Distoseptispora bambusae Y.R. Sun, I.D. Goonasekara, Yong. Wang bis & K. D. Hyde, Biodiversity Data Journal 8(e53678): 6 (2020)

Shen, Hong-Wei, Bao, Dan-Feng, Boonmee, Saranyaphat, Lu, Yong-Zhong, Su, Xi-Jun, Li, Yun-Xia & Luo, Zong-Long, 2024, Diversity of Distoseptispora (Distoseptisporaceae) taxa on submerged decaying wood from the Red River in Yunnan, China, MycoKeys 102, pp. 1-28 : 1

Distoseptispora bambusae Y.R. Sun, I.D. Goonasekara, Yong. Wang bis & K. D. Hyde, Biodiversity Data Journal 8(e53678): 6 (2020)

Fig. 2

Description.

Saprobic on submerged decaying wood in a freshwater stream. Anamorph: Colonies on wood effuse, hairy, dark brown, glistening, solitary or in small group. Mycelium immersed, composed of septate, pale brown to brown hyphae, smooth-walled. Conidiophores (42-)66-103(-115) × 5-6 µm (x̄ = 84 × 6 µm, n = 20), macronematous, mononematous, solitary or in groups, erect, straight or slightly flexuous, unbranched, cylindrical, 4-6-septate, brown, rounded at the apex, slightly enlarged at the base, mooth and thin-walled. Conidiogenous cells (10-)15-22(-25) × 5-6 µm (x̄ = 19 × 5 µm, n = 20), monoblastic, terminal, determinate, subcylindrical, brown, smooth-walled. Conidia (55-)69-126(-168) × 10-12 µm (x̄ = 98 × 11 µm, n = 25), acrogenous, solitary, obclavate, rostrate, olivaceous to pale or dark brown, truncate at base, tapering towards the apex, straight or slightly curved, 7-18-euseptate, constricted at the septa, guttulate, verrucose, thick-walled. Teleomorph: Undetermined.

Culture characteristics.

Conidia germinating on PDA within 12 hrs and germ tubes produced from apex and septa of conidium. Colonies growing on PDA reaching 3-4 cm in one month at 26 °C in the dark, flocculent, fluffy, soft white to light brown mycelium from above, dark brown in the middle, light brown at the edges from below.

Material examined.

China, Yunnan Province, Dali City, Weishan Yi and Hui Autonomous County, 25°29′31"N, 100°06′56"E, on submerged decaying branches in a freshwater stream, 19 February 2022, Z.Q. Zhang & Q.X. Yang YJ 14-24-1 (HKAS 125826, living culture KUNCC 22-12668) .

Notes.

Phylogenetic analysis showed that our new strain KUNCC 22-12668 clusters with the type strain of Distoseptispora bambusae (MFLUCC 14-0583) with 100% ML/1.00 PP support (Fig. 1 ). Furthermore, our new collection (Fig. 2 ) exhibits morphological characters identical to those of the type strain Distoseptispora bambusae (MFLUCC 14-0583). However, our collection has longer conidiophores and conidia. This observation aligns with Yang et al. (2018), suggesting that factors such as incubation time and habitat may influence the lengths of conidiophores and conidia. A comparison of the ITS, LSU, tef 1-α and rpb 2 sequences between our new strain KUNCC 22-12668 and the type strain (MFLUCC 14-0583) reveals only minimal base pair differences. Therefore, based on morphological evidence and phylogenetic affinity, our new strain KUNCC 22-12668 is identified as Distoseptispora bambusae and it is reported from freshwater habitat for the first time in Yunnan, China.

Figures

Figure 1. Maximum likelihood (ML) tree is based on combined LSU, ITS, tef 1 - α and rpb 2 sequence data. Bootstrap support values with a ML greater than 65 % and Bayesian posterior probabilities (PP) greater than 0.95 are given above the nodes, shown as " ML / PP ". The tree is rooted to Aquapteridospora fusiformis (MFLUCC 18 - 1606) and A. lignicola (MFLUCC 15 - 0377). New species are indicated in blue and type strains are in bold.

Figure 2. Distoseptispora bambusae (HKAS 125826) a, b colonies on woody substrates c-e conidiophores f, g conidiogenous cells h-m conidia n germinated conidium o culture on PDA. Scale bars: 50 μm (c-e); 10 μm (f, g); 20 μm (h-n).