Cynodon dactylon, (L.) Pers.1805, (L.) Pers., 1805

Jha, Rajeev Kumar, Wisoyo, Soy Daniel & Srisombat, Sarayut, 2021, Efficacy of Doob grass, Cynodon dactylon against white spot syndrome virus in Penaeus monodon, International Journal of Fisheries and Aquatic Studies 9 (1), pp. 107-112 : 109-110

3.1. Analysis of C. dactylon extract by GCMS

Identification and quantification of individual phenolic compounds before and after heating at 85 0 C of the ethanol extract of C. dactylon was performed by GCMS analysis ( Figure1a and 1b ). The results indicated that few of the ethanolic fraction, acetonitrile fraction, methanol fraction, and hexane fraction of C. dactylon were missing after heating.

A clear pick could be seen missing after heating of the Cynodon extract in GCMS chromatogram. The number of potential compounds marked were about 246 in number, acetonitrile fraction 36, methanol fraction 117 and hexane fraction 103. Any of them or combination of certain compounds have anti-WSSV effect.

Figures

Fig 1a: GCMS chromatogram of Cynodon dactylon extract showing fraction peaks at normal (300C) temperature. The peak in circle shows the original presence of ingredients in the C. dactylon before heating at higher temperature.