Neobrevicollum oleae W.L. Li & Jian K. Liu

Lu, Yu-Hang, Zhang, Sheng-Nan, Du, Hong-Zhi, Cheewangkoon, Ratchadawan & Liu, Jian-Kui, 2024, Additions to Crassiparies and Neobrevicollum (Neohendersoniaceae, Pleosporales) associated with woody hosts in Southwest China, Phytotaxa 636 (2), pp. 126-138 : 132

Neobrevicollum oleae W.L. Li & Jian K. Liu , in Mycosphere 14(1): 1495 (2023), Fig. 3 MycoBank: MB 849239

Saprobic on dead branches of Acer palmatum . Sexual morph: Ascomata 240–310 µm high, 350–550 µm diam (x = 280 × 525 µm, n = 10), immersed, solitary, scattered, black, visible as black spots on host surface, globose to subglobose, glabrous, dark brown to black, rough walled, and white interior. Ostiole central, dark brown, with short papillate. Peridium 42–98 µm wide (x = 69 µm), thick, multi-layered, comprising of 5–8 layers of light brown cells of textura angularis. Hamathecium 1.8–2.6 µm wide, numerous, filamentous, cellular pseudoparaphyses, with inconspicuous septa. Asci 72–168 × 14–24 µm (x = 108 × 20 µm, n = 30), 8-spored, hyaline, bitunicate, fissitunicate, cylindrical to clavate, shortly pedicellate (7.2–16.3 µm, n = 30). Ascospores 22.5–30 × 6.5–12 µm (x = 26 × 8.5 µm, n = 50), 1–2- seriate, partially overlapping, hyaline, broadly fusiform, obtuse at both ends, 1-septate, slightly constricted at the septa, the upper cell slightly larger than the lower cell, guttulate, smooth-walled, and surrounded by a mucilaginous sheath, 10.3–19.5 µm wide. Asexual morph: Undetermined.

Culture characteristic:— Colonies on PDA reaching about 20 mm diam. after ten days incubated at 25℃, and about 27 mm diam. after two weeks, circular, flattened, felt-like, sparse, aerial, yellowish-white and becoming grey at the center, the surface smooth with filamentous edge, reverse dark brown at the center and yellowish-white towards the margin.

Material examined: — CHINA, Sichuan province, Chengdu City, Chengdu Botanical Garden, 30°45ʹ55ʺN, 104°7ʹ32ʺE, 530 m elevation, 21 September 2022, on dead branches of Acer palmatum ( Sapindaceae ) in a terrestrial habitat, Y.H. Lu & H.Z. Du, C17A (HUEST 23.0145), living culture UESTCC 23.0145; ibid., HUEST 23.0146, living culture UESTCC 23.0146.

Notes: — Neobrevicollum oleae was introduced by Li et al. (2023) from Olea europaea in Sichuan province, China. Our two collections are morphologically similar to the holotype of N. oleae in having a central ostiole and cylindrical to obclavate asci with an elongate and cylindrical pedicel, and hyaline, fusiform ascospores ( Li et al. 2023). Based on the results of phylogenetic analyses, two isolates (UESTCC 23.0145 and UESTCC 23.0146) grouped with the type strain (CGMCC 3.24430) and the other strain (UESTCC 23.0068) of N. oleae with 100% ML and 1.00 BYPP. Therefore, we identified them as N. oleae and reported the new host record in this study.

Figures

FIGURE 3. Neobrevicollum oleae (HUEST 23.0145) a Substrate. b, c Ascomata on host surface. d, e Vertical section of ascomata. f–i Asci. j Peridium. k Hamathecium. l–q Ascospores. r Germinating ascospore. s Ascospore stained with India ink showing the mucilaginous sheath. t, u Colony on PDA. Scale bars: d, e = 100 µm, f–i = 20 µm, j = 20 µm, k–r = 10 µm, s = 50 µm.