Bezzia chilensis Spinelli & Ronderos, 2001

Anjos-Santos, Danielle, Diaz, Florentina, Spinelli, Gustavo Ricardo & nderos, Maria Marcela R, 2018, The immatures of Bezziachilensis Spinelli & Ronderos, 2001 (Diptera, Ceratopogonidae), ZooKeys 803, pp. 141-154 : 142-145

Bezzia chilensis Spinelli & Ronderos, 2001 Figs 1 a–f, 2 a–d, 3 a–g, 4 a–f

Bezzia chilensis : Spinelli and Ronderos 2001: 752 (male, female; Chile); Spinelli and Cazorla 2003: 47 (Argentina records); Borkent and Spinelli 2007: 93 (in Neotropical catalogue); Spinelli and Marino 2009: 205 (species list from Patagonia); Borkent 2016: 160 (in online world catalog).

Description of fourth instar larva

(Figs 1 a–f, 2 a–d). Head capsule (Figs 1 a–c, 2a) pale brown, about 2 times longer than wide, apex slightly bent ventrally, HL 0.30-0.32 (0.31, n = 7) mm; HW 0.12-0.19 (0.16, n = 7) mm, HR 1.70-2.41 (2.00, n = 6); SGW 0.083-0.116 (0.098, n = 6) mm; SGR 1.29-1.82 (1.66, n = 6). Setae simple, thin, medium to long sized, chaetotaxy as in Figure 1 a–c. Antenna bottom-shaped, small, length 0.01 (n = 3) mm. Labrum (Fig. 1c) longer than wide, not extending beyond hypostoma, with three pairs of anterolateral sensilla styloconica; palatum (Fig. 1d) with two pairs of closely spaced sensilla, one trichoidea, one campaniform sensillum; messors (Figs 1d, 2a) small, gently sclerotized, curved structures, situated away from mandibles, without scopae; palatal bar present (Fig. 1d), triangular, situated immediately posterior to messors. Mandible (Figs 1 c–f, 2a, d) hooked, curved, strongly sclerotized, apical tooth long, deep fossa mandibularis on ectal surface; MDL 0.045-0.073 (0.052, n = 6) mm, MDW 0.02 (n = 6) mm. Maxilla (Fig. 1 c–e) with pyriform sensillum, galeolacinia with lacinial sclerite 1 (Fig. 1e) and lacinial sclerite 2 (Fig. 1 c–f) with 2 setae, one medium-sized stout, other short; maxillary palpus (Fig. 1 c–f) cylindrical, with 4 subapical papillae, three medium-sized, one elongate. Hypostoma (Fig. 1 c–f) finely toothed, with 6 or 7 stout lateral teeth. Epipharynx (Fig. 2a, c) less massive, with 2 combs: ventral comb with 5 stout, short teeth, dorsal comb with 7 or 8 long, pointed teeth on posterior edge; lateral arms elongate; LAW 0.048-0.050 (0.049, n = 4) mm, DCW 0.025-0.038 (0.028, n = 4) mm. Hypopharynx (Fig. 2a) elongate, thin, gently sclerotized, arms slender, without fringe. Thoracic pigmentation uniformly pale yellowish. Caudal segment (Fig. 2b) about 2.7 times as long as wide, with one pair of long, stout setae “o”, one medium-sized, thin setae “i”, one pair of short, thin setae “l1”. CSL 0.51-0.57 (0.55, n = 5) mm, CSW 0.21-0.28 (0.25, n = 5) mm, CSR 1.88-2.71 (2.7, n = 5), OL 0.20-0.30 (0.27, n = 6) mm, OD 0.020-0.072 (0.049, n = 5) mm.

Description of pupa.

