Xanthagaricus punjabensis Haqnawaz, Niazi & Khalid, 2023

Haqnawaz, Muhammad, Niazi, Abdul Rehman & Khalid, Abdul Nasir, 2023, Two new species of Xanthagaricus from Punjab, Pakistan, Phytotaxa 583 (2), pp. 163-173 : 168

Xanthagaricus punjabensis Haqnawaz, Niazi & Khalid sp. nov. ( Figs. 1 , 2 )

MycoBank MB843788

Etymology:—Species name punjabensis (Latin) refers to the locality “ Punjab ” where the type specimen was collected.

Diagnosis:—Umbonate disc, presence of veil, dull orange to light yellow lamellae, gray rhizomorphic mycelial mat at the base of stipe, presence of annulus, globose to subglobose basidiospore, narrowly utriform to utriform cheilocystidia, broadly clavate ending elements in pileipellis, clavate to broadly clavate caulocystidia.

Holotype:— Pakistan. Punjab, Muzaffargarh District, Indus Riverbed, near Safari Park Kot , Addu , (30°14’44” N, 70°51’04”E, 134 m asl.) on leaf litter soil under Dalbergia sissoo , rich in organic matter, Aug. 18, 2021, Muhammad Haqnawaz , ( LAH37124 ), GenBank ON372612 ( ITS), ON372606 (28 S).

Description:— Basidiomes, terrestrial, small, gregarious. Pileus 12–22 mm, convex to plano-convex, with the umbonate disc; at young stage veil covered the whole pileus, at mature stage restricted to the disc in the form of one or more large squamules dull reddish brown (7.5 R 5/3), on a dull yellow-orange (10 YR 7/4) background; margins incurved with appendiculate velar remnants. Lamellae free, dull orange (7.5 YR 7/4) to light yellow (7.5 YR 8/3), with a fimbriate edge, sub-distant with 0–1 tier of lamellulae. Stipe 17–23× 1–2 mm, central, terete to flexuous, surface squamulose, squamules dull yellow-orange (10 YR 7/4), central, rough surface, context hollow, at the base with grayish rhizomorphic mycelial mat. Annulus fibrillose, with scars and velar remnants.

Basidiospores [50/2/2] (4.8–)5.1–5.9 (–8.1) × (3.9–)4.1–5.5(–5.8) µm, av. L × av. W = 5.5 × 4.9 µm, Q = 1.0– 1.16, Q av = 1.08, globose to subglobose, pinkish to reddish, with light green guttule and hilar appendage in water, inamyloid in Melzer’s Reagent, thick-walled. Basidia (15.9–)16.0–19.3(–18.6) × (6.6–)6.9–8.5 (–9.6) µm, clavate to broadly clavate, hyaline olivaceous in 5% KOH, thick-walled, clamp connections absent, 4 spored, with greenish oil pigments in 5% KOH. Cheilocystidia (12.9–)15.9–21.1(–21.2) × (4.5–)5.6–8.9(–10.2) µm, narrowly utriform to utriform, hyaline olivaceous in 5% KOH, thin-walled, Pleurocystidia absent. Caulocystidia (14.3–) 15.4–25.6 (–31.1) × (5.9–) 6.2–11 (–12.1) µm, clavate to broadly clavate, hyaline to the olivaceous and olivaceous gray wall in 5% KOH, thick-walled, nonguttulate. Pileipellis irregular epithelioid, upper layer consists of broadly clavate cells 11.6–24.3 × 6.6–12.3 µm, a lower layer made up of globose to subglobose, broadly clavate cells, hyaline olivaceous and olivaceous gray wall, thick-walled, smooth and clamp connections absent. Stipe covering hyphae 3.0–3.7 µm wide, avw = 6.8 µm, irregular trichoderm olivaceous hyaline to greenish, thin-walled, septate, branched, regular, clamp connection absent.

Material examined:— Pakistan. Punjab, Muzaffargarh District, Indus Riverbed , near Safari Park , Kot Adu , (30°14‘44“N, 70°51‘04“E, 134 m asl.) on soil, rich in organic matter, Aug. 11, 2021, Muhammad Haqnawaz, ( LAH37125 ) . GenBank ON372609 ( ITS) & ON372607 (28 S) .

Figures

FIGURE 1. A–C Basidiomata of Xanthagaricus punjabensis. A= Holotype, B= lamellae, C= rhizomorphic mycelial D–E Basidiomata of Xanthagaricus kotadduensis. Holotype. Scale bars: A–E = 5.8 mm, 5 mm, 8.8 mm, 7.8 mm, 8.9 mm. Photos by: Muhammad Haqnawaz.

FIGURE 2.A–E. Microscopic structures of Xanthagaricus punjabensis (HQ–17) (Holotype.LAH37124) A. Basidia, B. Basidiospores, C. Cheilocystidia, D. Caulocystidia, E. pileal elements, Scale bars: A–E = 5.6 µm, 6.8 µm, 6.4 µm, 4.7 µm, 6.5 µm. Photos by: Muhammad Haqnawaz