Polydiscia deuterosminthurus

Baquero, Enrique, Moraza, Maria Lourdes & Jordana, Rafael, 2003, A new species of Polydiscia (Acari, Prostigmata, Tanaupodidae) with reference to its host: a new species of Deuterosminthurus (Collembola, Symphypleona, Bourletiellidae), Zootaxa 188, pp. 1-16 : 9-15

Deuterosminthurus bisetosus sp. nov. ( Figs 14 ­42)

Type­locality: SPAIN, Navarra, Otazu, (UTM co­ordinates 30TWN993375).

Type­specimens: Holotype (female) and allotype (male) ex Genista hispanica L. MZNA00309­01, 26.V.1999, E. Baquero and L. Hernández leg.; paratypes: two specimens on slide MZNA00309­02, eight specimens on SEM stub and six in ethyl alcohol.

Additional material: SPAIN, Navarra, Otazu,: MZNA 00310 (28.V.1999, 3 on slide, 12 on SEM stub, 32 in ethyl alcohol), MZNA 00353 (9.V. 2000, 31 in ethyl alcohol), MZNA 00362 (26.V. 2000, 69 in ethyl alcohol), MZNA 00365 (8.VI. 2000, 4 in ethyl alcohol), MZNA 00389 (30.V.2001, 2 on SEM stub, 26 in ethyl alcohol), E. Baquero and R. Jordana leg. Vedado de Eguaras, (R. Jordana and E. Baquero leg.): MZNA 00349 (8.V.2000, 2 on slide, 2 on SEM stub, 15 in ethyl alcohol). El Yugo, (R. Jordana and E. Baquero leg.): MZNA 00390 (6.VI. 2001, 3 in ethyl alcohol). Huesca, Canal Roya, Pyrenees (30TYN0742, R. Jordana leg.): MZNA 00395 (1.7.2001, 11 in ethyl alcohol). Astún, Pyrenees (30TYN0441, R. Jordana leg.): MZNA 00396 (2.7.2001, 27 in ethyl alcohol), MZNA 00400 (10.7.2001, 13 in ethyl alcohol). Málaga, Benalmádena, (30 SUF 5951, A. Altuna leg.): MZNA 00408, MZNA 00410 and MZNA 00412 (17.VII. 2001, 108 in ethyl alcohol). FRANCE, Somport, Pyrenees (30TXN9843, R. Jordana leg.): MZNA 00394 (29.6.2001, 1 in ethyl alcohol).

Material deposited: MZUN (Museum of Zoology, University of Navarra).

Description ( Figures 1 , 14 ­42). Adults size. Female: 0.75­1.4 mm (n = 4); male: 0.85 mm ( Figure 14 ).

Colour. Live specimens show a pale yellowish general appearance with brown­violet patches around the trichobothria insertions (A, B and C) as the most characteristic feature. They are variable from pale brown­violet to red patches over the great abdomen ( Figures 36 ­42). Specimens of this species collected from Pyrenees, Bardenas Reales (Southern Navarra) (Figure 40) and Málaga (Southern Spain) (Figure 41) show a great variation of the pattern. This pattern includes specimens which are completely yellow (Otazu) (Figures 39 and 42) to specimens with a characteristic pattern with a yellow ‘V’ shaped form over the dark red on the last half of the great abdomen ( Figure 38 , 40 and 41). This pattern appears vague in other specimens including those of type locality.

Antennae ( Figures 19­22 ). Antennal ratios: in female, ant. I/II/III/IV = 1/1.6/2.3/4.4 and antennal segment/cephalic diagonal = 1.7; in male, ant. I/II/III/IV = 1/1.5/2.8/6 and antennal segment/cephalic diagonal = 1.3. Male antennal segment II with acanthoids setae in three whorls: 2+2+3 and two more on antennal segment I. Additional posteroventral setae of antennal segment IV (ventral sensilla for some authors) on d3, d4 and d5 of male and female ( Figure 20 , arrows); sensorial setae of male on d1 (0­1), d2­3 (1­2), d4, d5 and d6 (2); female: d2­d6 (2) ( Figures 30­31 ). There are 11­12 whorls of structural setae. The antennal segments have a variable level of ringing in different specimens, higher in the male. Sensory organ of antennal segment III with three accessory sensillae, two long and one short.