Female (Figs 3 e–g, 4 a–f). Habitus as male pupa (Fig. 3a). Exuviae brownish. Total length 3.14-3.91 (3.48, n = 13) mm. Head: Dorsal apotome (Fig. 3f) with disc surface bearing rounded small tubercle mesally, anterior margin slightly rounded, covered with stout, rounded spinules; posterior margin slightly concave, mesal portion with pair of raised areas; antenna extending posteriorly to midleg; mouthparts (Fig. 4a) with mandible well developed; palpus extending to posterolateral margin of labium; labium separated medially by labrum; apex of labrum slightly rounded; sensilla: dorsal apotomals (Fig. 3f): DA-1-H elongate, stout seta, located on rounded small tubercle, DA-2-H campaniform sensillum; DAL 0.08-0.10 (0.09, n = 10) mm; DAW 0.20-0.23 (0.22, n = 10) mm; DAW/DAL 2.24-2.64 (2.46, n = 10); two dorsolateral cephalic sclerites (Fig. 4b): DL-1-H short, stout seta, DL-2-H campaniform sensillum; clypeal/labrals (Fig. 4a): CL-1-H medium-sized thin seta CL-2-H long, thin seta; oculars (Fig. 4a): O-1-H short, stout seta, O-2-H campaniform sensillum, O-3-H long, stout seta. Cephalothorax rectangular, surface predominantly smooth with small spinules on mesonotum, between bases of respiratory organs. Length of cephalothorax 1.12-1.35 (1.22, n=13) mm, width 0.81-1.00 (0.92, n = 13) mm. Thorax: Respiratory organ (Fig. 3d, e) smooth, medium-sized, pale brown except distal 1/3 darker, about 3.05-5.70 (4.38, n = 13) times longer than broad, almost straight with rounded apex, with convoluted row of 30-35 (33, n = 13) pores closely abutting at apex and apicolateral 1/4 of respiratory organ; pedicel slender, P 0.024-0.040 (0.034, n = 13) mm; RO length 0.20-0.24 (0.22, n = 13) mm, RO width 0.04-0.08 (0.05, n = 13) mm; P/RO 0.10-0.18 (0.15, n=13); sensilla: three anteromedials (Fig. 4b): AM-1-T medium-sized, stout seta, AM-2-T long, thin seta, AM-3-T campaniform sensillum; one anterolateral (Fig. 4b): AL-1-T medium-sized, stout seta; dorsals (Fig. 4c): D-1-T, D-2-T, D-4-T, long, thin setae, D-3-T campaniform sensillum, D-5-T medium-sized, thin seta, all on small rounded tubercle; supraalar (SA-2-T) campaniform sensillum; metathoracic (Fig. 4d): M-3-T campaniform sensillum, near anterior margin of metathorax. Abdomen: Abdominal segments with dark spots, with simple setae, covered with very small spicules, segment 9 (Fig. 3g) approximately twice as long as wide, length 0.24-0.29 (0.27, n = 13) mm, width 0.14-0.20 (0.17, n = 13) mm; dorsal surface covered with pointed spicules; ventral surface smooth; terminal process moderately short, nearly straight, base wide, smooth, extreme tips darker, length 0.07-0.10 (0.09, n = 13) mm, width 0.03-0.04 (0.04, n = 13) mm; sensilla: tergite 1 (Fig. 4e) with two anteromesals: D-2-I medium-sized, thin seta, D-3-I long, thin seta; 5 posterior sensilla: D-4-I, D-7-I campaniform sensilla, D-5-I minute seta, D-8-I medium-sized, thin seta, D-9-I long, thin seta; 3 lateral sensilla: L-1-I long, thin seta, L-2-I, L-3-I medium-sized, thin setae; segment 4 (Fig. 4f): D-2-IV medium-sized, thin seta, D-3-IV long, thin seta, D-4-IV, D-7-IV campaniform sensilla, D-5-IV short, stout seta, D-8-IV medium-sized, stout seta, D-9-IV long, thin seta; L-1-IV short, stout seta, L-2-IV long, thin seta, L-3-IV, L-4-IV medium-sized, stout setae, all on bifid tubercles with wide base and pointed apex; V-5-IV, V-6-IV, V-7-IV medium-sized, stout setae, all on elongate tubercles; segment 9 (Fig. 3g) with D-5-IX campaniform sensillum.

