Calcarisporium guizhouense W. H. Chen, Y. F. Han & J. D. Liang, 2024

Chen, Wan-Hao, Li, Dan, Liang, Jian-Dong, Ren, Xiu-Xiu, Zhao, Jie-Hong & Han, Yan-Feng, 2024, Chlorocillium sinense sp. nov. (Clavicipitaceae) and Calcarisporium guizhouense sp. nov. (Calcarisporiaceae) in Hypocreales from China, MycoKeys 109, pp. 91-107 : 91-107

Calcarisporium guizhouense W. H. Chen, Y. F. Han & J. D. Liang sp. nov.

Fig. 3

Etymology.

Referring to its type location in Guizhou Province.

Type.

China • Guizhou Province, Qiannan Buyei and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E), on Cordyceps sp. , 1 May 2022, Wanhao Chen, GZAC DY 0504 (holotype), ex-type DY 05041 .

Description.

Colonies on PDA attaining a diameter of 28–29 mm after 14 days at 25 ° C, white, consisting of a basal felt, floccose hyphal overgrowth, yellowish-white; reverse light brown to brown. Hyphae septate, hyaline, smooth-walled, 2.0–2.2 μm wide. Conidiophores erect, hyaline, verticillately branched, with 1–3 conidiogenous cells. Conidiogenous cells 15.6–23.2 × 1.5–1.7 μm, hyaline, cylindrical at base, gradually tapering near the apex, holoblastic to polyblastic, sympodial, apically with a cluster of conidium-bearing denticles. Conidia 5.2–8.6 × 1.8–2.2 μm, hyaline, smooth-walled, thin-walled, cylindrical, unicellular, acuminate.

Substrate.

Cordyceps sp.

Additional strain examined.

China • Guizhou Province, Qiannan Buyei and Miao Autonomous Prefecture, Duyun City, Mayao River Valley (26 ° 22 ' 8.3748 " N, 107 ° 23 ' 16.96 " E). On Cordyceps sp. , 1 May 2022, Wanhao Chen, DY 05042 (living culture) .

Notes.

Calcarisporium guizhouense was easily identified as Calcarisporium , based on the BLASTn result in NCBI and its verticillate conidiophores and sympodial conidiation. Phylogenetic analyses show that Calcarisporium guizhouense has close relationships to C. arbuscula and C. yuanyangense (Fig. 1 ). However, C. guizhouense was easily distinguished from C. arbuscula (Conidia: ovoid to ellipsoid, 4–11 × 1.8–3.3 μm; substrate: decaying agaric) and C. yuanyangense (Conidia: ovoid to ellipsoid, 6.33–9.68 × 1.87–2.63 μm; substrate: Ophiocordyceps nutans ) by its smaller cylindrical conidia and the substrate. Thus, the morphological characteristics and molecular phylogenetic results support C. guizhouense as a new species.

Figures

Figure 1. Phylogenetic analysis of the new strains and Chlorocillium species in the order Hypocreales based on multigene dataset (ITS, LSU, RPB 2 and tef - 1 α). Statistical support values (≥ 50 % / 0.5) are shown at the nodes for ML bootstrap support / BI posterior probabilities. The new taxa are in bold.

Figure 3. Calcarisporium guizhouense A the substrate Cordyceps sp. (fungicolous) B, C PDA culture plate showing top (B) and reverse (C) sides of the colony D – K Conidiogenous cells and conidia. Scale bars: 10 mm (B, C); 10 μm (D – K).