Claviradulomyces vernoniae L.L.Duarte, D.M.Macedo & R.W.Barreto, 2021

Duarte, Lidiane Leal, M, Davi, Macedo, esquita de & Barreto, Robert Weingart, 2021, Cryptic but ubiquitous: Claviradulomyceae fam. nov. with five novel species of the lenticel fungus Claviradulomyces from Brazil, Cryptogamie, Mycologie 20 (7), pp. 121-135 : 131

Claviradulomyces vernoniae L.L.Duarte, D.M.Macedo & R.W.Barreto , sp. nov.

( Fig. 6 )

MYCOBANK NUMBER. — MB 811921.

HOLOTYPE. — Brazil. State of Minas Gerais, Viçosa, campus of Universidade Federal de Viçosa – near the Experimental Barn, on branches of Vernonia sp. ( Asteraceae ), 14.XII.2013, R. W.Barreto

( VIC 42847; ex-type culture COAD 1934; GenBank LSU sequence

KP975455, rpb 2 sequence MW 191753).

ADDITIONAL SPECIMENS EXAMINED. — Brazil. State of Rio de Janeiro, Nova Friburgo, Riograndina, on branches of Vernonia sp. , 11.XII.2013, R. W.Barreto ( VIC 42844). Ibid. 07.IV.2014, R. W.Barreto ( VIC 42839).

ETYMOLOGY. — Refers to its host-genus Vernonia .

DIAGNOSIS. — Similar to C. xylopiae but having a paler subhyaline to yellowish brown pycnidial body instead of dark brown as in C. xylopiae .

HABITAT AND DISTRIBUTION. — Inside lenticels on branches of Vernonia sp. growing in ruderal situations (abandoned pastures and roadsides) at localities in the states of Minas Gerais and Rio de Janeiro ( Brazil).

DESCRIPTION

Asexual morph on living branches bearing abnormal hypertophyed lenticels with spongy tissue. Conidiomata pycnidial, semi-immersed, isolate, subglobose to globose, 75-100 Μm diam, walls subhyaline, textura intrincata at the base, yellowish brown textura angularis and then dark brown at the upper part basal to the neck, ostiolate; neck slightly curved to sinuose, with a broad base, 170-190 Μm long and c. 29 Μm diam, walls dark brown, textura porrecta, progressively tapering towards the apex, ended in acute pale brown tip, smooth, Conidiophores mostly reduced to holoblastic conidiogenous cells, lageniform, straight to slightly curved, 5-7.5 × 1.5-2 Μm, 0-1 septate, hyaline, smooth. Conidia fusoid-filiform to narrowly acerose, straight to curved, 17-35 × 2-3 Μm, attenuated toward the acute tip, subtly heeled at the base ending in narrow stalk, aseptate, guttulate, hyaline, smooth. Sexual morph not observed.

Culture characteristics

Very slow-growing (1.5-2 cm diam after 40 days), colonies grooved or adpressed on the medium, dry or moist, aerial

Figures

FIG. 6. ˗ Claviradulomyces vernoniae sp. nov. (VIC 42847, holotype): A, Vernonia sp. defoliated stem with abnormal lenticels colonized by C. vernoniae; B, closeup of abnormal lenticels; C, D, pycnidium with rostrate ostiole neck; E, detail of conidium. Scale bars: C, D, 30 µm; E, 10 µm.