Distoseptispora bambusae Y.R. Sun, I.D. Goonasekara, Yong Wang bis & K.D. Hyde, 2020

Sun, Yaru, Goonasekara, Ishani D., Thambugala, Kasun M., Jayawardena, Ruvishika S., Wang, Yong & Hyde, Kevin D., 2020, Distoseptispora bambusae sp. nov. (Distoseptisporaceae) on bamboo from China and Thailand, Biodiversity Data Journal 8, pp. 53678-53678 : 53678

Distoseptispora bambusae Y.R. Sun, I.D. Goonasekara, Yong Wang bis & K.D. Hyde sp. nov.

Materials

Type status: Holotype. Occurrence: catalogNumber: MFLU 20-0261 ; recordedBy: Sun Ya-Ru; Taxon: scientificName: Distoseptisporabambusae; class: Sordariomycetes; order: Distoseptisporales; family: Distoseptisporaceae; Location: country: China; stateProvince: Guizhou; locality: Guiyang Medicinal Plants Garden ; verbatimElevation: 1100 m; Identification: identifiedBy: Yaru Sun; dateIdentified: 2019 Type status: Paratype. Occurrence: catalogNumber: MFLU 17-1653 ; recordedBy: Thambugala Kasun M.; Taxon: scientificName: Distoseptisporabambusae; class: Sordariomycetes; order: Distoseptisporales; family: Distoseptisporaceae; Location: country: Thailand; stateProvince: Chiangrai; locality: Doi Mae Salong ; verbatimElevation: 390 m; Identification: identifiedBy: Yaru Sun; dateIdentified: 2019

Description

Saprobic on culms of bamboo. Sexual morph: Undetermined. Asexual morph: Hyphomycetous (Figs 1 , 2 ). Colonies effuse, brown to dark-brown, hairy. Mycelium mostly immersed, composed of pale to dark brown, septate, branched, smooth, hyaline to subhyaline hyphae. Conidiophores macronematous, mononematous, septate, single or in groups of 2 or 3, erect, cylindrical, straight or slightly flexuous, olivaceous or brown, robust at the base 40-96 × 4-5.5 μm (x̅ = 69 × 5 μm, n = 10). Conidiogenous cells blastic, integrated, terminal, cylindrical, olivaceous or brown 9-19 × 4-5 μm (x̅ = 15 × 4.5 μm, n = 15). Conidia acrogenous, solitary, straight, obclavate, septate, thick-walled, rounded at the apex, truncate at the base, tapering towards apex, olivaceous or brown, 45-74 μm long (x̅ = 60.5 μm, n = 20), 5.5-9.5 μm at the widest (x̅ = 7.5 μm, n = 20).

Culture characteristics: Conidia germinated on PDA within 12 hours and germ tubes were produced from both ends. Colony reached 30 mm in 4 weeks at 26℃ on PDA media, circular, flat, surface rough, grey from above, brown from below, edge entire.

Notes

The morphological characteristics of Distoseptispora bambusae match well with the generic concept of Distoseptispora ( Su et al. 2016). Multi-gene analyses showed that D. bambusae is a phylogenetically-distinct species, most closely related to D. suoluoensis , a species isolated from submerged wood in a freshwater habitat ( Yang et al. 2018). Distoseptispora bambusae has shorter conidiophores (40-96 vs. 80-250 μm) and shorter conidia (45-74 vs. (65-) 80-125(-145) μm) than those of D. suoluoensis ( Yang et al. 2018). Our two specimens of D. bambusae were similar in morphology, but polyblastic conidiogenous cells were observed from the Chinese specimen, while the Thai specimen has only monoblastic conidiogenous cells. These may be due to geographical differences and the different observation period. Although the two strains clustered together with short branches in the phylogenetic tree, comparisons of ITS sequences showed that there are 3 bp (base pair) differences without gaps between two strains and we identified them as the same species following the guidelines for species delineation proposed by Jeewon and Hyde (2016).

Etymology

Bambusae, referring to the host.

Figures

Figure 1. Distoseptispora bambusae (MFLU 20 - 0261, holotype, collected from China) a, b. Colonies on natural substrate; c, d. Conidiophore with Conidia; e. Conidiophore; f. Conidiogenous cell; g - j. Conidia; k. Germinating conidium; l, m. Colony on PDA. Scale bars: c-e, k = 20 μm, f-j = 10 μm.

Figure 2. Distoseptispora bambusae (MFLU 17 - 1653, paratype, collected from Thailand). a, b. Colonies on natural substrate; c - g. Conidia attached to conidiophores; h - k. Conidia; l. Germinating conidium. Scale Bars: c = 50 μm, d-l = 25 μm.