Tubeufia guttulata J. Ma & Y.Z. Lu, 2023

Ma, Jian, Zhang, Li-Juan, Boonmee, Saranyaphat, Xiao, Xing-Juan, Liu, Ning-Guo, Xiao, Yuan-Pin, Luo, Zong-Long & Lu, Yong-Zhong, 2023, Morphological and phylogenetic analyses reveal three new species and one new record of Tubeufia (Tubeufiales, Tubeufiaceae) from southern China, MycoKeys 99, pp. 87-108 : 87

Tubeufia guttulata J. Ma & Y.Z. Lu sp. nov.

Fig. 2

Etymology.

The epithet ‘‘ Tubeufia guttulata ’’ refers to the guttulate conidia of this taxon.

Holotype.

HKAS 128936

Description.

Saprobic on decaying wood in a terrestrial habitat. Sexual morph Undetermined. Asexual morph Hyphomycetous, helicosporous. Colonies on natural substrate superficial, effuse, gregarious, white. Mycelium partly immersed, hyaline to pale brown, septate, branched hyphae, smooth, with masses of crowded, glistening conidia. Conidiophores macronematous, mononematous, flexuous, cylindrical, branched or unbranched, septate, 101-247 μm long, 5.5-8 μm wide (x̄ = 165 × 7 μm, n = 20), the lower part pale brown and the upper part hyaline, smooth-walled. Conidiogenous cells holoblastic, mono- to polyblastic, integrated, sympodial, intercalary or terminal, cylindrical, with a denticulate protrusion, truncate at apex after conidial secession, 9-16 μm long, 4-6 μm wide (x̄ = 12 × 5 μm, n = 25), hyaline to pale brown, smooth-walled. Conidia solitary, acropleurogenous, helicoid, rounded at tip, 25-34 μm diam and conidial filament 4-6 μm wide (x̄ = 29 × 5 μm, n = 30), 170-220 μm long (x̄ = 189 μm, n = 30), indistinctly septate, coiled 11/4-21/4 times, becoming uncoiled in water, guttulate, hyaline, smooth-walled.

Culture characteristics.

Conidia germinating on water agar and producing germ tubes within 8 h. Colonies on PDA circular with umbonate surface and undulate edge. Growth rate 21 mm diam in 42 days at 25 °C, with a brown center with pale brown edges on PDA.

Material examined.

China, Hainan Province, Wuzhishan City, Shui Man Town, Wuzhishan National Nature Reserve , 18°92′N, 109°63′E, on rotting wood in a terrestrial habitat, 26 December 2021, Jian Ma, WZS70 (HKAS 128936, holotype; GZAAS 23-0408, isotype), ex-type living cultures CGMCC, GZCC 23-0404; Ibid., WZS99 (GZAAS 23-0593, paratype), living culture GZCC 23-0590 .

Notes.

Tubeufia guttulata is a sister species to T. cocois with 100% ML/1.00 PP supports, however, the phylogenetic tree shows that they are distinct species. Morphologically, Tubeufia guttulata differs from T. cocois in that it has longer conidiophores (101-247 μm vs. 38-123 μm) and larger conidia (170-220 μm vs. 116-136 μm). In addition, the helicoid conidia of T. guttulata become uncoiled in water, while T. cocois are coiled ( Tian et al. 2022).

Figures

Figure 2. Tubeufia guttulata (HKAS 128936, holotype) a, b colonies on the host surface c-e conidiophores, conidiogenous cells and conidia f-i conidiogenous cells bearing conidia j germinated conidium k-p conidia q, r colonies on PDA, q from above r from below. Scale bars: 50 μm (c-e); 20 μm (h-p); 10 μm (f, g).