Pareuzebyella sediminis, Huang & Wei & Lai & Chen & Yuan, 2021

Huang, Zhaobin, Wei, Xiaomei, Lai, Qiliang, Chen, Shiyong & Yuan, Jianjun, 2021, PareUZebyella sediminis gen. nov., sp. nov., a novel marine bacterium in the family FlaVObaCTeriaCeae, isolated from a tidal flat sediment, International Journal of Systematic and Evolutionary Microbiology (004606) 71 (1), pp. 1-9 : 8

DESCRIPTION OF PAREUZEBYELLA SEDIMINIS SP. NOV.

Pareuzebyella sediminis (se.di'mi.nis. L. gen. n. sediminis of sediment).

Cells are approximately 0.5 µm in width and 1.6 µm in length. Colonies on MB agar plate are light-orange, round, and small. Growth occurs at the temperature of 15–40 °C with optimum of 30–35 °C. No growth was found at 10 or 45 °C. NaCl requirement range was found to be 0.5–10%, with optimum of 1–3%. The species can degrade Tween 40 and Tween 60, but showed no ability to degrade skim milk (protein), cellulose, starch, and Tween 80. Nitrate cannot be reduced into nitrite. Positive for alkaline phosphatase, esterase (C4), esterase lipase (C8), lipase (C14), leucine arylamidase, valine arylamidase, cystine arylamidase, acid phosphatase, naphthol-AS-BI-Phosphohydrolase, β -galactosidase and α -glucosidase; weak positive for α -mannosidase and tryptophan deaminase, and negative for trypsin, α -chymotrypsin, α -galactosidase, β -glucuronidase, β -glucosidase, N-acetylβ -glucosaminidase, and β -fucosidase. Gelatin and aesculin can be hydrolysed. Voges-Proskauer reaction is positive. The predominant menaquinone is MK-6. The predominant fatty acids are C 15:0 iso, C 17:0 iso 3-OH, C 15:1 iso G, and summed feature 9 (C 17:1 iso ω 9 c and/or C 16:0 10-methyl). The polar lipid profiles included phosphatidylethanolamine (PE), four unidentified aminolipids (AL) and four unidentified lipids. The genome size is 4.9–5.0 Mb with genomic G+C content of 41.5 mol%.

The type strain, S2-4-21 T (= MCCC 1 K03818 T = KCTC 72152 T) was isolated from the sediment of coastal cordgrass S. alterniflora and another strain MT2-5-19 (= KCTC 72539 = MCCC 1 K03874 ) was isolated from the neighbouring oyster field in Quanzhou bay , China .

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strain S2-4-21 T and MT2-5-19 are MN227660 and MN227661 , respectively. The whole genome shotgun projects of strain S2-4-21 T and MT2-5-19 has been deposited at DDBJ/ENA/GenBank under the accessions of VTZS00000000 and VTZT00000000, respectively.