Allophoma thunbergiae Jun Yuan & Yong Wang bis, 2021

Yuan, Jun, Zeng, Xiang-Yu, Geng, Kun, Wijayawardene, Nalin N., Bhat, Jayarama D., Wu, Shi-Ping, Wang, Yong & Yang, Zai-Fu, 2021, Allophoma species (Pleosporales: Didymellaceae) associated with Thunbergia grandiflora in Guangxi Province, China, Biodiversity Data Journal 9, pp. 63643-63643 : 63643

Allophoma thunbergiae Jun Yuan & Yong Wang bis sp. nov.

Materials

Type status: Holotype. Occurrence: recordedBy: Jun Yuan; occurrenceID: GUCC 2070.7; Taxon: scientificName: Allophoma thunbergiae; order: Pleosporales; family: Didymellaceae; genus: Allophoma; Location: country: China; stateProvince: GuangXi; locality: Nanning City, Guangxi Medicinal Botanical Garden ; verbatimCoordinates: 22°51'N, 108°19'E; Identification: identifiedBy: Jun Yuan; dateIdentified: 2020; Record Level: collectionID: HGUP 2070.7

Description

Pathogenic on the leaf spot of Thunbergia grandiflora . Lesions initially on the upper leaf surface, scattered, distinct, irregular, the maximum length of the spot more than 10-15 mm, the edge of the spots yellow, the centre of necrotic section brown, on the lower leaf surface similar. Sexual morph: Undetermined. Asexual morph (Fig. 1 ): Coelomycetous. Conidiomata pycnidial, mostly solitary or aggregated, subglobose to irregular, dark brown, glabrous, covered with some hyphal outgrowths, produced on the agar surface or (semi-)immersed, ostiolate, (39-)44-200 × (48-)49-230 μm (x̄ = 108.9 × 138.9 μm, n = 20). Ostioles 1-3, with a short neck, slightly papillate or sometimes non-papillate. Pycnidial wall pseudoparenchymatous, composed of oblong to isodiametric cells, 3-4 layered, 14-32 μm thick (x̄ = 20.8 μm, n = 10). Conidiogenous cells phialidic, hyaline, smooth, ampulliform to doliiform, 4.5-7 × 4-5 μm (x̄ = 4.9 × 4.6 μm, n = 10), with a distinct periclinal thickening. Conidia oblong to cylindrical, slightly obovoid, smooth and thin-walled, hyaline, aseptate, 3-5 × 1.5-2.5 μm (x̄ = 3.6 × 2.2 μm, n = 20), with two minutes guttules. Conidial exudates not recorded.

Culture characteristics: Colonies on PDA, 46-57 mm diameter after 1 week, irregular at margin, aerial mycelia floccose, grey with a white margin, brown near the centre; reverse pale brown, with a white margin. Colonies on MEA 44-47 mm diameter after 1 week, regular at margin, covered by brown, dense aerial mycelia, yellow near the centre; reverse greyish-brown. Colonies on OA, 41-46 mm diameter after 1 week, irregular at margin, covered by white aerial mycelia sparse, brownish, reverse buff to yellowish-olivaceous.

Etymology

In reference to the host ( Thunbergia grandiflora ), from which the fungus was isolated.

Figures

Figure 1. Allophoma thunbergiae (GUCC 2070.7) a. Leaf symptoms on the host; b, c. Pycnidia forming on PDA; d, e. Colony on PDA (front and reverse); f, g. Colony on MEA (front and reverse); h, i. Colony on OA (front and reverse); j, k. Section of pycnidium; l. Section of pycnidial wall; m, n. Conidiogenous cells; o. Conidia. Scale bars: b, c = 500 μm; j = 100 μm; k = 50 μm; l = 20 μm; m-o = 5 μm.