Madagascaromyces cannae Y.X. Zhang, J.W. Chen & Manawas., 2022

Chen, Jingwen, Manawasinghe, Ishara S., Lin, Jieying, Lin, Yanhong, Wanasinghe, Dhanushka N. & Zhang, Yunxia, 2022, Madagascaromyces cannae sp. nov. (Mycosphaerellaceae) on Canna indica (Cannaceae) from Guangdong, China, Phytotaxa 561 (1), pp. 53-64 : 58-60

Madagascaromyces cannae Y.X. Zhang, J.W. Chen & Manawas. sp. nov. ( FIGURE 2 )

Index Fungorµm number: IF 559623

Facesoffungi number: FoF 10814

Etymology: Epithet refers to the host genus from which the fungus was isolated

Holotype: ZHKU 220029

Associated with leaf spot of Canna indica . Sexual morph: not observed. Asexual morph: Conidiophores (18–)20– 40(–78) × 2–4(–5) µm (x = 31 × 3 µm, n = 50), 0–4 septate, pale brown. Conidiogenous cells (4–)7–15(–20) × (2–)4–5 µm (x = 10 × 4 µm, n = 50), pale brown, terminal and lateral. Conidia (17–)30–50(–70) × 3–5 µm (n = 50), pale brown, clavate, guttulate, apex subobtuse, base long obconically subtruncate, 0–3(–6) septa (5 septa not observed), the septa are slightly constricted, some are slightly curved. Spermatogonia forming on OA, 510–770 × 540–770 µm (x = 648 × 653 µm, n = 20), black dots, semi-immersed, globose. Spermatia 2.5–4.5 × 1.5–2 µm (x = 3 × 2 µm, n = 50), smooth, nearly cylindrical.

Cultural characteristics: Colonies on MEA reaching 27 mm diam after one month, flourishes aerial mycelium and smooth, surface white-grey; reverse dark green. Colonies on OA reaching 25 mm diam after one month at 25 ˚C, spreading with moderate aerial mycelium and smooth, surface Brown, reverse olivaceous-grey.

Material examined: CHINA, Guangdong Province, Guangzhou, Haizhu National Wetland Park, on fresh leaves of Canna indica , 4 October 2021, Jieying Lin , (dried culture ZHKU 220029 holotype; ZHKU 220030 , ZHKU 220031 paratype), living cultures ZHKUCC 220038, ex-type; ZHKUCC 220039, ZHKUCC 220040 ex-paratype .

Notes: Three isolates obtained in the present study clustered with Madagascaromyces species in the phylogenetic analysis ( FIGURE 1 ). Our isolate differs from Madagascaromyces intermedius by the size of the conidia and the number of septa ( TABLE 3 ). Madagascaromyces intermedius develop conidia with 1–8 septa, while our isolate Madagascaromyces cannae has 0–6 septa (5 septa not observed), mostly 0–3 septa. Conidia sizes of our isolate (22–45 × 4 µm) are shorter and wider than Madagascaromyces intermedius [(35–)50–75(–100) × (2.5–)3 µm]. Based on a polyphasic approach, M. intermedius is identified as a new species.

Figures

FIGURE 1. Bayesian posterior probabilities tree derived from analysis of a combined LSU, ITS, and rpb2 sequence dataset. Bootstrap support values for Bayesian posterior probabilities (BYPP) greater than 0.95 and maximum likelihood (ML) greater than 75 % and maximum parsimony (MP) greater than 75 % are given at the nodes. Dothiora ceratoniae (CBS 477.69) taxa was used as the outgroup. Strain numbers are given after the species names. The new species identified are highlighted in red. Ex-type strains are in bold.

FIGURE 2. Madagascaromyces cannae (ZHKU 220029 Holotype): a, b. Specimen observed. c, d. Colonies on MEA at 25 °C (b from the bottom). e. Spermatogonium forming on OA. f. Spermatia. g, h. Conidiophores. i, j. Conidiogenous cells. k–p. Conidia. Scale bars = 10 µm.