Talaromyces resedanus (McLennan and Ducker) A.J. Chen, Houbraken & Samson, 2020

Sun, Bing-Da, Chen, Amanda J., Houbraken, Jos, Frisvad, Jens C., Wu, Wen-Ping, Wei, Hai-Lei, Zhou, Yu-Guang, Jiang, Xian-Zhi & Samson, Robert A., 2020, New section and species in Talaromyces, MycoKeys 68, pp. 75-113 : 75

Talaromyces resedanus (McLennan and Ducker) A.J. Chen, Houbraken & Samson comb. nov. Fig. 10

Penicillium resedanum McLennan and Ducker, Aust. J. Bot. 2: 360. 1954. Basionym.

= Talaromyces omanensis Halo, Maharachch., Al-Yahyai and Al-Sadi, Phytotaxa 404: 192. 2019.

Typus.

Australia, Frankston in solo arenoso acido, Holotype IMI 062877, culture ex-holotype CBS 181.71 = DTO 376-A7 = ATCC 22356 = FRR 578 = IMI 062877 = NRRL 578.

Additional material examined.

Sweden, soil in greenhouse, 1989, isolated by O. Constantinescu, culture CBS 184.90 = DTO 376-A8 = UPSC 2879.

ITS barcode.

MN864280. Alternative identification markers: BenA = MN863349, CaM = MN863326, RPB2 = MN969214.

In.

Talaromyces section Subinflati

Colony diam, 7 d (mm).

CYA 19-21; CYA 30 °C 18-20; CYA 37 °C 8-11; MEA 23-25; MEA 30 °C 15-21; OA 26-28; YES 17-19; CREA No growth; CYAS 5-7; DG18 8-9.

Colony characters.

CYA 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium sulphur yellow (15) at center, white at edge; texture floccose; sporulation absent; soluble pigments absent; exudates clear droplets; reverse saffron (10). MEA 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white and sulphur yellow (15); texture floccose; sporulation sparse; conidia en masse white to greyish yellow-green (68); soluble pigments absent; exudates clear droplets; reverse saffron (10). YES 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white and buff (45); texture floccose; sporulation sparse; conidia en masse white to greyish yellow-green (68); soluble pigments absent; exudates clear droplets; reverse saffron (10). DG18 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white; texture floccose; sporulation absent; soluble pigments absent; exudates absent; reverse cream white. OA 25 °C, 7 d: Colonies moderately deep, plane; margins entire; mycelium white and sulphur yellow (15); texture floccose; sporulation absent; soluble pigments absent; exudates clear droplets; reverse white. CREA 25 °C, 7 d: No growth.

Micromorphology.

Conidiophores monoverticillate, stipes smooth, 50-150 × 3-4.5 μm, phialides 3-9, flask shaped, 9-12 × 3-3.5 μm; conidia finely rough, ellipsoidal, 2-3 × 1.5-2 μm. Ascomata not observed.

Notes.

Talaromyces resedanus grows restrictedly on DG18 and does not grow on CREA, two features shared with other taxa in section Subinflati . The monoverticillate conidiophores can differentiate T. resedanus from all reported section Subinflati species. Talaromyces aerugineus , T. flavus , T. intermedius , T. rotundus , T. tardifaciens also produce monoverticillate conidiophores, all of them except T. aerugineus can produce ascospores. Talaromyces aerugineus differs from T. resedanus by its shorter conidiophores (10-20 × 2.5-5 μm) and large, globose to ellipsoidal conidia (3-8.5 × 2.5-5 μm).

This species was introduced as Penicillium resedanum ( McLennan et al., 1954). Yilmaz et al. (2014) listed it as doubtful species because the ex-type culture CBS 181.71 was not viable at that time. We requested the lyophilized culture of CBS 181.71 and CBS 184.90 deposited in nitrogen, and successfully resurrected them. The concatenated alignment and the single gene phylogenies proved its assignment in section Subinflati . Talaromyces omanensis described by Halo et al. (2019) shares ITS, BenA and CaM (all 100% similarity) sequences with T. resedanus CBS 184.90, have 99.7% (576/578), 98.3% (357/363), 98.8% (487/493) similarity with T. resedanus CBS 181.71T. Its type culture SQUCC 13153 showed good sporulation on CYA and MEA and thus displayed green colony. The monoverticillate conidiophores, size and shape of stipes, phialides and conidia of T. omanensis resemble those of T. resedanus , except that conidiophores of T. omanensis are rough under scanning electron microscope (SEM) ( Halo et al. 2019). The photo plate of T. omanensis showed smooth conidiophores under microscope. Based on the molecular and morphological similarity, we considered T. omanensis a synonym of T. resedanus .

Figures

Figure 10. Talaromyces resedanus CBS 181.71 T a colonies from left to right (top row) CYA, MEA, YES and OA; (bottom row) CYA reverse, MEA reverse, DG 18 and CREA b-g conidiophores and conidia. Scale bars: 10 μm (b-g).