Paecilomyces clematidis Spetik, Eichmeier, Gramaje & Berraf-Tebbal, 2022

Spetik, Milan, Berraf-Tebbal, Akila, Gramaje, David, Mahamedi, Alla Eddine, Stusková, Katerina, Burgova, Jana & Eichmeier, Ales, 2022, Paecilomyces clematidis (Eurotiales, Thermoascaceae): a new species from Clematis root, Phytotaxa 559 (3), pp. 238-246 : 242-243

Paecilomyces clematidis Spetik, Eichmeier, Gramaje & Berraf-Tebbal , sp. nov. ( Fig. 2 )

Mycobank number: MB843540

Type: CZECH REPUBLIC, Breclav: Lednice , university garden (48°47’33.3”N, 16°47’55.7”E), isolated from the root of Clematis ‘Snow Queen’ ( Ranunculaceae ), May 2021, M. Spetik, Holotype: BRNU 677844 , ex-type living culture CBS 148466 = MEND-F-0560 .

Sexual morph: not observed. Asexual morph: Hyphomycetous. Conidiophores hyaline, septate, irregularly branched. Phialides with a cylindrical basal portion tapering to a thin long neck, solitary directly on vegetative hyphae or in groups of two–four per metula. Conidia (2.5–)3.3–3.6(–4.2) × (1.7–)2.2–2.5(–3.2) µm, mean ± SD 3.4 ± 0.4 × 2.4 ± 0.4 µm hyaline, ellipsoidal with flattened base, pyriform, produced in chains. Chlamydospores usually produced laterally or terminally on short side branches of superficial or immersed hyphae, thick-walled, initially smooth later becoming rough and warted, subglobose to globose, dark green, 4.5–6.2 µm diam. Culture characteristics. On PDA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous, almost no growth at 18 °C;> 90 mm diam at 25°, 30° and 35 °C; 42 mm diam at 40 °C; no growth at 45 °C. On MEA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 °C. On OA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 °C. On CYA after seven days: white to olivaceous, floccose, aerial mycelium, reverse olivaceous,> 90 mm diam at 25° and 30 °C.

Etymology: —refers to the host genus ( Clematis ).

Additional specimens examined: CZECH REPUBLIC, Breclav: Lednice , university garden (48°47’33.4”N, 16°47’55.7”E), isolated from the root of Clematis ‘ Snow Queen’ ( Ranunculaceae ), May 2021, M. Spetik, living culture MEND-F-0561 .

Figures

FIGURE 2. Paecilomyces clematidis (ex-type CBS 148466). A, F–G. Chlamydospores. C. Conidiophores with phialides. B, D. Phialides forming conidia. E. Conidia. H. Colony on PDA. I. Colony on MEA. J. Colony on OA. Scale bars: 10 μm.