Ituglanis crispim, Donin & Pinna & Severi & Ramos, 2023

Donin, Laura M., Pinna, Mário C. C. de, Severi, William & Ramos, Telton P. A., 2023, Filling in a biogeographic gap: the first Trichomycteridae from the Parnaíba River basin (Siluriformes: Trichomycteridae), Neotropical Ichthyology (e 230018) 21 (2) : -

Ituglanis crispim , new species

urn:lsid:zoobank.org:act:0006F49A-18F7-47AF-A8EB-03D2CBC87268

( Fig. 1; Tab. 1)

Ituglanis sp. —Ramos et al., 2014:04 [listed to Parnaíba River basin].

Holotype. MZUSP 126762 , 28.8 mm SL, Brazil, Piauí, Uruçuí, riacho da Volta, right tributary to Parnaíba River, Parnaíba River basin, 07º24’18”S 44º50’21”W, 18 Jul 2005, W. Severi, B. Dourado & A. Antonello.

Paratypes. All collected with holotype. MZUSP 126756 , 3 (1 c&s), 29.4–32.6 mm

SL; UFPB 12208, 9 (2 c&s), 26.0– 31.3 mm SL.

Diagnosis. Ituglanis crispim is distinguished from all congeners by the combination of the following characters: two or three pairs of ribs; 36–38 post Weberian vertebrae; a color pattern composed of round, uniformly-sized, evenly-spaced, non-coalescent spots; I,5 pectoral-fin rays; the first haemal arch on the 5 th or 6 th vertebra; the first completely fused (to the tip) haemal spine on 15° vertebrae. The presence of two or three pairs of ribs (vs. four pairs or more) distinguish the species from all congeners with the exception of I. amazonicus (Steindachner, 1882) , I. apteryx Datovo, 2014 , I. compactus Castro & Wosiacki, 2017 , I. eichhorniarum (Miranda Ribeiro, 1912) , I. gracilior (Eigenmann, 1912) , I. herberti (Miranda Ribeiro, 1940) , I. ina Wosiacki, Dutra & Mendonça, 2012 , I. inusitatus Ferrer & Donin, 2017 , I. macunaima Datovo & Landim, 2005 , I. metae (Eigenmann, 1917) , I. nebulosus de Pinna & Keith, 2003 , and I. parkoi (Miranda Ribeiro, 1944) . The count of 36–38 post Weberian vertebrae distinguish I. crispim from I. amazonicus , I. apteryx , I. gracilior , I. herberti , I. inusitatus , I. metae , and I. parkoi , all with 39 or more vertebrae. The pigmentation pattern composed by round, similar-sized, non-coalescent dark spots, slightly larger than eye (vs. color patterns either uniform, or mottled with irregular-sized spots or partly coalescent markings, or blotched, sometimes forming longitudinal strips) separates I. crispim from congeners, except for I. amazonicus , I. gracilior , I. guayaberensis , I. macunaima , and I. metae . The presence of I,5 pectoral-fin rays distinguishes I. crispim from I. agreste Lima, Neves & Campos-Paiva, 2013 , I. apteryx , I. bambui Bichuette & Trajano, 2004 , I. boticario Rizzato & Bichuette, 2015 , I. cahyensis Sarmento-Soares, Martins-Pinheiro, Aranda & Chamon, 2006 , I. goya , I. epikarsticus Bichuette & Trajano, 2004 , I. guayaberensis (Dahl, 1960) , I. herberti , I. inusitatus , I. laticeps (Kner, 1863) , I. gracilior , I. macunaima , I. mambai Bichuette & Trajano, 2008 , I. paraguassuensis Campos-Paiva & Costa, 2007, I. parahybae (Eigenmann, 1918) , I. parkoi , I. passensis Fernández & Bichuette, 2002 , I. payaya (Sarmento-Soares, Zanata & Martins-Pinheiro 2011) , I. proops (Miranda Ribeiro, 1908) , and I. ramiroi Bichuette & Trajano, 2004 , all with I,4; I,6 or more. The first haemal arch on 5 th or 6 th vertebrae distinguishes I. crispim from I. bambui (10 th,12 th), I. boticario (8 th), I. compactus (4 th), I. epikarsticus (7 th, 9 th), I. goya (9–10 th), I. nebulosus (4 th), and I. ramiroi (7 th, 8 th, 10 th); the first complete haemal spine on 15 th vertebra distinguishes I. crispim from I. amazonicus (17 th), I. australis Datovo & de Pinna, 2014 (12–13 th), I. eichhorniarum (16 th, 18 th), I. epikarsticus (13–14 th), I. goya , (16 th, 18 th), I. gracilior (16 th, 17 th, 20 th), I. nebulosus (16 th), I. payaya (14 th), I. paraguassuensis (12 th), I. passensis (14 th), I. proops (14 th), and I. ramiroi (12–14 th), (the latter two characteristics have been verified in limited samples of some species, intraspecific variation may be larger than recorded). A putatively autapomorphic character for the new species is the partial or total fusion between the anterior arms of the basipterygium (united minimally for 50% of their length) ( Fig. 2).

