Chordodes colchis, Kintsurashvili, Nunu & Schmidt-Rhaesa, Andreas, 2017

Kintsurashvili, Nunu & Schmidt-Rhaesa, Andreas, 2017, Chordodescolchis (Nematomorpha, Gordiida), a new species from Georgia, Zoosystematics and Evolution 93 (2), pp. 325-331 : 325-329

Chordodes colchis sp. n.

Holotype.

Male from Imereti, deposited in the collection of Institute of Zoology, Ilia University, Tbilisi, Georgia under accession number 114.

Material investigated.

Holotype, nine female paratypes (accession numbers 100, 113, 115-117, 119-120, 126-127) and five male paratypes (accession numbers 95, 121, 123-125), all deposited in the collection of Institute of Zoology, Ilia University, Tbilisi, Georgia; 14 specimens (7 females and 7 males) deposited in the collection of the Zoological Museum, Hamburg, Germany under accession numbers ZMH V13418-V13431.

Type locality.

Imereti: village Kldeti, village Mirontsminda; Adjara: village Charnali; Tbilisi.

Etymology.

The name colchis is an association with ancient kingdom Colchis, which includes modern West Georgia, where most of the specimens of the species were found.

Description of the male.

The body length is 100-210 mm, the width is 1-1.9 mm (n=13). The body looks black with naked eye, however when observed under the microscope, it is very dark brown. The apical part of the body is white and gradually changes into darker color towards the end of the body. There are numerous dark structures on the body surface, these are the clusters of crowned areoles (Fig. 2A). The shape of the posterior end varies to some degree. In some specimens, when observed from the ventral side, the posterior end has a median longitudinal furrow and therefore both sides look partially divided (Fig. 2 B–D). In other specimens, the posterior end is less strongly divided (Fig. 2F). The cloaca is located 300 µm anterior from the apex (Fig. 2C, F). Short bristles, lying flat on the ventral surface, are present posterior of the cloacal opening while longer bristles are present lateral of the cloacal opening (Fig. 2C). The cloacal opening itself is surrounded by bristles which originate inside the opening, are attached to the lateral wall of the cloacal duct and then stand free around the opeing (Fig. 2E). Bristles are flat and wide at their base and then divide into 4, 5, 6 or more branches (Fig. 2E).

There are five types of areoles in the cuticle. Simple areoles are spread on entire surface of horsehair worm body. They are numerous. These are cylindrical struc tures, whose apical surface is spherical, oval or polygonal. Simple areoles measure 10-18 µm. Some simple areoles have small bristles on the apical surface (1-2,5 µm) (Fig. 3A, B, D). The cuticle between areoles is structured into cords (Fig. 3 B). Tubercle areoles are located between simple areoles (Fig. 3A, D). Tubercle areoles have finger-shaped apical projections (~10-20 µm). In some areoles these appendices are very small and difficult to measure. They look like big light-refracting dots. Thorn areoles are well distinguished from the rest by their large size and location (Fig. 3A, B, D). They are located on the ventral surface of the body. Areoles have two parts: a spherical basal portion, with the diameter of 20-25 µm at its widest point and 15 µm at its narrower part. Each thorn is raised from the basal structure which resembles an areole. The size of thorns varies from 12.5 to 20-25 µm. The size of the entire thorn areoles varies distinctly from 15 µm to 42.5 µm. The shapes of thorns are also different: some are straight, some are slightly bent, and some are curved (Fig. 3B). Often, thorns are detached from the basal portion, which can be observed under the light microscope. In this case, the basal part of the areoles is well distinguished from simple areoles by its large size, and spherical light refracting structure on the top. The location of thorn areoles is especially interesting. They are situated on the ventral surface on both sides of the midline of the body, almost opposite to each other (Fig. 3A).

Scattered over the entire surface of the body are clusters of areoles, which are easily distinguished from the rest of the areoles (Figs 2A, 3A, C, D). There are two (rarely, one, relatively large) crowned areoles in the center, ~ 7-10 µm apart. There is one very small light refracting thorn between them. The surface of the crowned areoles is spherical or oval. The largest diameter of each areole is ~ 20-25 µm, the smallest diameter is ~ 15-17 µm. Crowned areoles have a cylindrical shape, which is raised from the surface of the body. There is a crown of filaments on top of the surface. Filaments are numerous and vary in size. The longest filaments are about 15-17 µm. The rest of the filaments are smaller, twisted and look like a bush (Fig. 3C). Crowned areoles are surrounded by two rows of circumcluster areoles. There are about 6-7 areoles in the inner row immediately surrounding the crowned areoles. The outer row comprises 12-13 areoles. However, sometimes circumcluster areoles are arranged around crowned areoles in one row. In those cases the number of circumcluster areoles is 20-21. The size of circumcluster areoles is much smaller than the crowned areoles. Circumcluster areoles look like simple areoles, but they are more projected from the surface of the body and have a dark color. They are curved in the direction of crowned areoles. Circumcluster areoles have very thin, short bristles on the apical surface (Fig. 3C).

Description of the female: The body length is 140-340 mm, the width at the midpoint is 2-2.5 mm (n=16). The body is tapering towards the anterior end (Fig. 4A). The posterior end is spherical, with a cloaca in the center (Fig. 4B, E). The cuticle around the cloaca is smooth. Color of the body is light brown throughout the length of the body with the exception of the apical end, where ~1.5-2 mm of the body is white, gradually changing into brown. Also, the color of the posterior end is whitish.

There are six types of areoles in the cuticle. The cuticle consists of many small simple areoles. They are round or oval shaped. Simple areoles have short, fine bristles, similar to males. The cuticle between the areoles is structured into cords. There are relatively large areoles with small finger-shaped projections situated among simple areoles. These are tubercle areoles, which are also found in males. However, in contrast of male specimens, there are a number of thin tubercles that appear to originate between the areoles in the anterior end of most specimens (Fig. 4C, D). In some specimens they are clearly oriented towards the anterior with their tip, in others the orientation is more irregular. In addition, female specimens have some bristles behind the region with areoles in the posterior end (Fig. 4E, F).

There are clusters of crowned areoles with short filaments on the surface of entire body. In contrast of males, females have clusters of crowned areoles with very long and relatively stout apical filaments on the dorsal and ventral central line (Fig. 4G, H). The structure of circumcluster areoles is identical to those of males (Fig. 4G). Thorn areoles, as described in males, are also present in females. Their structure and location is similar in both sexes. However, in females, the number of thorn areoles is lower. In addition, they are difficult to observe due to their location under the long apical filaments of crowned areoles.