Diaporthe kadsurae C.M. Tian & Q. Yang

Yang, Qin, Fan, Xin-Lei, Guarnaccia, Vladimiro & Tian, Cheng-Ming, 2018, High diversity of Diaporthe species associated with dieback diseases in China, with twelve new species described, MycoKeys 39, pp. 97-149 : 97

Diaporthe kadsurae C.M. Tian & Q. Yang sp. nov. Figure 14

Diagnosis.

Diaporthe kadsurae differs from its closest phylogenetic species D. fusicola and D. ovoicicola in ITS, cal and tef1 loci based on the alignments deposited in TreeBASE.

Holotype.

CHINA. Jiangxi Province: Shangrao city, Sanqing Mountain, on symptomatic branches of Kadsura longipedunculata , 1 Apr. 2017, B. Cao, Y.M. Liang & C.M. Tian (holotype: BJFC-S1497; ex-type culture: CFCC 52586).

Etymology.

Named after the host genus on which it was collected, Kadsura .

Description.

Conidiomata pycnidial, immersed in bark, scattered, slightly erumpent through the bark surface, nearly flat, discoid, with a single locule. Ectostromatic disc obviously, brown to black, one ostiole per disc. Locule undivided, 475-525 μm diam. Conidiophores 7-11 × 1.8-2.9 μm, cylindrical, hyaline, unbranched, straight or slightly curved, tapering towards the apex. Alpha conidia hyaline, aseptate, oval or fusoid, biguttulate, 5.5-7.5 × 2.1-2.9 μm (av. = 6.5 × 2.5 μm, n = 30). Beta conidia not observed.

Culture characters.

Cultures incubated on PDA at 25 °C in darkness. Colony originally flat with white aerial mycelium, becoming dense and felted aerial mycelium in the centre and grey to black mycelium at the marginal area with solitary conidiomata at maturity.

Additional specimens examined.

CHINA. Jiangxi Province: Shangrao city, Sanqing Mountain, on symptomatic branches of Kadsura longipedunculata , 1 Apr. 2017, B. Cao, Y.M. Liang & C.M. Tian, living culture CFCC 52587 (BJFC-S1498); Yunbifeng National Forest Park, on symptomatic twigs of Acer sp., 31 Mar. 2017, B. Cao, Y.M. Liang & C.M. Tian, living culture CFCC 52588 (BJFC-S1499); ibid. living culture CFCC 52589 (BJFC-S1500).

Notes.

This new species is introduced as molecular data show it to be a distinct clade with high support (ML/BI=100/1) and it appears most closely related to D. fusicola and D. ovoicicola . Diaporthe kadsurae can be distinguished from D. fusicola by 11 nucleotides in concatenated alignment, in which 4 were distinct in the ITS region and 7 in the cal region; from D. ovoicicola by 25 nucleotides in concatenated alignment, in which 12 were distinct in the ITS region, 6 in the cal region and 7 in the tef1 region. Morphologically, D. kadsurae differs from D. fusicola and D. ovoicicola in shorter conidiophores (7-11 μm in D. kadsurae vs. 11-24.1 μm in D. fusicola ; 7-11 μm in D. kadsurae vs. 14.2-23.6 μm in D. ovoicicola ) ( Gao et al. 2014).