Description. External morphology. Morphometric data in Tab. 1. Body elongate, roughly cylindrical in cross-section at trunk, gradually compressed towards caudal peduncle. Dorsal and ventral profiles of trunk slightly convex and slightly concave at caudal peduncle

Head depressed, trapezoid in dorsal view, slightly longer than wide. Dorsal profile straight and ventral profile straight to slightly convex. Snout round in dorsal view. Eyes on anterior half of head, close to posterior nostril. Orbital rim not free. Elliptical ocular capsule formed by thin and transparent skin. Nostrils smaller than eye diameter. Anterior nostril surrounded by fleshy flap of integument posterolaterally continuous with nasal barbel. Posterior nostril surrounded anterolaterally by thin flap of integument. Gill openings narrowly united to isthmus anteriorly, forming free fold. Mouth subterminal and slightly convex in ventral view. Lower lip with lateral fleshy folds continuous with rictal barbel base. Lips covered with small papillae.

Barbels with wide bases, tapering gradually towards tips. Nasal barbel emerging from posterolateral region of anterior nostril, its tip reaching to posterior portion of opercular odontodophore. Maxillary barbel reaching to pectoral-fin base. Rictal barbel reaching to posterior portion of interopercular odontodophore.

Pectoral fin with distal margin convex, first ray unbranched and prolonged as short filament, followed with five branched rays (13*). Pelvic-fin origin anterior to vertical through origin of dorsal fin, with distal margin convex, covering urogenital papilla and almost reaching origin of anal fin. Pelvic fin with one (13*) unbranched ray and four branched rays (13*; 1 specimen with three on one side). Bases of contralateral pelvic fins contacting each other medially (one specimen with bases tightly adpressed to each other).

Dorsal fin with distal margin convex, with two (2 xr*, 1xr–c&s) or three (1xr, 2c&s) procurrent rays, two (11*) or three (2) unbranched rays and six (6) or seven (7*) branched rays. Dorsal fin located on posterior one-third of trunk with fin origin approximately at vertical through posterior margin of adpressed pelvic fin.

Anal fin elongated with distal margin convex, approximately same size of dorsal fin, with two (3 xr*, 1 xr–c&s, 2 c&s) procurrent rays, two unbranched rays (13*) and five branched rays (13*). Origin of anal fin located slightly posterior to vertical through dorsal-fin origin. Caudal fin with distal margin round. Upper plate with one (12*) or two (1) unbranched ray and five (12*) or four (1) branched rays; lower caudal plate with one (10) or two (3*) unbranched rays and six (10) or five (3*) branched rays. Procurrent caudal-fin rays 15 (first of them vestigial) (2 xr*, 1 xr–c&s, 1 c&s) or 14 (1xr) dorsally and ten (1xr, 1xr–c&s,1 c&s), 11 (1xr) or 12 (1xr*, 2 c&s) ventrally.

Osteology. Mesethmoid shaft expanded laterally along anterior half, with convex margins, narrowing at base of cornua. Mesethmoid cornua directed straight laterally, with tips slightly curved posteriorly, gradually narrower toward tip, reaching laterally two-thirds of premaxilla ( Fig. 3A). Anterior cranial fontanel small, teardrop-shaped, its posterior margin slightly posterior to transverse line through exit for infraorbital branch of latero-sensory canal ( Fig. 3A). One specimen with vestigial anterior fontanel, reduced to slight spacing along median frontal suture at corresponding position. Posterior cranial fontanel teardrop-shaped, smaller than anterior one, situated posteriorly in supraoccipital, its center approximately at tranverse line through midlength of pterotic ( Fig. 3A).