Male (Fig. 3 a–d). Similar to female with usual sexual differences: Total length 2.68-3.77 (n = 15) mm. Dorsal apotome (Fig. 3b) darker with anterior margin slightly triangular, DAL 0.07-0.10 (0.08, n = 12) mm; DAW 0.18-0.22 (0.19, n = 12) mm, DAW/DAL 2.0-3.0 (2.4, n = 12). Cephalothorax: length 0.97-1.17 (1.09, n = 15) mm, width 0.70-0.85 (0.77, n = 13) mm. Respiratory organ (Fig. 3d), about 3.36-4.90 (4.01, n = 15) times longer than broad, P 0.020-0.036 (0.031, n = 15) mm; RO length 0.168-0.244 (0.205, n = 15) mm, RO width 0.040-0.060 (0.051, n = 15) mm; P/RO 0.106-0.196 (0.156, n = 15). Segment 9 (Figs 3a, c) darker, ventral surface covered anteriorly with pointed spicules, length 0.124-0.288 (0.241, n = 15) mm, width 0.120-0.248 (0.153, n = 15) mm; terminal process length 0.07-1.00 (0.08, n = 15) mm, width 0.032-0.044 (0.037, n = 15) mm; genital lobe short, each slightly longer than wide and apex anterior to base of terminal process.

Material examined.

Argentina, Neuquén Province, Parque Nacional Nahuel Huapi, río Cuyín Manzano, 40°44'13"S, 71°09'17"W, alt. 760 m, 06-II-2009, A. Siri, 2 females and 1 male (with pupal exuviae). Argentina, Chubut Province, Ruta Nacional 40, arroyo La Cancha, 42°45'35.9"S, 71°06'28.4"W, alt. 860 m, 13-II-2015, adults emerged in laboratory 14-II-2015, D. Anjos-Santos and P. Pessacq, 1 female, 3 males (with pupal exuviae); same data except adult emerged 15-II-2015, 1 male (with pupal exuviae); same data except adults emerged 17-II-2015, 2 females, 1 male (with pupal exuviae); same data except adults emerged 18-II-2015, 2 females, 1 male (with pupal exuviae); same data except adults emerged 17-II-2015, 1 female, 1 male (with pupal exuviae); same data except pupa emerged in laboratory 26-II-2015, adult emerged 28-II-2015, 1 female (with larval and pupal exuviae); same data except adult emerged 03-III-2015, 1 female (with pupal exuviae); Argentina, Chubut prov., Ruta Nacional 40, arroyo Madera, 42°39'57.59"S, 71°04'19.72"W, alt 930 m, 18-II-2015, pupa emerged in laboratory 23-II-2015, adult emerged 28-II-2015, D. Anjos-Santos and P. Pessacq, 1 male (with larval and pupal exuviae); same data except pupae emerged 23-II-2015, adults emerged 02-III-2015, 2 females (with larval and pupal exuviae); same data except pupa emerged 24-II-2015, 1 male (with pupal exuviae); same data except pupa emerged 26-II-2015, 1 male (with pupal exuviae); Argentina, Chubut Province, arroyo Montoso, 42°42'01.26"S; 70°48'12.36"W, alt. 630 m, 14-I-2016, pupa emerged in laboratory 16-I-2016, D. Anjos-Santos and P. Pessacq, 1 male (with pupal exuviae); same data except pupa emerged 17-I-2016, adult emerged 21-I-2016, 1 female (with larval and pupal exuviae); same data except pupa emerged 17-I-2016, adult emerged 22-I-2016, 1 male (with larval and pupal exuviae); same data except pupa emerged 20-I-2016, adult emerged 25-I-2016, 1 male (with larval and pupal exuviae).

Material examined by SEM.

Argentina, Chubut Province, Ruta Nacional 40, arroyo Madera, 42°39'57.59"S, 71°04'19.72"W, alt. 930 m, 18-II-2015, D. Anjos-Santos and P. Pessacq, 3 larvae, 1 male pupa.

Distribution.

Argentina (Salta, Neuquén, Río Negro and Chubut provinces); Chile (Valparaiso Province).