Leptographium francke-grosmanniae (R.W. Davidson) K. Jacobs & M.J. Wingf., Leptographium species: p. 99 (2001)

Yin, Mingliang, Wingfield, Michael J., Zhou, Xudong, Linnakoski, Riikka & Beer, Z. Wilhelm de, 2019, Taxonomy and phylogeny of the Leptographium olivaceum complex (Ophiostomatales, Ascomycota), including descriptions of six new species from China and Europe, MycoKeys 60, pp. 93-123 : 93

Leptographium francke-grosmanniae (R.W. Davidson) K. Jacobs & M.J. Wingf., Leptographium species: p. 99 (2001)

≡ Ceratocystis francke-grosmanniae R.W. Davidson, Mycologia 63: 6 (1971). (Basionym)

≡ Ophiostoma francke-grosmanniae (R.W. Davidson) De Hoog & R.J. Scheff., Mycologia 76: 297 (1984).

≡ Grosmannia francke-grosmanniae (R.W. Davidson) Zipfel, Z.W. de Beer & M.J. Wingf., Stud. Mycol. 55: 90 (2006).

Type.

GERMANY, Reinbeck near Hamburg, from Quercus sp. associated with Hylecoetus dermestoides , May 1967, H. Francke-Grosmann, (holotype BPI 595654, ex-holotype cultures: RWD 828 = ATCC 22061 = CBS 356.77 = CMW 445).

Descriptions.

Davidson (1971, pp 6-7, figs 1, 10, 11, 17); Upadhyay (1981, p. 45, figs 73-78); Mouton et al. (1992, figs 1-11); Wingfield (1993, p. 48, figs 6-7); Jacobs and Wingfield (2001, pp 99-102, figs 73-75).

Host tree.

Quercus sp.

Insect vector.

Hylecoetus dermestoides .

Distribution.

Germany.

Notes.

Leptographium francke-grosmanniae groups peripheral to other species in the L. olivaceum complex ( Figs 1 - 3 ). Morphologically, the ascospores are almost cylindrical and its ascomatal necks correspond with other species in the complex. But L. francke-grosmanniae produces mononematous conidiophores, in contrast to the synnemata produced by the other species, which also explains why it is the only species in the complex previously treated in Leptographium . The mode of conidiogenesis of L. francke-grosmanniae ( Mouton et al. 1992) appears similar to that of other species where the conidiogenous cells that appear phialidic under a light microscope arise from percurrent proliferation ( Wingfield et al. 1989, Wingfield et al. 1991, Mouton et al. 1993). However, the apices of the apparent “phialides” are substantially more flared than those of other species in the complex and they could be more different than assumed by Mouton et al. (1993). Leptographium francke-grosmanniae is also unusual in the L. olivaceum complex in having an angiosperm host.

Leptographium francke-grosmanniae was originally described as Ceratocystis francke-grosmanniae from larval galleries of Hylecoetus dermestoides on Quercus sp. in Germany ( Davidson 1971). De Beer and Wingfield (2013) showed that sequences for this species produced in different studies were inconsistent. Based on comparisons of the ITS2 region, the sequences of ex-holotype generated in the present study are consistent with those produced by Mullineux and Hausner (2009) for ATCC 22061 and Hamelin et al. (unpublished) for CBS 356.77, but differ substantially from sequences produced by Jacobs et al. (2001b). In the LSU gene region, our sequences are identical to those of Hausner et al. (2000), but they differed from that of Jacobs et al. (2001a, b) for CMW 445.In the β-tubulin gene region, the sequence of CMW 445 in the present study was consistent with that provided by Kim et al. (2004) for CMW 445 and Hamelin et al. (unpublished sequence in GenBank) for CBS 356.77. We thus suggest that the two sequences for L. francke-grosmanniae produced by Jacobs et al. (2001a, b) are incorrect. Sequences of another isolate from the USA (CMW 2975), previously identified as L. francke-grosmanniae ( Zipfel et al. 2006), differ substantially from the ex-holotype culture. Thus, this isolate (CMW 2975) does not represent L. francke-grosmanniae , and its taxonomic placement needs reconsideration.

Figures

Figure 1. Left side: ML tree of the genus Leptographium generated from the ITS 2 - LSU DNA sequence data. Sequences generated from this study are printed in bold type. Bold branches indicate posterior probabilities values ≥ 0.95. Bootstrap values ≥ 75 % are recorded at nodes as ML / MP. * Bootstrap values <75 %. Scale bar represents 5 nucleotide substitutions per 100 nucleotides. Right side: ML trees of the L. olivaceum complex generated from the DNA sequences of combined four protein-coding gene regions, including ACT, CAL, TEF- 1 α, and TUB. Bold branches indicate posterior probabilities values ≥ 0.95. Bootstrap values ≥ 75 % are recorded at nodes as ML / MP. * Bootstrap values <75 %. Scale bar represents 5 nucleotide substitutions per 100 nucleotides.

Figure 3. Leptographium breviuscapum sp. nov. (CMW 38888) a fourteen-days old culture on OA with black background b synnematous asexual state on wood tissue on WA c-d conidiophore e conidiogenous cells f conidia. Scale bars: 100 μm (b), 25 μm (c), 25 μm (d), 10 μm (e), 5 μm (f).