Cernosvitoviella atrata ( Bretscher, 1903 )

Dózsa-Farkas, Klára, Csitári, Bianka & Felföldi, Tamás, 2017, A new Cernosvitoviella species (Clitellata: Enchytraeidae) and its comparison with other Cernosvitoviella species from Sphagnum mires in Hungary, Zootaxa 4254 (3), pp. 322-338 : 329-330

Cernosvitoviella atrata ( Bretscher, 1903)

( Figures 1 C, 7–8)

Marionina atrata Bretscher, 1903: 5 , 17.

Enchytraeoides atratus (Bretscher) . Černosvitov 1928: 14 –16, text fig. 3, pl. I figs. 8, 9. Cernosvitoviella atrata (Bretscher) . Nielsen & Christensen 1959: 38 –39, figs 15–18. Kasprzak 1986: 109 –111, figs. 225, 252– 260. Chalupský 1992: 140 –141, fig. 7. Rota 1995: 193. Rota & Healy 1999: 31. Timm 2009: 148 –149. Schmelz & Collado 2010: 57, 62. Schmelz et al. 2015: 516 –517.

Material investigated: (in total 15 specimens), 1 specimen from site 1 (31.10. 2014), 7 specimens from site 2 (13.10.2014), 5 specimens from site 6 and 2 specimens from site 7 (31.03.2014).

Body 3–5 mm long, 150–200 µm wide at VIII and 170–250 µm at XII, segments 22–25. Chaetae ( Fig. 7 G) sigmoid with nodulus, up to 6–7 per bundle, 3 2–40 µm long. Clitellum absent dorsally and ventrally. In the prostomium about 8 large rounded papillae ( Fig. 7 B). Brain ( Fig. 7 A) incised posteriorly, about 10 µm long and 1.5 times longer than wide. Two pairs primary and two pairs secondary pharyngeal glands ( Fig. 8 A,B). Coelomocytes 14–20 µm long, variable, mostly oval, without ( Fig. 7 C) or with refractile granules, brown in transmittent light and often have long fine hyaline plasma threads ( Fig. 7 D). Dorsal vessel origin in XII–XIII. Blood light pink. Seminal vesicle absent. Sperm funnels funnel-shaped ( Fig. 7 E,H,I) 55–80 µm long, vas deferens 150–300 µm long and 15– 20 µm wide proximally and gradually tapering to 8–11 µm width distally. Male opening ( Fig. 7 E–F) surrounded with glands, diameter 35–55 µm. Spermatozoa 30–50 µm long, heads 15–25 µm. Spermathecae ( Figs. 1 C, 8A–D) confined to V, ampullae elongate, about as long or somewhat shorter than the length of the ectal ducts, sperm arranged lengthwise.

Figures

FIGURE 1. Spermathecae of Cernosvitoviella species. A. C. farkasi sp. n. B. C. aggtelekiensis. C. C. atrata. D. C. crassoductus. E. C. minor s. l. with small spermatheca. F. C. minor s. l. Scale bars 50 µm.

FIGURE 7. Micrographs of Cernosvitoviella atrata. A. Brain. B. Prostomial papillae (arrow). C. Coelomocytes with fine vesicles. D. Coelomocytes with long fine hyaline plasma threads (arrows), in cytoplasma dark, refractile granules. E. Funnelshaped sperm funnels (white arrows), sperm duct, male pores with glands surrounding (black arrows). F. Male pore (white arrow), surrounding large glands (black arrow) in 100 x magnification. G. Chaetae. H. Sperm funnels (white arrows) and sperm ducts (black arrow, m = male copulatory organ). I. Sperm funnel and fore part of the ductus (arrow) in 100 x magnification. All micrographs in vivo. Scale bars in A – C, E, H 50 µm, in D, F – G, I 20 µm.

FIGURE 8. Micrographs of Cernosvitoviella atrata. A – B. Spermathaecae (arrows, p = primary pharyngeal glands, sp = secondary pharyngeal glands). C – D. Spermathaecae (ampullae marked with white arrows, ectal ducts marked with black arrows) D. in 100 x magnification. All micrographs in vivo. Scale bars in A – C 50 µm, in D 20 µm.