Streptomyces krungchingensis, Sripreechasak & Phongsopitanun & Tamura & Tanasupawat, 2017

Sripreechasak, Paranee, Phongsopitanun, Wongsakorn, Tamura, Tomohiko & Tanasupawat, Somboon, 2017, Streptomyces krungchingensis sp. nov., isolated from soil, International Journal of Systematic and Evolutionary Microbiology 67 (1), pp. 50-54 : 53

DESCRIPTION OF STREPTOMYCES KRUNGCHINGENSIS SP. NOV.

Streptomyces krungchingensis (krung.ching.en′ sis. N.L. masc. adj. krungchingensis pertaining to Krung Ching Waterfall National Park in Nakhon Si Thammarat Province of Thailand, where the type strain was isolated).

Aerobic, mesophilic, filamentous, Gram-stain-positive actinomycete that produces extensively branched substrate and aerial mycelia. Flexuous spore chains are observed on aerial mycelia. The spore surface is smooth. White to light-greenish grey aerial mass is produced on ISP2, ISP3, ISP4 and nutrient agar but absent on ISP5, ISP6 and ISP7 media. The substrate mycelia appear greyish yellow on ISP2, ISP7 and nutrient agar, light olive brown on ISP3 and ISP4, brilliant yellow on ISP5 and greyish olive green on ISP6. Yellow to brown pigment is observed on most tested media except ISP5. Reduces nitrate to nitrite and hydrolyses starch but does not hydrolyse milk. Liquefaction of gelatin is weakly positive. Utilizes cellobiose, fructose, myo -inositol, D- mannitol, D- mannose, raffinose, ribose and sucrose as sole carbon sources, weakly utilizes salicin and D- xylose but does not utilize L- arabinose, cellulose, melezitose or D- sorbitol. Shows enzyme activities of acid phosphatase, alkaline phosphatase, esterase (C4), b-galactosidase, leucine arylamidase and N -acetyl-b- glucosaminidase, shows weak activities of esterase lipase (C8), b- glucosidase, naphthol-AS-BI-phosphohydrolase and valine arylamidase but does not show activities of a- chymotrypsin, cystine arylamidase, a- fucosidase, a- galactosidase, a- glucosidase, b- glucuronidase, lipase (C14), a- mannosidase or trypsin. Growth occurs at pH 5–9, at 15–37 Ǫ C and with 0–6 % (w/ v) NaCl. The cell-wall peptidoglycan contains LL- diaminopimelic acid. Ribose and glucose are observed as whole-cell sugars. The menaquinones are MK-9(H 8), MK-9(H 6) and MK-9 (H 4). The N -acyl type of muramic acid is acetyl. Phospholipids are diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, two unknown lipids and an unidentified aminolipid. The major cellular fatty acids are anteiso-C 15: 0, C 16: 0, iso-C 16: 0, iso-C 15: 0 and iso-C 14: 0.

The type strain, KC-035 T (= NBRC 110087 T = KCTC 29 503 T = TISTR 2402 T), was isolated from soil collected from Krung Ching Waterfall National Park , Nakhon Si Thammarat Province, Thailand. The DNA G+C content of the type strain is 72 mol% .