Neodendryphiella tarraconensis Iturrieta-Gonzalez , Gene & Dania Garcia

Iturrieta-Gonzalez, Isabel, Gene, Josepa, Guarro, Josep, Castaneda-Ruiz, Rafael F. & Garcia, Dania, 2018, Neodendryphiella, a novel genus of the Dictyosporiaceae (Pleosporales), MycoKeys 37, pp. 19-38 : 19

Neodendryphiella tarraconensis Iturrieta-Gonzalez, Gene & Dania Garcia sp. nov. Fig. 4

Etymology.

Name refers to Tarragona, the geographical area where the fungus was collected.

Type.

Spain, Tarragona, from garden soil, Feb. 2017, I. Iturrieta-González (holotype CBS H-23479, culture ex-type CBS 144324 = FMR 16234).

Description.

Mycelium superficial and immersed abundant, composed of septate, branched, smooth to verruculose, hyaline to pale brown hyphae, 1-2 μm wide. Conidiophores macronematous, mononematous, erect or slightly flexuous, branched or unbranched, up to 6-septate, cylindrical, 19-185 × 2-5 μm, brown, smooth, darker and finely verrucolose towards the base. Conidiogenous terminal and intercalary, subcylindrical to clavate, 9-35 × (2 –)3–4(– 5) μm, with up to 2 pores. Ramoconidia (0 –)1–2(– 3)-septate, usually slightly constricted at the septa, with up to 3 terminal and subterminal pores, pale brown, smooth to verruculose, mostly cylindrical, with rounded apex and truncate base, 12 –21(– 23) × 4-5 μm. Conidia catenate, with up to 7 conidia in the terminal unbranched part, (0 –)1– 2-septate, pale brown, verruculose, ellipsoidal or subcylindrical with more or less rounded ends, 6-21 × 3 –6(– 7) μm; when 1-septate, the septum is often submedial and slightly constricted, when 2-septate, usually constricted at only one septum.

Culture characteristics

(14 d at 25 °C). Colonies on PDA reaching 23 mm diam., umbonate, velvety, greyish-brown to olive brown (5E3/4F8), with slightly fimbriate margin; reverse dark green (30F8) to black. On PCA attaining 24 mm diam., flat, velvety, olive brown (4F8), slightly fimbriate margin, reverse dark green to olive brown (28F5/3B2) with a pale yellow (4A3) diffusible pigment. On OA reaching 30 mm diam., flat, slightly granular, yellowish-brown to olive brown (5F8/4F4), aerial mycelium scarce, with regular margin; reverse yellowish-brown to olive brown (5F8/4F4). On SNA attaining 21 mm diam., flat, slightly granular, yellowish-brown to olive (5F4/3F5), aerial mycelium scarce, with fimbriate margin; reverse yellowish-brown to olive (5F4/3F5). On MEA reaching 8-10 mm diam., slightly elevated but depressed at the centre, radially folded, velvety, olive (2F8), with irregular margin; reverse olive (2F4).

Cardinal temperature for growth.

Optimum 25 °C, maximum 30 °C, minimum 10 °C.

Distribution.

Spain.

Notes.

In addition to the genetic differences mentioned above, N. tarraconensis differs from the other two species in the genus by the presence of ramoconidia with up to 3 septa and conidia from terminal branches with mostly 1-2-septate. It is noteworthy that 1-septate conidia usually show a slightly longer basal cell since the septum is submedial and, when 2-septate, often only one of the septa is constricted, features not described in any species of Dendryphiella and Neodendryphiella .