Mycetophila ichneumonea Say, 1823

Juergenstein, Siiri, Kurina, Olavi & Poldmaa, Kadri, 2015, The Mycetophilaruficollis Meigen (Diptera, Mycetophilidae) group in Europe: elucidating species delimitation with COI and ITS 2 sequence data, ZooKeys 508, pp. 15-51 : 25-26

Taxon classification Animalia Diptera Mycetophilidae

Mycetophila ichneumonea Say, 1823 Figs 1, 2, 3, 9, 19, 20, 37, 38

Material.

FINLAND. 1♂, Sodankylä, Syväkuru, 67°25'N, 026°35'E, 21.viii.2013, sweeping, J. Salmela leg. (IZBE0200150, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ434). ESTONIA. 1♂, Tartu county, Melliste, 58°20'N, 26°59'E, 20. viii– 4.ix.2008, Malaise trap, O. Kurina leg. (IZBE0200073, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ272); 1♂, same as earlier except 24. x– 16.xi.2008 (IZBE0200074, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ226); 1♂, Saare county, Orissaare, 58°33'19"N, 23°05'12"E, 18. x– 5.xi.2008, Malaise trap, H. Jäe leg. (IZBE0200075, in alcohol, abdomen used for DNA sequence: SJ239); 1♂, Tartu county, Maiorg near Annikoru, 58°16'41,6"N, 26°20'03,6"E, 1. v– 16.v.2009, Malaise trap, O. Kurina leg. (IZBE0200076, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ129); 1♂, Jõgeva county, Kaiu, 58°39'20,4"N, 26°52'43,2"E, reared from Cortinarius traganus , coll. 21.ix.2011, emerg. 24.x.2011, S. Jürgenstein leg. (IZBE0200077, in alcohol); 1♂, Tartu county, Järvselja, 58°17'45"N, 27°15'41,7"E, reared from Lactarius helvus , coll. 23.ix.2011, emerg. 12.x.2011, S. Jürgenstein leg. (IZBE0200078, in alcohol, abdomen used for DNA sequence: SJ261); 1♂, Tartu county, Järvselja, 58°17'45"N, 27°15'41,7"E, reared from Lactarius torminosus , coll. 23.ix.2011, emerg. 10.x.2011, S. Jürgenstein leg. (IZBE0200079, in alcohol, abdomen used for DNA sequence: SJ223); 1♂, Põlva county, Viira, 58°0'N, 27°11'E, reared from Cortinarius hemitrichus , coll. 10.x.2012, emerg. 31.x.2012, S. Jürgenstein leg. (IZBE0200081, in alcohol, abdomen used for DNA sequence: SJ335); 1♂, Jõgeva county, Kaiu, 58°39'20,4"N, 26°52'43,2"E, reared from Cortinarius armeniacus , coll. 1.x.2012, emerg. 29.x.2012, S. Jürgenstein leg. (IZBE0200082, in alcohol, abdomen used for DNA sequence: SJ337); 1♂, Jõgeva county, Kaiu, 58°39'20,4"N, 26°52'43,2"E, reared from Cortinarius depressus , coll. 1.x.2012, emerg. 31.x.2012, S. Jürgenstein leg. (IZBE0200083, in alcohol, abdomen used for DNA sequence: SJ338); 1♂, Lääne county, Haapsalu, 58°57'N, 23°32'E, reared from Cortinarius saturninus , coll. 25.ix.2012, emerg. 22.x.2012, S. Jürgenstein leg. (IZBE0200085, in alcohol, abdomen used for DNA sequence: SJ342); 1♂, Võru county, Rõuge, 57°44'N, 26°55'E, reared from Cortinarius subgenus Telamonia , coll. 3.ix.2012, emerg. 21.ix.2012, S. Jürgenstein leg. (IZBE0200086, in alcohol, abdomen used for DNA sequence: SJ341); 1♂, Tartu county, Uniküla, 58°16'N, 26°55'E, reared from Cortinarius dolabratus , coll. 2.x.2012, emerg. 2.xi.2012, S. Jürgenstein leg. (IZBE0200087, in alcohol, abdomen used for DNA sequence: SJ345); 1♂, Jõgeva county, Kõduküla, 58°34'N, 26°31'E, reared from Lactarius deterrimus , coll. 15.ix.2012, emerg. 2.x.2012, S. Jürgenstein leg. (IZBE0200088, in alcohol, abdomen used for DNA sequence: SJ346); 1♂, Valga county, Miti, 58°06'16,7"N, 26°22'28,9"E, reared from Lactarius rufus , coll. 14.ix.2011, emerg. 3.x.2011, S. Jürgenstein leg. (IZBE0200089, in alcohol with terminalia in glycerine); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius rufus , coll. 16.ix.2011, emerg. 12.x.2011, S. Jürgenstein leg. (IZBE0200090, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius rufus , coll. 16.ix.2011, emerg. 3.x.2011, S. Jürgenstein leg. (IZBE0200091, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius rufus , coll. 16.ix.2011, emerg. 6.x.2011, S. Jürgenstein leg. (IZBE0200152, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius camphoratus , coll. 16.ix.2011, emerg. 