Lagenophrys turneri Kane, 1969

Clamp, John C. & Kane, John R., 2003, Redescription of Four Species of Lagenophryid Peritrichs (Ciliophora) from Australia and New Guinea, With Descriptions of Two New Species, Records of the Australian Museum 55 (2), pp. 153-168 : 165-166

Lagenophrys turneri Kane, 1969

Figs. 17–22 ; Tables 9, 10

Lagenophrys turneri Kane, 1969: 369 .– Clamp, 1991: 358. Circolagenophrys turneri .– Jankowski, 1986: 87.

Redescription. Lorica hemispheroidal, suboval to subcircular; if suboval, slightly to moderately wider than long. Rim of lorica very broad. Inner part of lorica rim slightly to moderately thickened; rim diminishing in thickness progressively to extremely thin edge. Lips of lorica aperture short, moderately arched; anterior lip slightly wider than posterior lip. Edges of both lips heavily thickened, smooth, without projections or indentations. Edge of anterior lip usually slightly to moderately thicker than edge of posterior lip. Anterior lip without crochets.

Trochal band of kinetosomes broken on right side in trophont; ends of break separated by wide gap. Infraciliature of infundibular polykinetids as follows: kinetosome rows of infundibular polykinetid 1 (P1) approximately equal in length, ending at cytostome; kinetosome rows of P2 equal in length, ending at abstomal curvature of P1. P2 not separated from P1 by wide gap; distance between row 3 of P1 and row 1 of P2 approximately equal to distances between rows within both polykinetids. P3 consisting of two kinetosome rows of approximately equal length. Rows of P3 slightly divergent at abstomal ends, closely parallel for remainder of length. P3 extending abstomally to point slightly beyond adstomal end of P2, ending adstomally at point slightly short of adstomal end of P1 and cytostome.

Macronucleus elongate, cylindroid, slightly curved, located along right edge of body, conforming to curve of edge of body. Micronucleus ovoid, located more often near centre of macronucleus than near either end of macronucleus.

Etymology. The species is named in honour of Mr J. Turner, who provided specimens of the host from the type locality.

Type material. LECTOTYPE, AUSTRALIA, Northern Territory, Katherine, Katherine R; 13 Sep 1963, J. Turner; on Macrobrachium rosenbergi (de Man) , gill lamellae . Lectotype slide with lectotype organism marked by inscribed circle (Erlich’s hematoxylin), AM P62821 . PARALECTOTYPE slide (Erlich’s hematoxylin), IPTC USNM 1004293 .

Other material examined. PAPUA NEW GUINEA, Gulf District, near Malalaua, Lake Kamu R.; May 1972, L.B. Holthuis; on M. rosenbergi , gill lamellae ( NMNH-CC USNM 141316 ). Voucher slides, (Heidenhain’s hematoxylin, protargol) AM P62822, P62886 and (Heidenhain’s hematoxylin) IPTC USNM 1004294 . Additional slides of this material remain in the senior author’s personal collection .

Remarks. The infundibular infraciliature of L. turneri ( Figs. 19 , 22 ) is essentially identical to that of L. eupagurus Kellicott, 1893 and L. callinectes Couch, 1967 ( Couch, 1973; Clamp, 1989). All three species also share the unusual characteristic of having the trochal band of kinetosomes broken on the right side in the trophont, leaving a wide gap ( Couch, 1967, 1973; Clamp, 1989). The trochal band is a continuous ring of kinetosomes in trophonts of other species of Lagenophrys that have been stained with protargol ( Clamp, 1987, 1988a,b, 1990a,b, 1992, 1994), as is typical in peritrichs. In addition, the shape and position of the macronucleus ( Figs. 17, 18 , 21 ) in L. turneri is the same as in L. eupagurus and L. callinectes .

from Papua New Guinea (n=25).

The edges of both lips of the lorica aperture are heavily thickened in L. turneri , L. eupagurus , and L. callinectes , but the latter two species have deep clefts in each lip that are lacking in L. turneri ( Figs. 17, 18 , 21 ; Couch, 1967; Clamp, 1989). The lips of the lorica aperture of L. turneri resemble those of L. callinectes more than those of L. eupagurus in having the anterior lip significantly thicker than the posterior lip ( Fig. 18 ; Tables 9, 10; Clamp, 1989). The differences in the morphology of the lorica aperture confirm the distinctness of L. turneri as a species while the several apparent synapomorphies that it shares with L. eupagurus and L. callinectes suggest that the three species may share a close phylogenetic relationship within the genus. Their restriction to decapod crustaceans as hosts reinforces this hypothesis.

Figures

Figs. 16–19.(16) Setonophrys nivalis, Erlich’s hematoxylin preparation. Dorsal view of lectotype individual from the type locality and host, AM P62819. (AL) Anterior lip of lorica aperture; (PL) posterior lip of lorica aperture; see Fig. 1 for explanation of other symbols. The micronucleus is the small, ovoid, darkly stained body located near the right end of the macronucleus. (17–19) Lagenophrys turneri. (17) Dorsal view of lectotype individual from the type locality and host; Erlich’s hematoxylin preparation, AM P62821. The lori ca aperture is partially open. (Cs) Boundary between infundibulum and cytostome (= ampulla); see Figs. 1, 16 for explanation of other symbols. The micronucleus is the small, ovoid, darkly stained body located near the posterior end of the macronucleus. (18) Dorsal view of individual from Papua New Guinea; Heidenhain’s hematoxylin preparation, AM P62822. The lorica aperture is almost closed. (19) Dorsal view of infundibular polykinetids of individual from Papua New Guinea; protargol preparation,AM P62886.The polykinetids are rows of kinetosomes and associated cilia (not shown) that are located on the wall of the infundibulum (Figs. 17, 21, 22). The rows of kinetosomes that constitute the polykinetidal infraciliature normally visible under the light microscope are shown as solid lines for the sake of convenience and clarity. (Ab) abstomal direction (= away from the cytostomal region); (Ad) adstomal direction (= toward the cytostomal region); (P1) polykinetid 1; (P2) polykinetid 2; (P3) polykinetid 3; (Pk) polykinety (the part of P1 that extends out onto the peristomial lip—only the part proximal to the infundibulum is shown); arrow with numerals shows the convention for numbering individual rows within each polykinetid; large arrow, adstomal curvature of P1.

Figs. 20–22. Lagenophrys turneri from Papua New Guinea. (20) Infested gill lamella of host at low magnification; Heidenhain’s hematoxylin preparation. (21) Dorsal view of single individual; Heidenhain’s hematoxylin preparation. See Figs. 1, 16 for explanation of symbols. (22) Dorsal view of single individual; protargol preparation. Large arrow, infundibular polykinetids; small arrow, haplokinety—the haplokinety is a band of dikinetids that extends into the infundibulum from the peristome, where it is the outer band of kinetosomes. It does not vary among sessiline peritrichs and is routinely omitted from views such as Fig. 19 for that reason. See Fig. 1 for explanation of symbols.