Lacrimal-antorbital short, longer than broad, positioned dorsal to anterior portion of autopalatine ( Fig. 4). Barbular long, inserted at lateral-ethmoid-orbitosphenoid limit, gently curved laterally anteriorly; Widest portion of barbular at its anterior third, forming attenuated processes dorsally and ventrally ( Figs. 3–4). Sphenotic with anterior margin protruding anteriorly, clearly diverging from lateral margin of frontal ( Fig. 3A). Sphenotic, prootic, and pterosphenoid fused ( Fig. 3). Trigeminofascialis foramen oval, formed at limit between anterior margin of former bone and posterior margin of orbitosphenoid. Proximal portion of emerged trigeminofascialis nerve following protruded portion of sphenotic dorsal to it. Vomer arrow-shaped, its anterior narrow tip not reaching base of mesethmoid cornua. Lateral arms of vomer large and well-defined, directed posterolaterally ( Figs. 3, 4B). Posterior shaft of vomer long, spikelike, overlapping anterior portion of parasphenoid, nearly to transverse line through midlength of orbitosphenoids. Parasphenoid extending posteriorly as long narrow process reaching anterior third of basioccipital ( Fig. 3B). Weberian capsule small and oval, with anterior margin fused to basioccipital, capsule produced laterally into long tube with small distal openings ( Fig. 3B).

Premaxilla long, with lateral portion narrowing to pointed process flush with anterior margin, with 16–20 conical similar-sized teeth, distributed in two regular rows ( Figs. 3–4). Dentary with 15–22 conical teeth, variable in size distributed in two regular rows not reaching coronoid process. Autopalatine with sinusoid anterior margin, capped with large cartilage articulating anteriorly with premaxilla and maxilla ( Figs. 3–4). Medial margin of autopalatine produced mesially at right angle into broad articular region (= with deep concavity in previous works). Articular surface of palatine bipartite, with

anterior portion articulating with vomer and posterior one with lateral ethmoid. Posterior process of autopalatine overlapping anterior portion of metapterygoid. Metapterygoid longer than broad, tapering posteriorly and ventrally curved, connected to quadrate through narrow cartilage-mediated articulation located at its anterior region, forming roundish fenestra with corresponding recesses in anterodorsal portion of metapterygoid and posterodorsal region of dorsal process of quadrate ( Fig. 5). Quadrate with large anterodorsal process, expanded anteriorly and posteriorly, former expansion triangular

( Fig. 5). Hyomandibula strongly expanded anterodorsally, with well-developed notch (sometimes two, closely positioned) at midlength on dorsal margin ( Fig. 5). Opercular odontodophore oval or round, with 13–19 conical odontodes in circular arrangement (counted in three c&s specimens). Opercle with double articulation, dorsal one with hyomandibula and ventral, smaller one, with preopercle. Opercle with large pointed ascending process. Interopercular odontodophore elongate with 14–19 conical odontodes (counted in three c&s specimens), arranged in two rows and not extending anteriorly beyond articulation with the suspensorium. Opercular and interopercular odontodes increasing in size posteriorly ( Fig. 5).

Ventral (and only) hypohyal triangular. Anterior ceratohyal elongate, expanded at both anterior and posterior ends, with plane of expansions orthogonal to each other. Posterior ceratohyal short and roughly triangular, narrowing markedly from base to tip. Eight branchiostegal rays: three in contact with anterior ceratohyal, four closely-set on interceratohyal cartilage, and posterior one distantly connected with rest of series. Four posterior branchiostegal rays expanding distally ( Fig. 6). Interhyal absent. Parurohyal with well-defined anterior arms diverging anterolaterally and wide posterolateral wings narrowing distally, with round tips. Parurohyal with fine posterior process and wide round hyoid foramen ( Fig. 7).

