Cytospora lumnitzericola Norphanphoun, T.C. Wen & K.D. Hyde

Norphanphoun, Chada, Raspe, Olivier, Jeewon, Rajesh, Wen, Ting-Chi & Hyde, Kevin D., 2018, Morphological and phylogenetic characterisation of novel Cytospora species associated with mangroves, MycoKeys 38, pp. 93-120 : 93

Cytospora lumnitzericola Norphanphoun, T.C. Wen & K.D. Hyde sp. nov. Figure 3

Etymology.

refers to the host where the fungus was isolated.

Holotype.

MFLU 18-1227

Isolated from leaf spot of Lumnitzera racemosa . Culture characteristic: Colonies on MEA reaching 5-6 cm diameter after 2 days at room temperature, colonies circular to irregular, medium dense, flat or effuse, slightly raised, with edge fimbriate, fluffy to fairly fluffy, white to grey from above, light yellow to green from below; not producing pigments in agar. Asexual morph: Conidiogenous cells (8 –)8.5– 14 × 0.6 –1.4(– 1.6) μm (x‒ = 8.4 × 1.4, n = 15), blastic, enteroblastic, flask-shaped, phialidic, hyaline and smooth-walled. Conidia (3.7 –)4– 4.5 × 1 –1.3(– 1.5) µm (x‒ = 4 × 1.2 µm, n = 30), unicellular, subcylindrical, hyaline, smooth-walled.

Material examined.

THAILAND, Phetchaburi Province, the Sirindhorn International Environmental Park, on leaf spot of Lumnitzera racemosa , 30 November 2016, Norphanphoun Chada NNS23-2a (MFLU 18-1227 dried culture, holotype; PDD, isotype); ex-type-living culture, MFLUCC 17-0508, ICMP.

Notes.

Based on the multigene phylogeny, Cytospora lumnitzericola is closely related to Cytospora thailandica (Fig. 1). Although conidial sizes of both species are similar, they have significant differences in nucleotides: ITS (26 nt), ACT (22 nt), and RPB2 (53 nt) (Table 5). The phylogeny derived from the ITS regions depicts C. lumnitzericola as an independent lineage close to C. brevispora CBS 116829 and C. eucalyptina CMW5882 (Fig. 2). In future, more collections are needed to confirm whether C. lumnitzericola can exist as a saprobe or endophyte as well as performing tests to confirm its pathogenicity.