Paraneoaraneomyces sinensis Zhi. Y. Zhang & Y. F. Han, sp. nov.

Zhang, Zhi-Yuan, Feng, Yao, Tong, Shuo-Qiu, Ding, Chen-Yu, Tao, Gang & Han, Yan-Feng, 2023, Morphological and phylogenetic characterisation of two new soil-borne fungal taxa belonging to Clavicipitaceae (Hypocreales, Ascomycota), MycoKeys 98, pp. 113-132 : 113

Paraneoaraneomyces sinensis Zhi. Y. Zhang & Y. F. Han, sp. nov.

Fig. 2

Etymology.

After the country of origin.

Type.

Kaili City , Guizhou Province, China; 27°17 ’56’’ N, 108°82 ’68’’ E; isolated from the green belt soil in July 2022; Zhi-Yuan Zhang (holotype ZY H-22.006, ex-holotype ZY 22.006, ibid., ZY 22.007) .

Geographical distribution.

Guizhou Province, China.

Description.

Culture characteristics (14 days at 25 °C): Colonies on PDA 35-37 mm in diameter, white, slightly raised at centre, fluffy, nearly round, margin regular; reverse: pale yellow. Colonies on MEA 35-37 mm in diameter, white, plicated, flocculent, nearly round, margin regular; reverse: pale yellow. Colonies on SNA 29-31 mm in diameter, white, flat, felty, nearly round, margin regular; reverse: white, compact at centre. Colonies on OA 36-38 mm in diameter, white, felty, early round, margin regular; reverse: white.

Hyphae hyaline, smooth, branched, septate, 1.0-3.0 μm in diameter. Phialides arising from aerial hyphae or hyphae regimental, solitary, straight to flexuous, tapering with enlarged base, smooth, hyaline, 19.0-34.0 × 0.5-1.5 µm (av. 27.0 × 1.1, n = 50). Conidia borne on the apices of the phialides or in small globose heads at the apex of the phialides. Conidia cymbiform to reniform, smooth-walled, one-celled, adhering in globose heads or the apex of phialides, 3.0-5.5 × 1.0-1.5 µm (av. 4.3 × 1.4, n = 50). Sexual morph undetermined.

Additional material examined.

Kaili City, Guizhou Province, China ; 27°17 ’72’’ N, 108°83 ’10’’ E; isolated from the green belt soil in July 2022; Zhi-Yuan Zhang, ZY 22.008.

Notes.

The multi-locus phylogenetic analyses showed that Paraneoaraneomyces sinensis is closely related to Neoaraneomyces araneicola (Fig. 1 ), but can be distinguished, based on differences in their sequence similarity. The ITS sequence of P. sinensis showed 93.6% similarity, differences in 13 base pairs (bp) and 22 gaps when compared to the 551 bp ITS sequence of N. araneicola DY101711 (Type strain). The LSU sequence of P. sinensis showed 99.3% similarity, differences in 5 bp and without gaps when compared to the 832 bp LSU sequence of N. araneicola DY101711. The RPB2 sequence of P. sinensis showed 83.9% similarity, differences in 158 bp and 8 gaps when compared to the 1,034 bp RPB2 sequence of N. araneicola DY101711. The EF1A sequence of P. sinensis showed 96.2% similarity, differences in 35 bp and without gaps when compared to the 937 bp EF1A sequence of N. araneicola DY101711. Morphologically, the phialides of P. sinensis were solitary, straight to flexuous, arising from the aerial or regimental hyphae compared to the phialides of N. araneicola that were solitary or in groups of two to four and arose from the aerial hyphae ( Chen et al. 2022). Furthermore, the conidia of P. sinensis were cymbiform to reniform and adhering to the apex of the phialides or in small globose heads at the apex of the phialides compared with fusiform to ellipsoidal conidia that were arranged as chains in N. araneicola ( Chen et al. 2022).

Figures

Figure 1. Phylogram based on the Maximum Likelihood (ML) analysis using the SSU, ITS, LSU, RPB 2 and EF 1 A sequences of Clavicipitaceae. The ML bootstrap values (≥ 70 %) and the Bayesian posterior probability values (≥ 0.70) are indicated along the branches (BP / ML). The new taxa are highlighted in bold.

Figure 2. Morphology of Paraneoaraneomyces sinensis sp. nov. a-d colony on PDA, MEA, SNA and OA after 14 d at 25 ° C (upper surface and lower surface) e-h phialides, conidia i-k phialides are arising from hyphae regimental, conidia. Scale bars: 10 μm (e-k).