Entocybe roseoalbus Y. Y. Shen & Y. B. Song

Shen, Yu-Yu, Zhang, Zi-Wen, Li, Wen-Qian, Liu, Xing-Ning, Tian, Fei-Ying, Pang, Chun-Mei, Dai, Wen-Hong, Song, Yao-Bin & Dong, Ming, 2024, Morpho-molecular analysis of two new species Deconica and Entocybe in Agaricales from Mount Tianmu, China, MycoKeys 109, pp. 319-336 : 319-336

Entocybe roseoalbus Y. Y. Shen & Y. B. Song sp. nov.

Figs 5 , 6

Etymology.

The specific epithet roseoalbus (Lat.) refers to the pinkish-white stipe.

Holotype.

• China, Zhejiang Province, Hangzhou, National Nature Reserve of Mount Tianmu at 1025 m a. s. l., 30 ° 20.4 ' N, 119 ° 26.4 ' E ( DDM), grew on humus under coniferous and broad-leaved mixed forest, 2 September 2022, 3461 (holotype), GenBank accessions: PP 974446 ( ITS) and PP 974447 ( LSU).

Description.

Pileus umbonate, undulating, occasional dehiscence in the middle, and slight dehiscence at the edge when mature, not hygrophanous, not translucent-striate, surface finely felted with densely appressed-fibrillose or matted-fibrillose, rivulose, blackish blue (20 F 7 or 20 F 8) in the middle, gradually lighter, becoming dark blue (20 E 6), 47–68 mm diam. Context white, 1.8–1.9 mm thick above the stipe. Lamellae unequal, adnate, margin slightly serrate, 23–26 × 9.3–11.2 mm (length × breadth), at first pinkish white (7 A 2) then pastel red (7 A 4) to pale red (7 A 3) with basidiospore maturity. Stipe central, 69–72 mm long, 7.2–7.7 mm (apex) – 7.1–7.5 mm (middle) – 8.3–8.8 mm (base) diam, equal but slightly thinner in the upper middle, hollow and splits longitudinally with ease, pinkish white (10 A 2), white at the base, fragile. Odor not distinctive. Taste not recorded.

Basidiospores distinctly angular (6–8 angled) to some indistinctly and faintly rounded pustulate, ornamentation composed of broken ridges under an SEM, (4.0) 4.73–5.6 (6.5) × (4.6) 4.8–5.5 (6.1) µm, Q = 0.7–1.3, Q av = 1.0 in side-view. Basidia clavate, 3 – or 4 – sterigmate, filled with refractive oil bodies, 22.3–32.8 × 7.1–9.9 µm. Hymenial cystidia absent. Hymenophoral trama subregular, made up of cylindrical to slightly inflated elements, 36.9–92.5 × 11.6–22.8 µm. Lamella edge crowded with tufts of cheilocystidia. Cheilocystidia clavate, hyaline, abundant, 12.7–25.1 × 2.3–7.1 µm. Hymenial cystidia absent. Pileipellis multi-layered cutis, cylindrical, pigments intracellular, with special long and curved hyphae, 30.2–60.8 × 8–20.9 µm. Pileocystidia (terminal cells) narrowly cylindric to clavate, pigments intracellular, subtended by inflated cells of the pileal trama, 15.7–30.8 × 2.8–4.5 µm. Stipitipellis multi-layered cutis, similar to pileipellis, cylindrical hyphae, pigments intracellular, with special long and curved hyphae, 7.7–12.4 µm diam. Caulocystidia absent. Clamp connections present in all tissues.

Habitat and distribution.

Scatted on humus under coniferous and broad-leaved mixed forests at 1025 m a. s. l., currently only known from Zhejiang Province, China.

Additional material examined

(paratype). • China, Zhejiang Province, Hangzhou, National Nature Reserve of Mount Tianmu at 1025 m a. s. l., 30 ° 20.4 ' N, 119 ° 26.4 ' E ( DDM), scatted on humus under coniferous and broad-leaved mixed forest, 2 September 2022, 3462, GenBank accessions: PP 974445 ( ITS) and PP 974448 ( LSU) .

Figures

Figure 5. Basidiomata of Entocybe roseoalbus (holotype) a habitat of Entocybe roseoalbus b whole basidiomata c lamellae of Entocybe roseoalbus d stipe of Entocybe roseoalbus. Scale bars: 10 mm.

Figure 6. Entocybe roseoalbus (holotype) a marginal cell b basidiospores under the light microscope in oil (1000 ×) c basidiospores under SEM (5200 ×) d – e basidia f – i cheilocystidia j pileocystidia k pileipellis l stipitipellis. Scale bars: 10 µm.