Diaporthe guangzhouensis Ning Jiang & Y. Q. Zhu, 2024

Zhu, Yaquan, Ma, Lei, Xue, Han, Li, Yong & Jiang, Ning, 2024, New species of Diaporthe (Diaporthaceae, Diaporthales) from Bauhinia variegata in China, MycoKeys 108, pp. 317-335 : 317-335

Diaporthe guangzhouensis Ning Jiang & Y. Q. Zhu sp. nov.

Fig. 4

Etymology.

Named after the collection site of the type specimen, Guangzhou City.

Holotype.

China • Guangdong Province, Guangzhou City, Longdong straight street , 23 ° 11 ' 41.02 " N, 113 ° 22 ' 8.33 " E, 46 m asl, on diseased leaves of Bauhinia variegata , 8 Aug 2022, Yong Li, Chengbin Wang & Yaquan Zhu, (holotype: CAF 800095 ; ex-type culture: CFCC 58151 ).

Description.

Conidiomata pycnidial, scattered to aggregated, black, erumpent, raising above surface of culture medium, subglobose, 150–450 µm diam, exuding white or yellowish creamy conidial droplets from central ostioles after 30 days at 25 ° C. Conidiophores 12.5–24.5 × 1–2.5 μm, cylindrical, hyaline, unbranched, straight to sinuous. Conidiogenous cells densely aggregated, phiailidic, unbranched, straight or slightly curved, 5.5–10 × 2.0–7.5 μm. Beta conidia filiform, hyaline, straight or slightly curved, aseptate, 17.0–29.5 × 1.0–2.0 μm. Alpha conidia and gamma conidia not observed. Teleomorph not observed.

Culture characteristics.

Colonies covering entire plate after 2 weeks. On PDA with profuse aerial mycelium, white surface, reverse amber. On MEA with fluffy aerial mycelium, dirty white surface, reverse ochreous. On SNA white sparse aerial mycelium, surface and reverse white.

Additional material examined.

China • Guangdong Province, Guangzhou City, Longdong straight street , 23 ° 11 ' 41.02 " N, 113 ° 22 ' 8.33 " E, 46 m asl, on diseased leaves of Bauhinia variegata , 8 Aug 2022, Yong Li, Chengbin Wang & Yaquan Zhu, living culture GZ 13 E .

Notes.

Diaporthe guangzhouensis from the present study is phylogenetically close to D. tulliensis (Fig. 2 ). Diaporthe guangzhouensis differs from D. tulliensis in nucleotide sequence data (5 / 526 in ITS, 9 / 347 in tef 1, 13 / 711 in tub 2) ( Crous et al. 2015). In addition, host and distribution data are vital for species identification ( D. guangzhouensis inhabiting Bauhinia variegata in China vs. D. tulliensis inhabiting Theobroma cacao in Australia) ( Crous et al. 2015).

Figures

Figure 2. Phylogram of Diaporthe sojae species complex resulting from a maximum likelihood analysis based on a combined matrix of ITS, cal, his 3, tef 1 and tub 2 loci. Numbers above the branches indicate ML bootstrap values (left, ML BS ≥ 50 %) and Bayesian posterior probabilities (right, BPP ≥ 0.9). Isolates from the present study are in bold and ex-type strains are marked with *.

Figure 4. Morphology of Diaporthe guangzhouensis A colonies on PDA, MEA and SNA at 25 ° C after 2 weeks B the leaf of Bauhinia variegata C conidiomata D conidiogenous cells with attached beta conidia E – G beta conidia. Scale bars: 200 µm (C); 10 µm (D – J).