Graphilbum puerense R.Chang & Z.W.de Beer

Chang, Runlei, Duong, Tuan A., Taerum, Stephen J., Wingfield, Michael J., Zhou, Xudong & Beer, Z. Wilhelm de, 2017, Ophiostomatoid fungi associated with conifer-infesting beetles and their phoretic mites in Yunnan, China, MycoKeys 28, pp. 19-64 : 32-35

Graphilbum puerense R.Chang & Z.W.de Beer sp. nov. Fig. 10

Etymology.

The epithet puerense refers to the city from which this species was collected.

Description.

Sexual state not observed. Pesotum -like macronematal asexual states predominant. Synnemata simple, dark brown at the base, (187.5-) 206- 357(-437.5) μm long including conidiogenous apparatus, (12-) 15.5-45 (-61) μm wide at base; conidiogenous cells (15.5-) 18.5-30.5 (-34) μm long, conidia hyaline, 1-celled, smooth, oblong, (4-) 4-5 (-5.5) × (1-) 1.5-2 (-2.5) μm. Hyalorhinocladiella-like asexual state: conidiophores (17-) 3-140 (-232.5) μm long; cells arising directly from the hyphae, (6.5-) 10-25.5 (-42.5) × (1-) 1-2 (-3) μm; conidia hyaline, smooth, obovoid to oblong, (3.5-) 4-8 (-12) × (1-) 1.5-2.5 (-3) μm.

Culture characteristics.

Colonies hyaline. Mycelium superficial on the 3% OA. Colony margin smooth. Colonies on 2% MEA flat, reaching 76 mm diam in 5 d at 30 °C. No growth observed at 5 °C. Optimal temperature for growth 30 °C.

Type material.

CHINA, Yunnan Province, Puer City, from Polygraphus szemaoensis gallery on Pinus kesiya bark, 29 Jun. 2010, S.J.Taerum, herbarium specimen of dried culture, PREM 61543 (holotype), CMW41673 = CBS139640 (ex-holotype culture).

Additional specimens examined.

CHINA, Yunnan Province, Puer City, from Ips acuminatus gallery on Pinus kesiya bark, 4 Jul. 2010, S.J.Taerum, PREM 61544, CMW41667 = CBS139651; CHINA, Yunnan Province, Puer City, from Ips acuminatus gallery on Pinus kesiya bark, Jul 2010, S.J.Taerum, CMW41942 = CBS139650.

Host.

Pinus kesiya .

Beetle vectors.

Ips acuminatus , Polygraphus szemaoensis .

Mite vectors.

Proctolaelaps nr. hystrix (phoretic on Ips acuminatus ), Insectolaelaps sp. 1 (phoretic on Ips acuminatus ), and Uropodoidea sp. 2 (phoretic on Polygraphus szemaoensis ).

Distribution.

At present known only from Yunnan, China.

Notes.

See comparison between Gra. kesiyae and Gra. puerense above under notes of Gra. kesiyae .