Curvicladiella paphiopedili Lian-Chai Song，Jing Yang, Zuo-Yi Liu, 2019

Song, Lian-chai, Huang, Lu, Liu, Ling-ling, Feng, Yao, Wang, Li-li & Liu, Zuo-yi, 2022, Curvicladiella paphiopedili sp. nov. (Hypocreales, Nectriaceae), a new species of orchid (Paphiopedilum sp.) from Guizhou, China, Biodiversity Data Journal 10, pp. 80122-80122 : 80122

Curvicladiella paphiopedili Lian-Chai Song，Jing Yang, Zuo-Yi Liu, 2019 sp. nov.

Materials

Type status: Holotype. Occurrence: recordNumber: zwy-dl4-2; recordedBy: Lian Chai Song; Taxon : scientificName: Curvicladiella paphiopedili; class: Sordariomycetes ; order: Hypocreales ; family: Nectriaceae ; genus: Curvicladiella ; Location : locationRemarks: China, Gui Zhou Province , Guiyang City , Guizhou Botanical Garden , 26°37'N, 106°43'E, 13 August 2019; Event: habitat: Terrestrial; fieldNotes: diseased leaves of Paphiopedilum sp.; Record Level: type: Stilllmage; language: English; collectionID: MFLU 20-0203 Type status: Isotype. Occurrence: recordNumber: zwy-dl4-2; recordedBy: Lian Chai Song; Taxon : scientificName: Curvicladiella paphiopedili; class: Sordariomycetes ; order: Hypocreales ; family: Nectriaceae ; genus: Curvicladiella ; Location : locationRemarks: China, Gui Zhou Province , Guiyang City , Guizhou Botanical Garden , 26°37'N, 106°43'E, 13 August 2019; Event: habitat: Terrestrial; fieldNotes: diseased leaves of Paphiopedilum sp.; Record Level: type: Stilllmage; language: English; collectionID: GZAAS 19-2061

Description

The characters of pathogenic fungi on the leaves were identified through an artificial infection test. Asexual morph: Conidiomata white, scattered, hairy. Conidiophores straight to flexuous, consisting of a stipe bearing a penicillate arrangement of fertile branches, stipe septate, hyaline, smooth; stipe extensions septate, straight or curved, dull and tapering towards the apex, 128.5-549.9 µm long, (x̄= 288.1 µm, n = 20). The primary branches of conidiogenous apparatus aseptate, 9.3-17.5 × 2.6-3.7 μm; secondary branches aseptate, 9.9-19.1 × 2.5-3.9 μm; tertiary branches aseptate, 9.5-17.6 × 2.6-3.7 μm; quaternary and additional branches (-6) aseptate, 11-16.3 × 2.5-3.9 μm, each terminal branch producing 2-4 phialides; phialides doliiform to reniform, hyaline, aseptate, apex with minute periclinal thickening and inconspicuous collarette. Conidia cylindrical, rounded at both ends, straight, 1-septate, hyaline, (30.5-) 31.2-37.2 (-42.0) × (2.6-) 2.9-3.5 (-3.9) µm, (x̄= 34.2 × 3.2 µm, n = 20) (Fig. 1 ). Sexual morph: not observed.

The characters of fungus obtained from the diseased leaves collected in the field that were cultured with PDA: after 10 days at 25°C on PDA, colonies reached 47 mm in diam. Beige to pale yellow colony on the surface, brown in reverse with irregular margins, extensive sporulation on the medium surface. Conidiophores straight to flexuous, consisting of a stipe bearing a penicillate arrangement of fertile branches, stipe extensions septate, straight or slightly flexuous, 104.4-153.0 µm long, (x̄= 128.7 µm, n = 10). The primary branches of conidiogenous apparatus aseptate, 8.9-17.8 × 2.7-3.4 μm; secondary branches aseptate, 7.8-14.0 × 2.5-5.9 μm; tertiary branches aseptate, 8.9-17.7 × 2.3-3.5 μm; quaternary and additional branches (-6) aseptate, 9.3-16.7 × 2.3-3.7 μm, each terminal branch producing 2-4 phialides; phialides doliiform to reniform, hyaline, aseptate, apex with minute periclinal thickening and inconspicuous collarette. Conidia cylindrical, rounded at both ends, straight, 1-septate, hyaline, (38.5-) 45.2-56.6 (-63.2) × (2.2-) 2.9-4.2 (-4.9) µm, (x̄= 50.9 × 3.5 µm, n = 40). Chlamydospores thick-walled, ellipsoidal or sphaeropedunculate, brown to hyaline, (9.0-) 11.9-20.7 (-23.1) × (8.1-) 8.9-12.8 (-15.4) µm, (x̄= 16.3 × 10.8 µm, n = 20) (Fig. 2 ).

Material: ex-type living culture, MFLUCC 20-0110.

Etymology

Refers to the genus name Paphiopedilum .

Figures

Figure 1. Curvicladiella paphiopedili. a The diseased leaves were withered; b, c Conidiomata; d-g Stipes extension and conidiogenous cells; h-j Conidiogenous cells and conidiophores; k-n Conidia. Scale bars: d-g = 50 µm, h-j = 20 µm, k-n = 10 µm.

Figure 2. Curvicladiella paphiopedili. (MFLU 20 - 0203, holotype) a Paphiopedilum diseased leaf in the field; b The healthy leaves diseased after inoculating the mycelial PDA plug of Curvicladiella paphiopedili; c The healthy leaves did not become infected after being inoculated with free PDA plug as control; d Colonies on PDA producing conidia masses; e-j Conidiophores, conidiogenous cells and stipes extension; k Chlamydosporae; l, m Conidia; n, o Culture on PDA; (n) from above, (o) from below. Scale bars: e-g = 50 µm, h-k = 20 µm, l, m = 10 µm.