Ceratocystiopsis yantaiensis R.L. Chang & X.Y. Zhang, 2021

Chang, Runlei, Zhang, Xiuyu, Si, Hongli, Zhao, Guoyan, Yuan, Xiaowen, Liu, Tengteng, Bose, Tanay & Dai, Meixue, 2021, Ophiostomatoid species associated with pine trees (Pinus spp.) infested by Cryphalus piceae from eastern China, including five new species, MycoKeys 83, pp. 181-208 : 181

1. Ceratocystiopsis yantaiensis R.L. Chang & X.Y. Zhang sp. nov.

Fig. 9

Holotype.

China. Shandong province: Kunyushan National Forest Park, Yantai city, from the gallery of Cryphalus piceae on Pinus thunbergii , 2 Sep. 2020, R. L. Chang (HMAS249924-holotype; SNM650 = CGMCC3.20247 - ex-holotype culture).

Additional cultures checked.

China. Shandong province: Kunyushan National Forest Park, Yantai city, from the gallery of Cryphalus piceae on Pinus thunbergii , 2 Sep. 2020, R. L. Chang (SNM582).

Etymology.

The name refers to Yantai City, where this fungus was isolated.

Diagnosis.

Ceratocystiopsis yantaiensis differs from closely related species by the production of smaller conidia.

Description.

Sexual morph is unknown. Asexual state hyalorhinocladiella-like: the conidiophores directly arising singly from the vegetative hyphae, measuring (2.4-) 4.7-26.7 (-46.4) μm × (0.8-) 1.0-1.5 (-1.8) μm (Fig. 9d, e ); or a short basal cell which continues to develop short lateral and terminal extensions from conidiogenous sites at their apices or discrete basal cells that produce 1-5 branches, which then branch irregularly and form conidiogenous cells at their apices, measuring (12.2-) 6.2-10.2 (-50.7) μm long (Fig. 9b, c ); conidiogenous cells measuring (4.7-) 6.2-10.2 (-12.4) × (0.7-) 0.9-1.3(-1.5) μm (Fig. 9b, c ); conidia hyaline, smooth, unicellular, short oblong, with rounded ends, measuring (1.1-) 1.4-2.2 (-2.7) × (0.8-) 0.9-1.2 (-1.5) μm (Fig. 9b-e ).

Culture characteristics.

The Colonies are light brown in color on MEA (Fig. 9a ). Mycelia are white, superficially growing on the agar. The optimal temperature for growth was 30-35 °C, reaching 43.0 mm diam in 10 days. No growth was observed at 5 °C.

Distribution.

Currently known from Yantai City in Shandong Province, China.

Note.

Ceratocystiopsis yantaiensis is phylogenetically close to C. manitobensis , but formed a distinct clade on both ITS, LSU, and BT trees (Figs 1 and 2 ). Two types of hyalorhinocladiella-like asexual state were also observed in C. manitobensis ( Hausner et al. 2003). Conidia of C. yantaiensis and C. manitobensis are similar in morphology, but there is a difference in size (1.1-2.7 × 0.8-1.5 vs. 3.0-5.5 × 1.0-2.0 µm, Fig. 9b-e ).

Figures

Figure 1. Maximum likelihood phylogeny of Ceratocystiopsis using complete ITS and partial BT gene regions. The isolates recovered in this study are highlighted in color and in bold font. ML and MP bootstrap support values ≥ 75 are indicated at the nodes as ML / MP. Bold branches indicate posterior probabilities values ≥ 0.9. T indicates ex-type cultures.

Figure 2. Maximum likelihood phylogeny of Ceratocystiopsis using partial LSU gene regions. The isolates recovered in this study are highlighted in color and in bold font. ML and MP bootstrap support values ≥ 75 are indicated at the nodes as ML / MP. Bold branches indicate posterior probabilities values ≥ 0.9. T indicates ex-type cultures.

Figure 9. Morphological characters of asexual structures of Ceratocystiopsis yantaiensis sp. nov. a fourteen-day-old culture on MEA b, c type 1 conidiophores and conidia d-e type 2 conidiophores and conidia. - Scale bars: 10 μm.