Arecophila miscanthi (Karun., C.H. Kuo & K.D. Hyde) Q.R Li & J.C. Kang, 2022

Li, Qi Rui, Zhang, Xu, Lin, Yan, Samarakoon, Milan C., Hyde, Kevin David, Shen, Xiang Chun, Liao, Wan Qing, Karunarathna, Anuruddha, Long, Si Han, Kang, Ying Qian & Kang, Ji Chuan, 2022, Morpho-molecular characterisation of Arecophila, with A. australis and A. clypeata sp. nov. and A. miscanthi comb. nov., MycoKeys 88, pp. 123-149 : 123

Arecophila miscanthi (Karun., C.H. Kuo & K.D. Hyde) Q.R Li & J.C. Kang comb. nov.

Alishanica miscanthi ≡ Alishanica miscanthi Karun., C.H. Kuo & K.D. Hyde [as ‘miscanthii’], in Hyde et al., Mycosphere 11(1): 461 (2020)

Description

(MFLU 19-2333). Saprobic on dead sheaths of Miscanthus sinensis ( Poaceae ). Sexual morph: Ascomata 272-277 × 283-296 µm (x̄ = 275 × 291.5 µm, n = 8), immersed beneath blackened aggregated clypeus of the surface of dead sheath, loosely aggregated or rarely solitary; dark brown to black, globose to subglobose, slightly depressed, uniloculate. Ostiole 92-110 μm long, 52-56 μm diameter (x̄ = 101 × 54 μm, n = 5), centrally erumpent, with periphyses, surrounded by distinct shiny black flanges, the tissue spreading down along the papilla. Peridium 51-60 μm wide, comprising 4-5 cell layers of thin-walled, brown cells of textura angularis, inwardly lighter. Paraphyses filamentous, distinctly septate, embedded in a hyaline gelatinous matrix. Asci 147-189 × 10-13 μm (x̄ = 167 × 11 μm, n = 30), 8-spored, unitunicate, cylindrical, short pedicellate, slightly truncate at the apex, with a wedge-shaped J+, subapical ring, 3.5-4 µm broad, 2-2.5 µm high. Ascospores 20-24 × 6-8 μm (x̄ = 22 × 7 μm, n = 40), overlapping, uniseriate, ellipsoidal, slightly tapering at the ends, equally 2-celled and guttulate at both cells, constricted at the septum, brown with striations, surrounded by a thick, hyaline mucilaginous sheath, subglobose, parallel to the margin of the spore. Asexual morph: Undetermined.

Material examined.

China, Taiwan, Chiayi Province, Ali Mountain, Kwang Hwa , on dead sheaths of Miscanthus sinensis ( Poaceae ), 5 May 2018, A. Karunarathna, AKTW 44 (MFLU 19-2333, holotype)

Additional material.

China, Yunnan Province, Kunming City, Kunming Botanical Garden (25°8'45"N, 102°44'59"E), on dead culm of monocotyledon, 20 March 2014, Q.R. Li, GZ43 (GZUH0122, GACP QR0201; living cultures, GZUCC0122) .

Note.

The characteristics of the holotype specimen Arecophila miscanthi (≡ Alishanica miscanthi ) were revised, re-measured and described. Alishanica miscanthi is similar to A. muroiana and A. serrulata ( Wang et al. 2004, Hyde et al. 2020). However, no clypeus was observed for A. muroiana . Arecophila serrulata has larger ascomata (480-560 × 280-320 μm) with a central papilla surrounded by a circle of white tissue ( Hyde 1996) which differs from those of A. miscanthi . One new collection (GZUH0122, Fig. 5 ) shows the same traits of A. miscanthi (MFLU 19-2333) in having immersed ascomata with clypeus, a wedge-shaped J+, ascus subapical ring, same dimensions of ascospores and here we provide it as a new geographical record from China.

Figures

Figure 5. Arecophila miscanthi (GZUH 0122) A, B ascomata on the surface of host C paraphyses and asci D section of ascoma E peridium F, G apical rings H-K asci with ascospores M-P ascospores. Scale bars: 50 μm (C, D); 10 μm (E, F-K); 5 μm (L-P).