Basibranchials 2 and 3 elongate, connected to each other by cartilage; basibranchial 2 with gentle expansions at anterior and posterior ends, longer than rod-like basibranchial 3. Basibranchial 4 catilaginous, pentagonal with concave edges. Hypobranchial 1 rod-like, slightly broader laterally than mesially, with cartilaginous tips. Hypobranchial 2 mostly cartilaginous, oriented obliquely to anteroposterior axis, with small conical ossification at anterolateral corner. Hypobranchial 3 mostly cartilaginous, twice as broad as hypobranchial 2 and with anterolateral bony cap similar in shape to that of hypobranchial 2 but twice as large. Five elongate ceratobranchials with cartilaginous tips. Ceratobranchials 1 to 4 abruptly and variably narrowing towards proximal ends. Ceratobranchial 5 shorter than preceding elements, with 10–12 conical teeth irregularly concentrated on dorsal surface near mesial margin of anterior half of bone. Gill rakers present on ceratobranchials 3–5, weakly ossified, spaced out and loosely attached. Vestigial ossified raker elements occasionally also present in ceratobranchial 2. Epibranchial 1 with large pointed uncinate process at midlength of anterior margin; epibranchial 2 with small process in anterior margin; epibranchial 3 with rounded process on posterior margin. Epibranchial 4 rectangular, with wide articular surface with upper pharyngeal plate and narrow one with ceratobranchial 4. Pharyngobranchials 1 and 2 absent. Pharyngobranchial 3 small, rod-like, similar in shape to hypobranchial 1, but smaller in size. Pharyngobranchial 4 cartilaginous and attached to dorsolateral surface of very large, curved, upper pharyngeal plate, the latter with conical teeth, arranged in two irregular rows, increasing in size posteriorly ( Fig. 8).

Pelvic bone small in overall size, with small basal plate produced mesially into flared articular surface for its counterpart. Cartilages for fin rays and interbasipterygial articulation thick and well developed, with two cartilaginous regions continuous or nearly so. Anterior arms of basipterygium variable. One specimen with one side deformed, the other with two separate arms united by medial lamina of bone for at least 50% of their length. Another specimen with one side partly separate as in previous specimen and the other side entirely fused, forming single basally broad anterior arm, tapering to fine tip ( Fig. 2). Third specimen with one side with arms partly separate as in previous specimens and the other side with arms almost entirely fused but retaining separate tips, fused region broad for entire length, not tapering. Fused morphology of basipterygial arms apparently extreme condition of partial fusion observed in specimens with separate arms.

Dorsal fin with eight (2 xr, 1xr–c&s, 2c&s) or (1xr*) nine pterygiophores, first one inserted anterior to neural spine of 24 th (1xr), 23 rd (2 xr*, 1 xr–c&s) or 22 nd (1c&s) vertebrae. Anal fin with six pterygiophores (3 xr*, 1xr–c&s, 2c&s), first one inserted anterior to haemal spine of 25 th (xr), 24 th (2xr*, 1xr–c&s) or 23 rd (1c&s) vertebrae. Epural variable, present as a comma-shaped bone in two specimens (1xr, 1xr–c&s), as a small round distal element in two (1xr, 1c&s) and absent in two (1 xr*, 1c&s). Uroneural independent of hypural elements. Upper hypural plate as single element (presumably including fused hypurals 3, 4 and 5) or with two elements, a slightly smaller upper one (presumably independent hypural 5) and a slightly larger lower one (presumably fused hypurals 3 and 4) (relative sizes of each element not matching usual proportions of such components in other trichomycterid taxa) ( Figs. 9–10). Lower hypural plate (presumably co-ossified parhypural and hypurals 1 and 2) fused to compound caudal centrum ( Figs. 9–10). Branched caudal fin splitting once. Post-Weberian vertebrae 36 (1c&s), 37 (1 1xr–c&s) or 38 (3 xr*); ribs two (1 xr, 1c&s) or three (2 xr*, 1xr–c&s) ( Fig. 3); first haemal arch on vertebrae 5 th (2 c&s) or 6 th (1 c&s); first fully fused haemal spine on vertebrae 15 th (3 c&s).

Laterosensory system. Laterosensory canals with simple (non-dendritic) branches ending in single pores. Nasal and frontal canals of supraorbital branch continuous, with three pores (s1, s3, and s6). Supraorbital pores s1 and s3 located at posterior portion of anterior and posterior nostrils, respectively; supraorbital pore s6 aligned with posterior margin of eyes.

