Cytospora ailanthicola X.L. Fan & C.M. Tian, Persoonia 45: 13 (2020)

Jia, Aoli, Chen, Baoyue, Lu, Hongyan, Xing, Yu, Li, Bin & Fan, Xinlei, 2024, Multigene phylogeny and morphology reveal three new species of Cytospora isolated from diseased plant branches in Fengtai District, Beijing, China, MycoKeys 101, pp. 163-189 : 163

Cytospora ailanthicola X.L. Fan & C.M. Tian, Persoonia 45: 13 (2020)

Fig. 3

Description.

Sexual morph: not observed. Asexual morph: Conidiomata pycnidial, immersed in the bark, scattered, producing black area on bark, circular to ovoid, with multiple locules, occasionally slightly erumpent through the surface. Conceptacle absent. Ectostromatic disc inconspicuous, grey to black, circular to ovoid, producing one ostiole per disc when mature. Ostiole in the centre of the disc, black, 50-110 µm in diam. Locules numerous, subdivided frequently by invaginations with common walls, circular to ovoid, 300-500 µm in diam. Conidiophores hyaline, unbranched, approximately cylindrical, 6.5-9 × 1-1.5 (av. = 8 ± 1.5 × 1.3 ± 0.2, n = 50) µm. Conidiogenous cells enteroblastic, phialidic. Conidia hyaline, elongate-allantoid, smooth, aseptate, 2.8-3 × 0.8-1.2 (av. = 3 ± 0.3 × 1 ± 0.2, n = 50) µm.

Culture characteristics.

Cultures on PDA are initially white, growing fast up to 5 cm after 3 d and entirely covering the 6 cm Petri dish after 7 d, with fluffy and whitish aerial mycelium, producing black pycnidia with cream to yellowish conidial drops exuding from the ostioles after 30 d. Pycnidia aggregated on surface.

Materials examined.

China, Beijing City, Fengtai Distinct, Qianling Mountain scenic area, 39°51'12.28"N, 116°5'17.74"E, from branches of Salix matsudana , 12 Apr 2023, A.L. Jia & X.L. Fan (BJFC CF20230400, living culture CFCC 59446) .

Notes.

Cytospora ailanthicola was first observed on branches of Ailanthus altissima in China by Fan et al. (2020). Lin et al. (2022) confirmed this species was a pathogen with strong virulence caused by poplar canker disease. In this study, CFCC 59446 was isolated from symptomatic branches of Salix matsudana in Beijing, which clustered in a well-supported clade with C. ailanthicola ex-holotype CFCC 89970 (ML/BI = 100/1). Therefore, CFCC 59446 is identified as C. ailanthicola .

Figures

Figure 3. Cytospora ailanthicola from Salix matsudana (BJFC CF 20230400) A, B habit of conidiomata on branch C transverse section through conidiomata D longitudinal section through conidiomata E, F conidiophores and conidiogenous cells G conidia. Scale bars: 1 mm (A); 200 µm (B); 100 µm (C, D); 10 µm (E-G)