Saccharopolyspora rhizosphaerae, Intra & Euanorasetr & Také & Inahashi & Mori & Panbangred & Matsumoto, 2019

Intra, Bungonsiri, Euanorasetr, Jirayut, Také, Akira, Inahashi, Yuki, Mori, Mihoko, Panbangred, Watanalai & Matsumoto, Atsuko, 2019, Saccharopolyspora rhizosphaerae sp. nov., an actinomycete isolated from rhizosphere soil in Thailand, International Journal of Systematic and Evolutionary Microbiology 69 (5), pp. 1299-1305 : 1303

DESCRIPTION OF SACCHAROPOLYSPORA RHIZOSPHAERAE SP. NOV.

Saccharopolyspora rhizosphaerae (rhi.zo.sphae′ rae. Gr. n. rhiza root; L. n. sphaera a ball, sphere; N.L. gen. n. rhizosphaerae of rhizosphere).

Aerobic, Gram-positive, catalase-positive and oxidasepositive. The colonies are pale yellow in colour with white aerial mycelia that produce spherical-shaped, warty and non-motile spores. Soluble pigments are not produced. Growth of the type strain is observed at temperatures of between 18 and 37 Ǫ C (optimum, 22–36 Ǫ C), at pH 6–11 and in the presence of up to 14 % NaCl (w/v). The organism can utilise D- arabinose, fructose, glucose, mannitol, myo -inositol, L- rhamnose, sucrose and xylose as the sole carbon source, but cannot use melibiose and raffinose. Acid is produced from glucose, L- rhamnose, sucrose and xylose. L- Alanine, L- histidine, L- phenylalanine, L- proline, L- serine, L- tyrosine, L- valine and methionine can be utilised as the sole nitrogen source, but adenine, glycine and L- glutamic acid are not utilised. The strain is positive for hydrogen sulphide production, gelatin liquefaction, casein hydrolysis, milk peptonisation and starch hydrolysis, but negative for melanin production and milk coagulation. The cell wall contains meso - DAP. Arabinose and galactose are found in whole-cell hydrolysates. The major types of cellular fatty acids are iso-C 16: 0, anteiso-C 17: 0, iso-C 15: 0, and 10-methyl C. The muramic acid is acetyl type and no mycolic acids are detected. The polar lipids are diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylethanolamine-containing hydroxylated fatty acids and an unknown phospholipid. MK-9(H 4) is the predominant menaquinone.

17: 0

The G+C content of the genomic DNA is 70.6 mol%. The type strain is H219 T (=TBRC 8564 T =NBRC 113388 T).