Graphilbum kesiyae R.Chang & Z.W.de Beer

Chang, Runlei, Duong, Tuan A., Taerum, Stephen J., Wingfield, Michael J., Zhou, Xudong & Beer, Z. Wilhelm de, 2017, Ophiostomatoid fungi associated with conifer-infesting beetles and their phoretic mites in Yunnan, China, MycoKeys 28, pp. 19-64 : 32

Graphilbum kesiyae R.Chang & Z.W.de Beer sp. nov. Fig. 9

Etymology.

The epithet kesiyae refers to the tree host of all beetles and mites from which the 12 isolates of this species were collected.

Description.

Sexual state not observed. Pesotum -like macronematal asexual states predominant. Synnemata simple, dark brown at the base, (85.5-) 112.5-173 (-203) μm long including conidiogenous apparatus, (9-) 14-45.5 (-65.5) μm wide at base; cells (8.5-) 10-18.5 (-25.5) μm long; conidia hyaline, 1-celled, smooth, oblong, (3.5-) 4-5 (-5.5) × (1.5-) 1.5-2 (-2.5) μm. Hyalorhinocladiella-like asexual state: conidiophores (22-) 38-101.5 (-166) μm long; cells arising directly from the hyphae, (10-) 12 –27(– 40) × (1.2-) 1.5-2 (-2.5) μm; conidia hyaline, smooth, obovoid, (3.5-) 4-5.5 (-8.5) × (1-) 1.5-2 (-3) μm.

Culture characteristics.

Colonies hyaline. Mycelium superficial on the 3% OA. Colony margin smooth. Colonies on 2% MEA flat, reaching 85 mm diam in 10 d at 25 °C. No growth observed at 5 and 35 °C. Optimal temperature for growth 25 °C.

Type material.

CHINA, Yunnan Province, Puer City, from Polygraphus szemaoensis gallery on Pinus kesiya bark, 12 Aug. 2010, S.J.Taerum, herbarium specimen of dried culture, PREM 61541 (holotype), CMW41729 = CBS139652 (ex-holotype culture).

Additional specimens examined.

CHINA, Yunnan Province, Puer City, from Insectolaelaps sp. 1 in Polygraphus szemaoensis gallery on Pinus kesiya bark, 10 Aug. 2010, S.J.Taerum, CMW41691 = CBS139642; CHINA, Yunnan Province, Puer City, from Proctolaelaps nr. hystrix in Polygraphus szemaoensis gallery on Pinus kesiya bark, 11 Aug. 2010, S.J.Taerum, PREM 61542, CMW41716 = CBS139657.

Host.

Pinus kesiya .

Beetle vectors.

Polygraphus aterrimus , Polygraphus szemaoensis .

Mite vectors. Proctolaelaps nr. hystrix (phoretic on Polygraphus szemaoensis ), Insectolaelaps sp. 1 (phoretic on Polygraphus szemaoensis ).

Distribution.

At present known only from Yunnan, China.

Notes.

Graphilbum kesiyae and Gra. puerense can be distinguished from Gra. crescericum by the presence of both synnematous and hyalorhinocladiella-like asexual states in culture. Gra. crescericum produces only the hyalorhinocladiella-like asexual state. The optimal temperature for growth of Gra. puerense is 30 °C while that for Gra. kesiyae is 25 °C, and synnemata of Gra. puerense reach double the length of those of Gra. kesiyae .