS with apical tooth, unguiculuS I truncate and II–III acuminate, and dorSal denS with proximal rowS of unilaterally ciliate chaetae. However, L. caeruleomaculatus sp. nov. differS from thiS SpecieS by diStinct color pattern with Scattered SpotS, while L. villasboasi comb. nov. haS tranSverSe bandS of dark blue on Th III–Abd III and Abd IV poSteriorly ( FigS 1A, H ). The chaetotaxy of L. caeruleomaculatus sp. nov. differS by abSence of Pp5 mac on head (preSent in L. villasboasi comb. nov.), baSomedian labial field with chaetae e, l1–2 Smooth and R preSent (E, L1–2 ciliate and R abSent in L. villasboasi comb. nov.), and ventral head devoid of Spine-like mic on anterior poStlabial region (preSent in L. villasboasi comb. nov.). Th II differS by 14–15 anterior mac and 1 poSterior meS (22–24 anterior and 5 poSterior mac in L. villasboasi comb. nov.), Abd I without mac (1 in L. villasboasi comb. nov.), and Abd IV with 13 median mac (16–20 in L. villasboasi comb. nov.). Other differenceS of L. caeruleomaculatus sp. nov. are trochanteral organ with about 107 Spine-like chaetae (48 in L. villasboasi comb. nov.); and manubrial plate with 4 blunt mac (2 in L. villasboasi comb. nov.).

Figures

FIGURE 1 A – J. Lepidocyrtoides spp., habitus of specimens fixed in alcohol (lateral view). A, L. caeruleomaculatus sp. nov.; B – D, L. colormutatus sp. nov.: B, adult, C, subadult, D, juvenile; E, L. bicolorangelus sp. nov.; F – G, L. tapuia comb. nov: F, adult, G, juvenile; H, L. villasboasi comb. nov. preserved in slide; I – J, L. hopkini sp. nov.: I, adult, J, juvenile. Scale bars: A – B, F, H – I (1 mm), C – E, J (0.5 mm), G (0.2 mm).

FIGURE 3 A – E. Lepidocyrtoides caeruleomaculatus sp. nov.: head; A, left Ant IV (dorsal side), arrow indicates the beginning of annulations; B, left Ant II distally (lateral side); C, eyepatch and part of dorsal chaetotaxy (left side), M 2 – 4, S 5 – 6 and Pp 5 as mac, Ps 5 as mes; D, head dorsal mac; E, labial palp, proximal chaetae (p. c.), basomedian and basolateral fields (a 5 and l 2 omitted) (right side), arrow indicate lateral process (l. p.) of papilla E. Scale bars: A – B (0.1 mm), C, E (0.02 mm), D (0.01 mm).

FIGURE 4 A – H. Lepidocyrtoides caeruleomaculatus sp. nov.: A, Th II – III segments (lateral view); B, distal tibiotarsus and empodial complex I (posterior view), arrow indicates a outer laterobasal tooth (anterior tooth omitted); C, lateral chaetotaxy of Abd III (left side), am 6, pm 6 and p 6 as mac, m 5 bothriotrichum, ms S-microchaeta, acc. p 6 accessory sens of p 6 chaeta; D, distal manubrium and proximal dens (dorsal view), E, blunt mac gently ciliate and apically bullet-like; F, dens median region (lateral view), arrow indicates row of unilaterally ciliate chaetae; G, dorsal row of unilaterally ciliate chaetae on dens; H, distal dens and mucro (lateral view). Scale bars: A (0.2 mm), D (0.05 mm), B – C, F – H (0.02 mm), E (0.01 mm).

FIGURE 5 A – F. Lepidocyrtoides caeruleomaculatus sp. nov.: dorsal head; A, apex of Ant IV; B, Ant III apical organ (lateral side); C, chaetotaxy of the left Ant I (dorsal side); D, cephalic chaetotaxy; E, eyepatch and part of chaetotaxy (left side); F, chaetotaxy of the clypeus, prelabrum and labrum, arrow indicates chaeta a 1 thick of L. bicolorangelus sp. nov.

FIGURE 6 A – C. Lepidocyrtoides caeruleomaculatus sp. nov.: ventral head; A, anterior postlabial and cephalic groove regions chaetotaxy (left side), lateral chaetae omitted; B, maxillary outer lobe and sublobal plate; C, labial palp, proximal chaetae (p. c.), and basomedian and basolateral fields, l. p. is the lateral process of papilla E.

FIGURE 7 A – D. Lepidocyrtoides caeruleomaculatus sp. nov.: dorsal chaetotaxy; A, Th II; B, Th III; C, Abd I; D, Abd II.

FIGURE 8 A – C. Lepidocyrtoides caeruleomaculatus sp. nov.: dorsal chaetotaxy; A, Abd III; B, Abd IV; C, Abd V.

FIGURE 9 A – G. Lepidocyrtoides caeruleomaculatus sp. nov.: legs chaetotaxy; A, subcoxa I; B, subcoxa II; C, subcoxa III (outer side); D, trochanteral organ (posterior side); E, femur and tibiotarsus I; F, femur and tibiotarsus II; G, femur and tibiotarsus III (anterior side).

FIGURE 10 A – H. Lepidocyrtoides caeruleomaculatus sp. nov.: A, distal tibiotarsus and empodial complex I (posterior view); B, distal tibiotarsus and empodial complex III (posterior view); C, empodial complex III (inner view); D, collophore anterior chaetotaxy; E, collophore posterior and lateral flap chaetotaxy; F, manubrium ventro-distal chaetotaxy; G, distal manubrium and proximal dens (lateral view); H, distal dens and mucro (lateral view).