Antorbital segment of infraorbital canal absent. Sphenotic canal present with two pores, i10 and i11 (one specimen with three pores on right side). Otic, postotic, and scapular canals present, with preoperculomandibular branch and pterotic branch short and with one associated pore each. Postotic canal with preoperculomandibular and pterotic branches located anterodorsal to opercular odontodophore; po1 pore located at horizontal line through i11; pterotic pore po2 (located at posterior margin of opercular odontodophore, medial to preoperculo-mandibular pore. Trunk canal short with two pores (one specimen with three pores on left side; one with three pores on right side and one with one pore on left side) anteriorly located between pectoral-fin base and opercular odontodophore.

Coloration in alcohol. Dorsal and lateral surface of body and head with uniform covering of round, similar-sized, non-coalescent dark spots, most of which slightly larger than eye ( Fig. 1). Spots uniformly spaced, entirely individualized, separated from each other by thin frames of white. Markings not forming rows, stripes or concentrations anywhere on body, including middorsal and midlateral areas. Dark covering fading abruptly below limit of abdominal cavity along abdomen, but extending to ventral limit of body posterior to posterior margin of pelvic fins. Abdomen white, with some specimens (including holotype) with few faint spots anterior to pelvic-fin insertion. Dorsal and lateral surface of head with covering of spots similar to those on body, but slightly smaller in size. Ventral side of lower lip with few dark spots. Opercular odontodophore white in central portion but with dark streaks on surrounding periodontodal fold. Interopercular odontodophore darkly pigmented in central area, and predominantly white around margin in most specimens, in some others dark coloration irregular and not segregated between peripheral and central areas. Maxillary barbel with irregular dark markings on dorsal surface, most concentrated on proximal half. Nasal barbel pigmentation similar to that of maxillary one, but on both surfaces. Rictal barbel white, except for few dark spots on dorsal surface near base. Pectoral fin with elongate dark fields over rays until about two-thirds of their lengths, darkest along edge of first ray. Dorsal fin with concentration of spots along base, fading in middle of fin and then concentrated again at distal third, not reaching edge of fin. Anal-fin with dark covering similar to that of dorsal one, but fainter. Caudal fin with covering of elongate dark fields along entire length, forming faint jagged vertical rows. Distal margin of caudal fin white. Pelvic fin white.

Geographical distribution. Itugalnis crispim is known so far from a single site in the riacho da Volta, right tributary to Parnaíba River, Parnaíba River basin ( Fig. 11).

Ecological notes. Ituglanis crispim is so far known from a small clear water perennial creek, with moderate current, hard clay bottom, submerged filamentous algae, and riparian vegetation typical of the Cerrado biome, at ca. 200 m a.s.l. ( Fig. 12). No other fish species were found at that spot. Stomach contents of two c&s specimens show larvae of Diptera ( Simuliidae ), Nematoda, and unidentified remains of insects. No evidence was found of mature gonads in external examination. Dissection of alcoholic specimens was not attempted because of rarity of specimens.

Etymology. The specific epithet was given in reference to the Crispim, known for the local tragedy-legend “Cabeça de Cuia”. Having murdered his own mother, Crispin was cursed and turned into a horrific aquatic creature with a gigantic “cuia” (bowlshaped) head, condemned to perpetually wander the Parnaíba River and only to be redeemed after devouring seven virgins named Maria. A noun in apposittion.

Conservation status. Ituglanis crispim is endemic to Parnaíba River basin and known so far from a single locality in a small tributary within the Parnaíba River drainage. The lack of any additional records of the species, either in the type locality and elsewhere since 2005, despite at least one additional sampling attempt, suggests that the species is narrowly endemic and perhaps locally rare also. Despite the extremely restricted geographic range, no specific threats have been detected, therefore I. crispim can be categorized as Least Concern (LC) according to IUCN categories and criteria (IUCN Standards and Petitions Subcommittee, 2022). However, the apparently narrow distribution, lack of environmental monitoring of the type locality and the absence of any additional specimens since the type series, indicate that survival of the species is highly dependent on the preservation of its watercourse and of the Cerrado biome around its known range.