Cyanonectria bispora Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang, 2023

Zhang, Zhi-Yuan, Li, Xin, Chen, Wan-Hao, Liang, Jian-Dong & Han, Yan-Feng, 2023, Culturable fungi from urban soils in China II, with the description of 18 novel species in Ascomycota (Dothideomycetes, Eurotiomycetes, Leotiomycetes and Sordariomycetes), MycoKeys 98, pp. 167-220 : 167

Cyanonectria bispora Zhi.Y. Zhang, Y.F. Han & Z.Q. Liang sp. nov.

Fig. 22

Etymology.

In reference to its production of both macroconidia and microconidia.

Type.

China: Yunnan Province, Dali City, Dali Bai Autonomous Prefecture People's Hospital 25°57'89"N, 100°22'16"E, soil, 3 Sep 2019, Z.Y. Zhang (HMAS 351875 holotype designated here, ex-type living culture CGMCC 3.20774 = GZUIFR 21.908) .

Description.

Culture characteristics (14 d at 25 °C): Colony on PDA 42-46 mm diam., grey (29B1), flocculent, aerial mycelium sparse, substrate mycelium abundant, fimbriate; reverse grey (29C1). Colony on MEA 15-19 mm diam., grey (29B1) to white (29A1) from centre to margin, fluffy, margin dentate; reverse grey (29F1) to white (29A1) from centre to margin. Colony on OA 63 mm diam., yellowish-white (4A2), felty, rounded, margin regular; reverse pale yellow (4A3).

Hyphae branched, septate, hyaline, smooth, 1.0-5.0 μm diam. Conidiophores mononematous (aerial conidiophores) or grouped on sporodochia. Monophialides arising from aerial hyphae, hyaline, smooth-walled, solitary or connected by pronounced connectors, sometimes septate, cylindrical, sometimes curved irregularly, neck broadly tapering towards the apex, 5.5-39.0 μm long, 1.0-3.5 μm wide at the base, ca. 1.0-2.5 μm near the aperture. Sporodochia of branched conidiophores with solitary or whorls of 2-3 terminal monophialides. Phialides of sporodochia cylindrical or bottle-shaped, 13.5-29 μm long, 2.0-3.5 μm wide at the base, 2.5-4.0 μm in middle, 1.0-2.0 μm wide near the conidiogenous aperture. Microconidia hyaline, smooth, aseptate, cylindrical, fusiform, sometimes irregular, 5.0-14.5 × 2.5-6.0 μm (av. 10.4 × 4.6 μm, n = 50). Macroconidia hyaline, smooth, typically with the central and basal part nearly straight, rarely gently curved throughout, with a more or less pronounced pedicellate foot cell and an inequilateral fusoid or hooked apical cell, aseptate or 1-3(-4) septate, 23.5-40.5 × 3.0-5.5 μm (av. 38.6 × 4.8 μm, n = 50). Chlamydospores not observed. Sexual morph not observed.

Additional specimens examined.

China: Guangxi Zhuang Autonomous Region , Guilin City, Yucai Campus of Guangxi Normal University 25°26'64"N, 110°32'70"E, soil, 30 Aug 2019, Z.Y. Zhang, GZUIFR 21.909 .

Notes.

The species is described here, based on its morphology of the asexual morph. In the phylogenetic analysis (Fig. 21 ), Cyanonectria bispora is nested in the genus Cyanonectria and sister to C. cyanostoma and C. buxi . Morphologically, C. bispora can be distinguished from C. cyanostoma and C. buxi by the production of macroconidia and microconidia ( Samuels et al. 2009). In addition, C. cyanostoma and C. buxi are reported from Europe (Belgium, France, Germany and Slovenia) and associated with B. sempervirens , while C. bispora was isolated from soil in China.

Figures

Figure 21. Concatenated phylogeny of the ITS, LSU, RPB 2 and EF 1 A gene regions of species in Cyanonectria and its allied genera. Thirty-three strains are used. The tree is rooted in Ilyonectria destructans (CBS 264.65) and I. capensis (CBS 132815). The tree topology of the BI was similar to the ML analysis. Bayesian posterior probability (≥ 0.7) and ML bootstrap values (≥ 70 %) are indicated along branches (PP / ML). Novel species are in blue and bold font and " T " indicates type derived sequences.

Figure 22. Cyanonectria bispora (from ex-holotype CGMCC 3.20774) a-c upper and reverse views of cultures on PDA, MEA and OA 14 d after inoculation d-f monophialide and conidia g, h polyphialides with multiple conidiogenous loci i sporodochial conidiophore and conidiogenous cells j conidia. Scale bars: 10 µm (d-j).