5.x.2011, S. Jürgenstein leg. (IZBE0200092, in alcohol); 1♂, Põlva county, Ihamaru, 58°06'00,40"N, 26°55'55,45"E, reared from Lactarius rufus , coll. 18.ix.2011, emerg. 21.x.2011, S. Jürgenstein leg. (IZBE0200093, in alcohol); 1♂, Põlva county, Ihamaru, 58°06'00,40"N, 26°55'55, 45"E, reared from Rhodocollybia butyracea , coll. 18.ix.2011, emerg. 5.x.2011, S. Jürgenstein leg. (IZBE0200094, in alcohol); 1♂, Jõgeva county, Kaiu, 58°39'20,4 ’’ N, 26°52'43,2"E, reared from Cortinarius sp., coll. 21.ix.2011, emerg. 17.x.2011, S. Jürgenstein leg. (IZBE0200095, in alcohol); 1♂, Jõgeva county, Kaiu, 58°39'20,4"N, 26°52'43,2"E, reared from Cortinarius alborufescens , coll. 21.ix.2011, emerg. 5.x.2011, S. Jürgenstein leg. (IZBE0200096, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius helvus , coll. 16.ix.2011, emerg. 6.x.2011, S. Jürgenstein leg. (IZBE0200097, in alcohol); 1♂, Jõgeva county, Kaiu, 58°39'20,4"N, 26°52'43,2"E, reared from Cortinarius sp., coll. 21.ix.2011, emerg. 12.x.2011, S. Jürgenstein leg. (IZBE0200098, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius helvus , coll. 16.ix.2011, emerg. 12.x.2011, S. Jürgenstein leg. (IZBE0200099, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius rufus , coll. 16.ix.2011, emerg. 10.x.2011, S. Jürgenstein leg. (IZBE0200101, in alcohol with terminalia in glycerine); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius rufus , coll. 16.ix.2011, emerg. 6.x.2011, S. Jürgenstein leg. (IZBE0200102, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius rufus , coll. 16.ix.2011, emerg. 12.x.2011, S. Jürgenstein leg. (IZBE0200103, in alcohol); 1♂, Võru county, Rõuge, 57°44'N, 26°55'E, reared from Cortinarius alborufesens , coll. 3.ix.2012, emerg. 24.ix.2012, S. Jürgenstein leg. (IZBE0200104, in alcohol, abdomen used for DNA sequence: SJ344); 1♂, Lääne county, Haapsalu, 58°57'N, 23°32'E, reared from Cortinarius cotoneus , coll. 25.ix.2012, emerg. 17.x.2012, S. Jürgenstein leg. (IZBE0200105, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ343); 1♂, Lääne county, Vormsi 59°0'N, 23°15'E, reared from Russula vinosa , coll. 25.viii.1991, emerg. 7. ix.1991, O. Kurina leg. (IZBE0200106, pinned); 1♂, Pärnu county, Nigula NR, 58°0'41"N, 24°40'60"E, reared from Megacollybia platyphylla , coll. 5.viii.1990, emerg. 17.viii.1990, O. Kurina leg. (IZBE0200107, pinned); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius helvus , coll. 16.ix.2011, emerg. 12.x.2011, S. Jürgenstein leg. (IZBE0200108, in alcohol); 1♂, Põlva county, Ihamaru, 58°06'00,40"N, 26°55'55,45"E, reared from Lactarius rufus , coll. 18.ix.2011, emerg. 17.x.2011, S. Jürgenstein leg. (IZBE0200109, in alcohol); 1♂, Valga county, Soontaga, 58°01'42,6"N, 26°04'29,3"E, reared from Lactarius helvus , coll. 16.ix.2011, emerg. 5.x.2011, S. Jürgenstein leg. (IZBE0200033, in alcohol, abdomen used for DNA sequence: SJ147; published earlier by Põldmaa et al. 2015); 1♂, Põlva county, Ihamaru, 58°06'00,40"N, 26°55'55, 45"E, reared from Lactarius camphoratus , coll. 18.ix.2011, emerg. 19.x.2011, S. Jürgenstein leg. (IZBE0200156, in alcohol, abdomen used for DNA sequence: SJ138); 1♂, Tartu county, Järvselja, 58°17'45"N, 27°15'41,7"E, reared from Cortinarius brunneus , coll. 23.ix.2011, emerg. 10.x.2011, S. Jürgenstein leg. (IZBE0200157, in alcohol, abdomen used for DNA sequence: SJ148). SLOVAKIA. 1♂, NP Slovensky kras, Silickà Ladnica, 48°33'00,0"N, 020°30'14,4"E, 505m, 3.vi.2009, sweeping, O. Kurina leg. (IZBE0200111, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ318). GEORGIA. 1♂, Surami, 42°01'34,2"N, 43°29'52,5"E, 941m, 18.v.2012, sweeping, O. Kurina leg. (IZBE0200112, in alcohol with terminalia in glycerine, abdomen used for DNA sequence: SJ66); 1♂, Bakuriani, 41°45'46,2"N, 43°30'16,7"E, 1626m, 31.viii.2014, sweeping, O. Kurina leg. (IZBE0200242, slide mounted in Euparal); 1♂, same as earlier except 1.ix.2014 (IZBE0200243, in alcohol); 3♂♂, Bakuriani 1.2 km-W, 41°44'13,0"N, 43°30'45,1"E, 1741m, 1.ix.2014, sweeping, O. Kurina leg. (IZBE0200244-IZBE0200246, in alcohol).