Head. Eyes: 8+8; eyepatch with two setae. Cephalic setae: mesochaetae, with setae closer to the eye and antennal insertion slowly thickened. Labral formula: 6/5­5­4. Ventral head­back with 1+1 oval organs ( Figure 33 ).

Great abdomen. Trichobothria A, B, and C in a linear pattern. Dorsal setae not different between sexes, and last row longer ( Figures 26 a-b). The number and position of the setae of the great and small abdomen segments of male and female are schematised in Figures 27 and 28. There are two ‘i’ setae before the sensilla ‘s’ on lateral of the great abdomen ( Figures 27-28 ). The group of setae on posterior dorsal great abdomen (rows 9 to 15) includes 28 setae in the female and 24 in the male. There are three long and lightly swollen setae in the first row behind the sensilla and below the small abdomen ( Figures 26 e and 26f). Behind this row there are four more rows with 3, 3-4, 2-3 and 2 setae, respectively.

Legs. Tibiotarsus with oval organ present on the three legs ( Figures 23-24 ). External setae on tibiotarsus III 1.5x longer than diameter ( Figure 24 ). Pretarsus characteristic of the genus, with seta Ja winged ( Figures 15 , 25 ). Spatulated setae formula of whorl I: 3-3- 2 in legs I, II and III, respectively. Claws with large inner tooth (apomorphic character) and other proximal anterior and lateral; empodia without terminal filament ( Figure 18 ).

Ventral tube. Apical flaps with one pair of setae and corpus without seta.

Retinaculum. Tridentate (2+1) and with three apical setae on the corpus.

Furcula. Chaetotaxy as in Figure 32 . Length of the mucro: 50­65 m ( Figure 17 ).

Small abdomen. The males lack the a2 seta on the first row of the small abdomen. There is a small sensilla just behind trichobothria E. Male genital papilla similar in size to ventral anal valves. Two oval organs on each side (hollow arrows in Figures 27­28 ). Length of the anal appendages: 48 m. ( Figures 16 and 35 ).

Taxonomic discussion. The specific diagnostic characteristics of D. bisetosus sp. nov. are: two ‘i’ setae between ‘s’ sensilla on lateral of the great abdomen and the fourth setae of row posterior to trichobothria C ( Figures 27­28 ); the characteristic colouring observed in specimens from localities of the Iberian Peninsula (Navarra and Málaga), even from localities nearly 2000 m high in the Pyrenees (Astún). Although D. bisetosus sp. nov. shares with Navarrella apicalis Bretfeld and Arbea, 2000 the presence of two ‘i’ setae, have the remain characters of the genus Deuterosminthurus . We have seen a cleared specimen, identified as Bourletiella pallipes (Bourlet, 1843) (Gisin collection, MHNG), possibly a D. bisetosus sp. nov. (dark facies) from the northern Pyrenees. A similar range of colouring is observed in D. pallipes , with D. repanda as the pale facies with red patches. Any specimen of D. bisetosus sp. nov. with general dark blue pattern, typical for D. pallipes , has not been found within the captured populations. The dark form of D. pallipes has only an ‘i’ seta (Bretfeld, personal communication).

N. apicalis can be distinguished from D. bisetosus sp. nov. because seta IIi is present only on leg III, the special ventral setae (sensillae for some authors) of the antennal segment IV are on d2, d4 and d5 segments, and four setae are present on retinaculum ( Bretfeld & Arbea, 2000).

In the collection of the Museum of Zoology of the University of Navarra there is a specimen with asymmetry in the special ventral setae of the antennal IV (d2, d4, d5/d3, d4, d5) and with the terminal setae of the small abdomen of the male very similar to those of D. sulphureus.

Specimens of other species have been compared with the new species: B. pallipes (Stach’s collection, PAS), B. repanda Âgren, 1903 (Stach’s collection, Szeptycki leg. and Weiner leg. specimens from PAS and six specimens from MHNG), B. mixta Gisin, 1957 and B. flava (holotype male and paratypes females from Vessi, Gisin collection, MHNG). All of these species have only an ‘i’ seta and a pattern of colouring different to those of the new species.

