Roussoella pseudohysterioides D.Q. Dai & K.D. Hyde

Wijayawardene, Nalin N., Dissanayake, Lakmali S., Li, Qi-Rui, Dai, Dong-Qi, Xiao, Yuanpin, Wen, Ting-Chi, Karunarathna, Samantha C., Wu, Hai-Xia, Zhang, Huang, Tibpromma, Saowaluck, Kang, Ji-Chuan, Wang, Yong, Shen, Xiang- Chun, Tang, Li-Zhou, Deng, Chun-Ying, Liu, Yanxia & Kang, Yingqian, 2021, Yunnan-Guizhou Plateau: a mycological hotspot, Phytotaxa 523 (1), pp. 1-31 : 9

Roussoella pseudohysterioides D.Q. Dai & K.D. Hyde ,

Fungal Diversity 82: 37 (2016)

( FIGURE 5 )

Index Fungorum Number: IF552026

Saprobic on decaying bamboo culms. Sexual morph: Ascostromata forming under black area, including 3–5 locules, up to 3–5 mm long and 0.5–2 mm wide, slightly raised at maturity, irregular, black, coriaceous. Locules in vertical section 220–280 μm high, 180–330 μm diam., gregarious, subglobose to ellipsoidal, dark brown, with ostiolate opening. Peridium composed of dark brown cells comprising host and fungal tissues. Hamathecium comprising dense, 2–3.5 μm wide, cellular pseudoparaphyses, indistinctly septate, embedded in a gelatinous matrix. Asci 85–290 × 7.5–17.5 μm (x = 165×10.5 μm, n=30), 8-spored, bitunicate, cylindrical, with a short furcate pedicel, with an apical ocular chamber. Ascospore s 11–19.5 × 4–6.5 μm (x = 16.5×5.5 μm, n=30), uniseriate, fusiform-ellipsoidal, 1-septate, constricted at the septum, narrow at both ends, with striate wall ornamentation, some with obvious verrucose. Asexual morph: Undetermined.

Material examined:— CHINA, Guizhou Province, Leigong Mountain National Nature Reserve , on dead culm of bamboo, July 2019, Q. R. Li 2019LGS13 ( GMB0009 ), living cultures, GMBC0009 (new country record) .

Known hosts and distribution:— Guizhou, China, Thailand

Known hosts:— Bamboo

GenBank Numbers:— ITS: MW881445 ; LSU: MW 881451 ; RPB2: MW 883345

Notes:— Roussoella , typified by Roussoella nitidula Sacc. & Paol. was introduced by Saccardo & Paoletti (1888). Most species of Roussoella were observed from monocotyledon, such as bamboo and palms ( Dai et al. 2017; Hyde et al. 2018). Roussoella pseudohysterioides was originally introduced by Dai et al. (2017) isolated from Thailand. This is the first report of Roussoella pseudohysterioides discovered from China.

Figures

FIGURE. Roussoella pseudohysterioides (GMB0009). a–d. Ascostromata developing on bamboo culm. e, f. Vertical sections of ascostromata. g–j. Asci containing eight ascospores. k. Fragment of ascostromata in KOH without stromatal pigments. l–m.Ascus apex in Melzer’s reagent. n–r. Dark brown ascospores. Scale bars: j–r = 10 μm.