Male terminalia.

Posterior margin of gonocoxite ventromedially with shallow convexity, and with abrupt and blunt projections laterally. Posterior impression with narrow base and well widened posterior part. Anterior impression with anteriorly evenly divergent narrow arms. Ventral branch of gonostylus with symmetrical semi-oval posterior process, which bears minute warts and few setae on ventral surface; spine 1 wide, sharply pointed; spine 2 similar or somewhat longer but more slender; spines 3 and 4 about twice as small, evenly tapering and with about equal distance between each other and spine 2. Ventral branch of gonostylus with 4-5 strong bristles deviating from other setosity laterally on ventral surface. Dorsal branch of gonostylus steeply tapering; lateral margin with deep concavity; proximal posterior process about twice as high as distal posterior process, both separated by deep concavity. Distal posterior process with apical small setula and with a basal strong seta deviating from other setosity; proximal posterior process apically rounded with 1-2 strong basal setae deviating from other setosity. Posterior margin proximally from medial bristle with 4-5 gradually diminishing bristles followed by 3-4 smaller setae; internal surface with a stronger seta next to the medial bristle. Basal angle clearly outlined, angular or somewhat rounded; basal margin with few setae. Ejaculatory apodeme with campanulate base and without rim. Aedeagus oval or cross shaped. Aedeagal guides extending over apical part of aedeagus; apically rounded and divided into two lamellae; with lateral impressions very small. Aedeagal apodemes laterally slightly angular or arched and pointed apically.

Intraspecific variation.

In some cases the lateral margin of the dorsal branch of the gonostylus is shallower, and the distal posterior process and the posterior margin of the dorsal branch of the gonostylus resemble those of Mycetophila uninotata . The spine 2 on the ventral branch of the gonostylus compared to the spine 1 can be more prominent than described by Laštovka (1972).

Hosts and distribution.

Known from many species of Agaricales and Russulales ( Kurina 1991, Yakovlev 1994, Chandler 2010, Ševčík 2010). Our material from pine dominated boreal forests in Estonia indicates specialisation to Lactarius ( Russulales ) and Cortinarius ( Agaricales ). Widely distributed in Europe extending also to the Eastern Palaearctic, the Middle East and the Nearctic region ( Laštovka 1972, Chandler 2013). The material from Georgia represents a new country record.