Coprinopsis jilinensis G. Rao, H.N. Zhao, B. Zhang & Y. Li, 2021

Rao, Gu, Dai, Dan, Zhao, Hui-Nan, Liang, Yi, Li, Yu & Zhang, Bo, 2021, Two new psathyrelloid species of Coprinopsis (Agaricales, Psathyrellaceae) from China, MycoKeys 83, pp. 85-103 : 85

Coprinopsis jilinensis G. Rao, H.N. Zhao, B. Zhang & Y. Li sp. nov.

Figures 3 , 4 , 7C, D

Typification.

China. Red Leaves Valley in Hanchongling, Dunhua City, Yanbian Korean Autonomous Prefecture, Jilin Province, 22 August 2019, G. Rao & H.N Zhao (HMJAU 58782 Holotype!).

Sequences ex holotype.

MZ398015 (ITS nrDNA), MZ398070 (LSU nrDNA).

Etymology.

The epithet " Coprinopsis jilinensis " refers to this species that was first discovered in Jilin Province, China.

Description.

Basidiomata small to medium-sized. Pileus 33-52 mm broad, conical to convex, dark brown or clay brown, densely covered with white hairs, not sticky when dry or wet, not hygrophanous, veil remnants flocculent at edges. Lamellae close or crowded, grey-white to fleshy brown, brownish-black after drying, sinuate or adnexed, not the same length and width, edges slightly toothed, concolorous, not deliquescent. Stipe 80-95 × 5-9 mm, white to milky white, cylindrical, down slightly rough, fibrous, a little fragile, hollow, the base with white mycelium, dense or sparse, close to the stipe surface covered with brownish-yellow pubescent, no ring. Spore print without record.

Basidiospores [60, 2, 2] (8)8.5-10(10.2) × 4.5-5.9 (6) µm, avl = 9.1 µm, avw = 5.2 µm, Q = (1.62) 1.63-1.96 (2.02), Qm = 1.77 ± 0.09, oval to long oval, brown, brownish-yellow or dark brown in 5% KOH solution, smooth, thick wall, dextrinoid, apical with small pores, 1-2 µm. Basidia 17-30 (39) × 8-10 (13) µm, clavate, 4-sterigmate up to 3-4 µm long, 2-3 sterigmate occasional. Pleurocystidia (30) 33-59 (60) × (11) 12-21 (23) µm, utriform and lageniform, sparse, smooth, hyaline. Cheilocystidia 27-56 × (10) 11-20 (22) µm, utriform and lageniform, smooth, hyaline, crowded in hymenium. Lamellar edge fertile. Pileipellis a cutis, up to 100 µm thick, hyphae (35) 42-111 (148) × (6) 7-34 (35) µm, ovoid, subcylindrical, with brownish-yellow to dark brown pigment, thick wall, encrusting pigment on the outer hyphae. Veil hyphae (5) 6-30 (33) µm wide, present dark encrusting pigment, thick wall, colourless to light yellow, cylindrical and subcylindrical. Stipitipellis a cutis, hyphae (5) 6-22 (32) µm diam., ovoid and subcylindrical, pale brown, with encrusting pigment, thick wall. Clamp connections present in all tissues.

Habitat and distribution.

On humus of broad-leaved forest or coniferous and broad-leaved mixed forests in autumn.

Additional specimens examined.

China. Red Leaves Valley in Hanchongling, Dunhua City, Yanbian Korean Autonomous Prefecture , Jilin Province, 14 September 2019, Gu Rao (HMJAU 58783) .

Notes.

Coprinopsis jilinensis is characterised by its small to medium-sized basidiomata, brown pileus with white hairs, smooth and dextrinoid basidiospores with small pores, pleurocystidia and cheilocystidia present. C. jilinensis forms a strongly-supported independent clade in ITS and LSU phylogeny trees (Figs 1 , 2 ).

Morphologically and phylogenetic similar to Coprinopsis jilinensis , C. uliginicola is characterised by long basidiospores of 10-12(-15) µm, pleurocystidia absent and caulocystidia present, pileipellis no encrusting pigment ( Smith 1972). Other similar species, C. cineraria is characterised by grey, hygrophanous and striate pileus, little short basidiospores (6.5-8.5 µm), pleurocystidia absent, pileipellis an epithelium ( Takahashi 2000); C. melanthina is characterised by little long and subcolourless basidiospores (avl × avw = 10.5 × 5.8 µm), pleurocystidia absent (Kits van Waveren 1985); C. pusilla has small basidiomata, grey pileus, subcolourless and verrucose basidiospores (this study) and Psathyrella subagraria has hygrophanous pileus, thick flesh and caulocystidia present ( Smith 1972), both of which could be clearly distinguished from C. jilinensis in terms of morphology.

Figures

Figure 1. The phylogenetic tree of Coprinopsis by ITS nrDNA, based on the Maximum Likelihood method (ML). The three values of internal nodes respectively represent Maximum Likelihood bootstrap (MLBP) / neighbour-joining bootstrap (NJBP) / Bayesian posterior probability (BIPP). The thick node indicates the significantly-supported branch in at least two analyses (MLBP ≥ 70, NJBP ≥ 70, BIPP ≥ 95 %). The GenBank accession number is marked after the species name. At the same time, the sequence from the type specimen is also marked at the end. Two new species from China are expressed in bold font and Parasola conopilea (Fr.) Oerstadius & E. Larss and P. auricoma (Pat.) Redhead, Vilgalys & Hopple are selected as the outgroups.

Figure 2. The phylogenetic tree of Coprinopsis by LSU nrDNA, based on Bayesian Inference (BI). The three values of internal nodes respectively represent Maximum Likelihood bootstrap (MLBP) / neighbour-joining bootstrap (NJBP) / Bayesian posterior probability (BIPP). The thick node indicates the significantly-supported branch in at least two analyses (MLBP ≥ 70, NJBP ≥ 70, BIPP ≥ 95 %). The GenBank accession number is marked after the species name. At the same time, the sequence from the type specimen is also marked at the end. Two new species from China are expressed in bold font and Parasola conopilea (Fr.) Oerstadius & E. Larss and P. auricoma (Pat.) Redhead, Vilgalys & Hopple are selected as the outgroups.

Figure 3. Basidiomata and microscopic features of Coprinopsis jilinensis A collection HMJAU 58783 B collection HMJAU 58782 C basidiospores D basidia E pleurocystidia F Cheilocystidia G pileipellis. Scale bars: 5 mm (A, B); 10 µm (C-G).

Figure 4. Scatter plot of basidiospores size in Coprinopsis jilinensis.

Figure 7. Scanning electron micrograph of basidiospores A, B Coprinopsis pusilla C, D C. jilinensis. Scale bars: 2 µm (A-D).