Paracreptotrema rosenthali, Bautista-Hernandez, Christian E., Monks, Scott, Pulido-Flores, Griselda & Miranda, Rafael, 2015

Bautista-Hernandez, Christian E., Monks, Scott, Pulido-Flores, Griselda & Miranda, Rafael, 2015, A new species of Paracreptotrema (Digenea, Plagiorchiformes, Allocreadiidae) infecting two species of poeciliids in Rio Malila of the Rio Panuco basin, Hidalgo, Mexico, with a key to the species of the genus, ZooKeys 482, pp. 55-66 : 56-58

Taxon classification Animalia Plagiorchiida Allocreadiidae

Paracreptotrema rosenthali sp. n. Figure 1

Type material.

Holotype (CNHE 9263), 3 paratypes (CNHE 9264 to 9266), and 3 paratypes (HWML 75051 to 75054).

Other material examined.

Paracreptotrema blancoi Choudhury, Pérez-Ponce de León, Brooks & Daverdin, 2006 ( CNHE– 5315, Costa Rica; CNHE– 7682, México); Paracreptotrema heterandriae Salgado-Maldonado, Caspeta-Mandujano & Martínez-Ramírez, 2012 ( CNHE– 8242); Paracreptotrema mendezi (Sogandares-Bernal, 1955) Choudhury, Pérez-Ponce de León, Brooks & Daverdin, 2006 ( HWML– 22193, 22194); and Paracreptotrema profundulusi Salgado-Maldonado, Caspeta-Mandujano & Martínez-Ramírez, 2011 ( CNHE– 7684).

Type host.

Xiphophorus malinche Rauchenberger, Kallman & Morizot ( Poeciliidae ). Vouchers deposited in MZNA fish collection, University of Navarra, Spain ( Galicia et al. 2014).

Type locality.

Río Malila, tributary of Río Conzintla, northeastern Hidalgo, México (20°44'N; 98°43'W).

Site in host.

Intestine.

Other host.

Pseudoxiphophorus jonesii (Günther, 1874) (sensu Agorreta et al. 2013; = Heterandria jonesii ). Vouchers deposited in MZNA fish collection, University of Navarra, Spain ( Galicia et al. 2014).

Prevalence.

In Xiphophorus malinche , 7 of 88 infected (7.9%). In Pseudoxiphophorus jonesii , 1 of 36 infected (2.77%).

Etymology.

The species is named in honor of Gil G. Rosenthal, Department of Biology, Texas A&M University, College Station, Texas, and co-founder of the CICHAZ field station, for his friendship, contributions to the knowledge of species of Xiphophorus , and in recognition of his efforts to promote science in the Huasteca region of México.

Description.

[Based on 8 specimens] Body 720-940 (830 ± 83 n = 7) long, robust, aspinose. Anterior end rounded; body 350-550 (417 ± 66 n = 7), widening gradually, reaching maximum width at level of middle to posterior margin of acetabulum, terminating in narrower posterior end. Few, small, pigment spots in forebody. Oral sucker wider than long, subterminal, 105-160 (125 ± 20 n = 7) long, 130-175 (140 ± 16 n = 7) wide, with 2 papillae on posterior margin and several papillae along outer edge; opening subterminal, antero-ventrally directed. Average length of acetabulum, 170-230 (203 ± 18 n = 8) slightly greater than width, 180-225 (205 ± 15 n = 7), strongly muscular, sunken, visible externally by its rounded opening; tegument of acetabulum with fine striations radiating outward from acetabular opening. Ratio of length of oral sucker to length of acetabulum 1:1.4-1:2.0 (1:1.6) and width of oral sucker to width of acetabulum 1:1.3-1:1.7 (1:1.5). Prepharynx absent. Pharynx muscular, well developed, 45-55 (52 ± 4, n = 6) long, 38-70 (56 ± 11 n = 6) wide. Esophagus short, winding, 45-75 (57 ± 16, n = 3) long, 5-10 (n = 2) wide. Cecal bifurcation short distance anterior to acetabular margin. Ceca, mostly obscured by vitelline follicles, extend posterior to acetabulum, 725 (right side) to 800 (left side) long (n = 1), following curve of body, just surpassing posterior margin of ovary but not reaching testes (Fig. 1). Ovary 63-135 (104 ± 25 n = 8) long, 98-145 (111 ± 16 n = 7) wide, entire, dextral (4 of 8) or sinistral (4 of 8), 350-600 (435 ± 85 n = 8), 48%-64% (54% ± 6% n = 7) from anterior end of body, overlapping posterior margin of acetabulum in some specimens. Mehlis’ gland comprised of loose aggregate of gland cells, 25-63 (43 ± 12 n = 7) long, 25-68 (48 ± 15 n = 7) wide, partially obscured by vitelline follicles but more visible from dorsal perspective. Seminal receptacle postovarian, muscular. Laurer’s canal not observed. Uterus with loop extending to posterior margin of testes. Vitellarium follicular, in 2 lateral fields, each consisting of a roughly-formed double row of follicles, 1 row more lateral and 1 partially overlapping acetabulum dorsally. Vitelline follicles extend from level anterior to pharynx to middle of testes, to posterior margin of testes in some specimens. Lateral fields of vitellarium loosely confluent antero-dorsal to acetabulum, dorsally overlapping ceca, lateral margins of acetabulum, ovary, and testes. Follicles consist of granular vitelline cells; vitelline ducts connect to large median vitelline reservoir filled with vitellocalcyl cells. Eggs number about 20 to 40, well developed, ovoid, operculate, 40-70 (52 ± 7 n = 40) long, 25-45 (32 ± 4 n = 40) wide. Testes 2, irregular in form but not lobed, longer than wide, post-equatorial, posterior to and separated from ovary. Right testis 110-188 (137 ± 30 n = 8) long, 50-155 (88 ± 37 n = 7) wide, anterior margin 460-700 (571 ± 83 n = 8), 63%-78% (71% ± 5% n = 7) from anterior end of body. Left testis 105-188 (136 ± 32 n = 8) long, 68-168 (96 ± 38 n = 7) wide, anterior margin 490-760 (591 ± 88 n = 8), 69%-84% (73% ± 6% n = 7) from anterior end of body. Cirrus sac elongate, median, dorsal, 75-110 (91 ± 18 n = 3) long, 68-103 (80 ± 20 n = 3) wide, containing coiled tubular seminal vesicle that occupies most of space in cirrus sac (Fig. 1b). Genital pore median, between cecal bifurcation and anterior margin of acetabulum. Excretory vesicle I-shaped, tubular, narrow, reaching anteriorly to or slightly beyond level of mid-testes. Excretory pore terminal.