Phyllonorycter, Hübner, 1822

Kirichenko, Natalia, Triberti, Paolo, Akulov, Evgeniy, Ponomarenko, Margarita, Gorokhova, Svetlana, Sheiko, Viktor, Ohshima, Issei & Lopez-Vaamonde, Carlos, 2019, Exploring species diversity and host plant associations of leaf-mining micromoths (Lepidoptera: Gracillariidae) in the Russian Far East using DNA barcoding, Zootaxa 4652 (1), pp. 1-55 : 33

Phyllonorycter sp. 8 ( cf. pterocaryae Kumata )

( Figs 14 B–C)

Material examined. Russia: PK, Gornotaezhnoe , forest around MTS, 43.68N, 132.15E, 224 m alt., Juglans mandshurica , 23.VII.2016, 1 larva, NK533 , MK 403680 , deposited in INRA.

Leaf mine. The mine is a small oval silvery white blotch, slightly contracted in the late stage, usually without preceding epidermal tunnel; situated at the leaf margin on the upper side of the leaf ( Fig. 14B ). Pupation in the mine, at the corner of the mine, in a slight depression similar to that observed in Phyllocnistis ( Fig. 14C ).

Trophic specialization. Monophagous on Juglans mandshurica (Juglandaceae) .

Distribution. Russia: RFE—PK.

Remarks. BIN of unknown species—BOLD: ADF4351. DNA barcode of Phyllonorycter sp. 8 is 3.1% divergent from the DNA barcodes of four Ph. pterocaryae (Kumata) specimens collected in Japan on J. mandschurica , whose identity was confirmed by adult morphology (Ohshima: personal observation). The fact that Phyllonorycter sp. 8 larvae feed on the same host as Ph. pterocaryae and make similar mines suggests that our finding may correspond to Ph. pterocaryae , which might have high intraspecific genetic variability. According to early records, Ph. pterocaryae is known in the southern part of Primorskii Krai ( Baryshnikova 2008, 2016). In BOLD, there are three other BINs corresponding to Phyllonorycter feeding on Juglandaceae : the two unidentified species on Platycarya ( Taiwan) , Pterocarya ( Vietnam), and one Phyllonorycter juglandis ( Japan) . Phyllonorycter sp. 8 matches none of these species, showing the divergence from them: 4.4, 7.5 and 11.8%, respectively ( Table 2 ).

Figures

FIGURE 14. Mines, leaf shelters and pupation sites of Phyllonorycter, Chrysaster and Cameraria spp. from the Russian Far East. (A) Phyllonorycter sp. 7, host plant Ulmus glabra; (B–C) Phyllonorycter sp. 8, Juglans mandshurica; (D–E) Chrysaster hagicola, Lespedeza bicolor; (F–G) Cameraria niphonica, Acer pseudosieboldianum and A. caudatum subsp. ukurundense, respectively; (H) Phyllocnistis sp. 1, Salix sp. Indications: (m) mine; (b) blotch part of the mine; (t) epidermal tunnel; (l) larva; (p) pupation site. Close up: (C–G) mine; (H) pupation site. Sampling locations: (A) SO, Sakhalin Isl., Yuzhno-Sakhalinsk, 11–20.VII.2017; (B–H) PK, Gornotaezhnoe, MTS and forest, 22–26.VII.2016.