Figures

FIGURES 14 ­ 18. Deuterosminthurus bisetosus sp. nov. 14. Habitus of a female (Bar: 100 m). 15. Ja seta of leg I (Bar: 2 m). 16. Anal appendage of female (Bar: 10 m). 17. Mucro and detail of its tip (Bar: 10 m). 18. Distal part of leg III (Bar: 10 m)

FIGURES 1 ­ 2. 1. Deuterosminthurus bisetosus sp. nov. with Polydiscia deuterosminthurus sp. nov. on it. 2. Habitus of Polydiscia deuterosminthurus sp. nov. (Bar: 0. 1 mm)

FIGURES 36 - 38. Deuterosminthurus bisetosus FIGURES 39 - 42. Deuterosminthurus bisetosus sp. sp. nov. 36. Colour pattern of a completely pale nov. 39. Colour pattern of a pale specimen from Otazu specimen from Otazu (central Navarra). 37. (north of Navarra). 40. Red coloured specimen from Generalised coloration of the species, with pale ‘ Vedado de Egüaras’ (south of Navarra). 41. Dark patbrown-violet patches. 38. Orange-red coloured tern of a specimen from Benalmádena (Málaga, south specimen from ‘ Vedado de Egüaras’ (southern of Iberian Peninsula). 42. Lateral view of a specimen Navarra). In all cases the intensity of the colou- from Otazu showing the dark insertion area of the triration is variable (Bar: 0,25 mm). chobothria.

FIGURES 19 ­ 25. Deuterosminthurus bisetosus sp. nov. 19. Antenna of male. 20. Antenna of female. 21. Distal part of Ant. IV of a female (identical in the male). 22. Outline of the antenna of other female. 23. Arrangement of the setae on tibiotarsus (according to Nayrolles’s 1987 nomenclature): a, leg I; b, leg II; c, leg III. 24. Setae of tibiotarsus: a, leg I; b, leg II; c, leg III. The hollow arrows points to the oval organs. 25. Whorl I of leg I. (Bar: 0,1 mm, except for C, D and G)

FIGURES 30 ­ 35. Deuterosminthurus bisetosus sp. nov. 30. Antennae of male. 31. Antennae of female (a and b with sensillae of dorsal side; c with special setae of posteroventral side). 32. Chaetotaxy of the furcula (according to Nayrolles’s 1990 nomenclature) (Bar: 0. 1 mm). 33. Chaetotaxy of the head. The white arrow points to the oval organ (abbreviations: c, cervical; v, vertical; o, ocular; d, dorsal; a, anterior; sd, sub­dorsal; g, genal; cp, clipeal posterior; cc, clipeal central; cm, clipeal medial; cma, clipeal medial anterior; ca, clipeal anterior; pl, pre­labral). 34. Seta Sd’ 4 (black arrow in D) (Bar: 0. 0 1 mm). 35. Female anal appendage (Bar: 10 m)

FIGURES 26 ­ 29. Deuterosminthurus bisetosus sp. nov. 26. Some setae of the body. a: row 15 of great abdomen of male; b: row 15 of great abdomen of female; c: row 10 ­ 11 of great abdomen of male; d: row 10 of great abdomen of female; e: seta ventrolateral lightly swollen, posterior to lateral sensilla (arrow in Figure 27), male; f: seta ventrolateral lightly swollen, posterior to lateral sensilla of female; g: second ‘ i’ seta (contiguous to lateral sensilla ‘ s’) of male; h: lateral sensilla ‘ s’ of male; i: second ‘ i’ seta (contiguous to lateral sensilla ‘ s’) of female; j: lateral sensilla ‘ s’ of female; k: trichobothria D of female; l: trichobothria E of female (Bar: 10 m). 27. Chaetotaxy of great and small abdomen of male (nomenclature modified from Betsch & Bretfeld, 1991). The hollow arrows points to the oval organs. 28. Chaetotaxy of great and small abdomen of female. The black arrow points to the setae drawn in 26 f. The hollow arrows points to the oval organs. 29. Lateral colour pattern of a semi­pale specimen from Otazu (Navarra) (Bar: 10 m)