Talaromyces amyrossmaniae Rajeshkumar, Yilmaz & Seifert

Rajeshkumar, Kunhiraman C., Yilmaz, Neriman, Marathe, Sayali D. & Keith A. Seifert,, 2019, Morphology and multigene phylogeny of Talaromycesamyrossmaniae, a new synnematous species belonging to the section Trachyspermi from India, MycoKeys 45, pp. 41-56 : 46-48

Talaromyces amyrossmaniae Rajeshkumar, Yilmaz & Seifert sp. nov. Figure 3

Etymology.

Latin, named after Dr Amy Y. Rossman, in honour of her career as a research leader in Systematic Mycology and Microbiology, USDA ARS, Beltsville, Maryland, USA.

Diagnosis.

Synnemata abundant in nature, determinate, 90-120 µm tall, with an unbranched stalk 10-35 µm wide, base wider, up to 50-60 µm. Synnema stipe orange red or vivid orange red, capitulum terminal, compact, globose with conidiophores and a powdery grey-green conidial mass in closely packed, split columns. On MEAbl synnemata produced after 2 weeks incubation, up to 120 µm long. Conidiophores biverticillate, with sometime terverticilate sub-branches. Acerose phialides producing smooth to slightly roughened globose to subglobose conidia. Restricted growth on all media, acid production absent on CREA.

In: Talaromyces section Trachyspermi .

Type.

INDIA, Maharashtra, Mahabaleshwar, Lingmala falls; isolated from fallen decaying fruits and litter of Terminalia bellerica ( Combretaceae ), 9 June 2009, isolated by K.C.Rajeshkumar, holotype: AMH 9330, extype: NFCCI 1919, other culture NFCCI 2351.

Gene sequences: ex-holotype MH909062(ITS), MH909064(BenA), MH909068(CaM), MH909066(RPB2).

Description.

Colony diameter, 7 d (mm): CYA 4-6; CYA 37 °C no growth; MEAbl 12-14; YES 5-7; DG18 4-5; OA 10-13; CREA 3-5.

Colony characters: CYA 25 °C, 7 d: colonies low, plane; margins low, entire (<1 mm); mycelia white; no germination; sporulation absent; soluble pigmentation absent; exudates absent; reverse Yellowish white (4A2). MEAbl 25 °C, 7 d: colonies low, slightly raised, synnemata present; margins low, entire (1 mm); mycelia white; texture velvety; sporulation dense (except margins); conidia en masse Dull green (27D4-27E4); soluble pigmentation yellow; exudates orange to reddish orange small droplets; reverse Hazel brown (6E6) at center fading into Light brown (6D8) to Light yellow (3A5). YES 25 °C, 7 d: colonies slightly raised, sulcate, sunken at center; margins low, entire (<1 mm); mycelia pale pinkish red, with and appearance of Pastel red (7A4-7A5); texture floccose; sporulation absent; soluble pigmentation absent; exudates absent; reverse Light brown (7D6). DG18 25 °C, 7 d: colonies slightly raised, sulcate, sunken at center; margins low, entire (1 mm); mycelia pale yellow; texture floccose; sporulation moderately dense at center, margins absent; conidia en masse Greyish green to Dull green (26C4-26D4); soluble pigmentation absent; exudates absent; reverse Brownish orange to Brownish yellow (5C6) in the center, fading into Light yellow (4A5). OA 25 °C, 7 d: colonies low, plane; margins low, entire (<1 mm); mycelia white; texture velvety; sporulation moderately dense at center, margins absent; conidia en masse Greyish green (27C4-27D4); soluble pigmentation dark red; exudates absent; reverse Brown (7E8) in the centre, fading into Copper red (7C8). CREA 25 °C, 7 d: acid production absent.

Micromorphology.

Determinate synnemata formed after 2 weeks on MEAbl up to 80-150 µm long. Conidiophores biverticillate with a minor proportion having subterminal branches; stipes smooth walled 80-120 × 3-4 µm; extra branches up to 30 µm long; metulae three to six, divergent, 10-13 × 2.5-3 µm; phialides acerose, three to six per metulae, 12-15 (-18) × 2-3 µm; conidia smooth, globose to subglobose, 2.5-4 × 2.5-3.5 µm. Sometimes it produces large-sized conidia up to 6-8 µm. Ascomata not observed.